Product Description
Skip the heat block and water bath. These Zymo Research premade JM109 competent cells transform by simply mixing DNA with cells and spreading onto a plate, with efficiencies above 10^8 transformants per µg pUC19. They are a convenient choice for routine plasmid work, from simple subcloning to building larger libraries.
This listing is for catalog number T3003: Mix & Go! Competent Cells - JM109, 10 x 100 µl. JM109 is a widely used cloning host, and these chemically competent cells are prepared by a method that removes the need for heat shock. They suit cloning, subcloning, PCR fragment cloning and library construction. Available as 10 x 100 µl tubes or a 96 x 50 µl format for higher throughput. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- No heat shock – mix DNA with cells and plate directly.
- High efficiency – greater than 10^8 transformants per µg pUC19.
- Premade and ready – chemically competent cells arrive ready to use.
- Versatile cloning host – suited to cloning, subcloning and library construction.
- Two formats – 10 x 100 µl tubes or 96 x 50 µl.
Specifications
| Catalog Number | T3003 |
| Brand | Zymo Research |
| Description | Mix & Go! Competent Cells - JM109, 10 x 100 µl |
| Strain | E. coli JM109 |
| Transformation efficiency | >10^8 transformants/µg pUC19 |
| Heat shock | Not required |
| Formats | 10 x 100 µl or 96 x 50 µl |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Available Sizes & Components
Applications
- Plasmid cloning
- PCR fragment cloning
- Subcloning
- Library construction
