Product Description
LIGHT LABS is proud to offer the Zymo Research OneStep™ PCR Inhibitor Removal Kit (Cat # D6031) — the fast, reliable solution for removing potent PCR inhibitors from DNA and RNA preparations in a single spin-column step. Engineered for researchers working with challenging environmental, soil, fecal, plant, and other complex sample types, this kit eliminates the inhibitory compounds that cause PCR failure, poor sequencing results, and unreliable downstream data.
The D6031 is the updated version of the widely cited D6030, featuring a simplified no-prep-buffer workflow and improved inhibitor removal efficiency. LIGHT LABS is your trusted source for Zymo Research products, offering fast shipping and expert support from Englewood, CO.
KEY FEATURES
- Removes the most common and potent PCR inhibitors including polyphenolic compounds, humic/fulvic acids, tannins, and melanin
- Fast, one-step spin-column protocol — cleanup completed in approximately 5 minutes
- No prep buffer required — simplified workflow compared to previous generation (D6030)
- High DNA and RNA recovery: 50–90%
- Compatible with downstream applications including PCR, qPCR, RT-PCR, sequencing, and NGS
- Processes 50–200 µl of sample per column; same column can be reused for the same sample
- Requires microcentrifuge at 3,500 x g
- Works with DNA and RNA preparations from any source
- Made in the USA by Zymo Research
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | D6031 |
| Brand | Zymo Research |
| Kit Name | OneStep™ PCR Inhibitor Removal Kit |
| Format | Spin Column (Zymo-Spin IV-IR HRC Filter) |
| Number of Preps | 50 |
| Elution Volume | 50–200 µl |
| DNA Recovery | 50–90% |
| RNA Recovery | 50–90% |
| Processing Time | ~5 minutes |
| Equipment Required | Microcentrifuge (3,500 x g) |
| Sample Types | DNA/RNA from environmental, soil, fecal, plant, and other inhibitor-rich samples |
| Inhibitors Removed | Polyphenolics, humic/fulvic acids, tannins, melanin, and other enzymatic inhibitors |
| Downstream Applications | PCR, qPCR, RT-PCR, sequencing, NGS, reverse transcription |
| Storage | Room temperature |
| Distributed By | LIGHT LABS — lightlabsusa.com |
APPLICATIONS
- Environmental DNA (eDNA) research
- Soil and sediment DNA/RNA cleanup
- Fecal and gut microbiome sample processing
- Plant and tissue DNA purification cleanup
- Food safety and agricultural testing
- Wastewater surveillance and water quality analysis
- Forensic DNA sample preparation
- Clinical and veterinary diagnostics sample prep
- Any workflow where PCR inhibition is a concern
ORDERING INFORMATION
| Cat # | Description | Size |
|---|---|---|
| D6031 | OneStep™ PCR Inhibitor Removal Kit | 50 Preps |
| D6030 | OneStep™ PCR Inhibitor Removal Kit (previous generation) | 50 Preps |
| D6035 | OneStep-96™ PCR Inhibitor Removal Kit | 2 x 96 Preps |
ABOUT LIGHT LABS
LIGHT LABS (lightlabsusa.com) is your trusted distributor of premium laboratory consumables and equipment, located in Englewood, CO 80111. Contact us at 1-800-286-3103. LIGHT LABS distributes leading brands including Zymo Research, MTC Bio, CELLTREAT, Benchmark Scientific, IKA, DWK Life Sciences (Kimble/Wheaton), Diversified Biotech, Accuris Instruments, and more.
