Product Description
A failed PCR from a difficult soil, plant or tissue extract often comes down to carryover contaminants rather than missing template. This Zymo Research clean-up kit rescues those samples by stripping out humic and fulvic acids, polyphenols, tannins and melanin from DNA or RNA preparations so amplification and reverse transcription can proceed.
This listing is for catalog number D6035: OneStep-96 PCR Inhibitor Removal Kit, 2 x 96 Preps. The kit uses specially designed column matrices that capture inhibitory compounds while the nucleic acid passes through. The workflow is simply apply, spin and collect, so it adds only minutes to an existing extraction. It is offered as a 50-prep column kit and as a 96-well plate version for high-throughput labs cleaning up large numbers of environmental or forensic extracts. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Targets common inhibitors – Removes polyphenolic compounds, humic and fulvic acids, tannins and melanin.
- Works on DNA and RNA – Cleans up both nucleic acid types ahead of PCR or RT reactions.
- One-spin workflow – Apply the sample, centrifuge and recover the cleaned eluate.
- Two formats – Available as individual columns or a 96-well plate for higher throughput.
- Complete kit – Supplies all components needed for inhibitor removal.
Specifications
| Catalog Number | D6035 |
| Brand | Zymo Research |
| Description | OneStep-96 PCR Inhibitor Removal Kit, 2 x 96 Preps |
| Formats | Spin column (50 preps); 96-well plate (2 x 96 preps) |
| Compatible samples | DNA and RNA preparations |
| Contaminants removed | Polyphenolic compounds, humic/fulvic acids, tannins, melanin |
| Downstream use | PCR, RT |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- Cleaning soil and environmental DNA extracts
- Rescuing inhibited PCR and RT-PCR reactions
- Plant and tissue extract clean-up
- Forensic sample preparation
- Pre-sequencing nucleic acid clean-up
