Product Description
Speed through media prep with a fast-flowing membrane built for cell culture. These sterile vacuum filter systems combine a filter funnel and receiving bottle, letting you sterilize or clarify media, buffers and supplements and store the filtrate without a transfer step. Five volumes from 50 mL to 1000 mL and three pore sizes fit both small batches and bulk preparation.
This listing is for NEST catalog number 343003: Vacuum Filter System with Receiving Bottle, 500 mL, 0.45 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1). The polyethersulfone (PES) membrane offers low protein binding with a high flow rate, and the cup is molded from virgin polystyrene with a 45 mm neck. PES is not for use with chloroform, esters, amides, strong acids or strong bases. Units are E-beam sterilized to SAL 10⁻⁶, individually bagged, made in a cGMP, ISO 13485 facility and lot tested free of pyrogens, cytotoxins, DNase, RNase and DNA. Order it from LIGHT LABS alongside the rest of your NEST plastics and consumables.
Key Features
- Fast Flow PES – high flow rates shorten filtration time for large media volumes
- Low Protein Binding – preserves growth factors and serum proteins in media
- Complete System – filter funnel and receiving bottle in one sterile unit
- Three Pore Sizes – 0.10 µm, 0.22 µm and 0.45 µm options
- Sterile and Tested – E-beam sterilized to SAL 10⁻⁶ and lot tested free of pyrogens and nucleases
Specifications
| Catalog Number | 343003 |
| Brand | NEST Scientific |
| Description | Vacuum Filter System with Receiving Bottle, 500 mL, 0.45 µm PES Membrane, Low Protein Binding, Sterile |
| Sold As | Case of 12 (12 x 1) |
| Volume | 500 mL |
| Pore Size | 0.45 µm |
| Sterile | Yes |
| Units per Pack | 1 |
| Units per Case | 12 |
| Membrane | PES (polyethersulfone) |
| Configuration | Filter funnel with receiving bottle |
| Housing | Virgin polystyrene cup with cap |
| Neck | 45 mm |
| Chemical Limits | Not for chloroform, esters, amides, strong acids or strong bases |
| Sterilization | E-beam, SAL 10⁻⁶ |
| Certified free of | Pyrogens (USP 85), cytotoxins (USP 87/88), DNase, RNase, DNA, BSE/TSE; animal-origin components |
Available Options
- 347002 – Vacuum Filter System with Receiving Bottle, 50 mL, 0.10 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 347001 – Vacuum Filter System with Receiving Bottle, 50 mL, 0.22 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 347003 – Vacuum Filter System with Receiving Bottle, 50 mL, 0.45 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 346002 – Vacuum Filter System with Receiving Bottle, 150 mL, 0.10 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 346001 – Vacuum Filter System with Receiving Bottle, 150 mL, 0.22 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 346003 – Vacuum Filter System with Receiving Bottle, 150 mL, 0.45 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 342002 – Vacuum Filter System with Receiving Bottle, 250 mL, 0.10 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 342001 – Vacuum Filter System with Receiving Bottle, 250 mL, 0.22 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 342003 – Vacuum Filter System with Receiving Bottle, 250 mL, 0.45 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 343002 – Vacuum Filter System with Receiving Bottle, 500 mL, 0.10 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 343001 – Vacuum Filter System with Receiving Bottle, 500 mL, 0.22 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 344002 – Vacuum Filter System with Receiving Bottle, 1000 mL, 0.10 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 344001 – Vacuum Filter System with Receiving Bottle, 1000 mL, 0.22 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 344003 – Vacuum Filter System with Receiving Bottle, 1000 mL, 0.45 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
Applications
- Cell culture media sterilization
- Media clarification
- Buffer and reagent filtration
- Serum and supplement filtration
- Tissue culture prep
- Mycoplasma reduction (0.10 µm)
