Product Description
Speed through media prep with a fast-flowing membrane built for cell culture. These sterile vacuum filter systems combine a filter funnel and receiving bottle, letting you sterilize or clarify media, buffers and supplements and store the filtrate without a transfer step. Five volumes from 50 mL to 1000 mL and three pore sizes fit both small batches and bulk preparation.
This listing is for NEST catalog number 347003: Vacuum Filter System with Receiving Bottle, 50 mL, 0.45 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1). The polyethersulfone (PES) membrane offers low protein binding with a high flow rate, and the cup is molded from virgin polystyrene with a 45 mm neck. PES is not for use with chloroform, esters, amides, strong acids or strong bases. Units are E-beam sterilized to SAL 10⁻⁶, individually bagged, made in a cGMP, ISO 13485 facility and lot tested free of pyrogens, cytotoxins, DNase, RNase and DNA. LIGHT LABS stocks the NEST Scientific line and ships quickly from our Englewood, Colorado team.
Key Features
- Fast Flow PES – high flow rates shorten filtration time for large media volumes
- Low Protein Binding – preserves growth factors and serum proteins in media
- Complete System – filter funnel and receiving bottle in one sterile unit
- Three Pore Sizes – 0.10 µm, 0.22 µm and 0.45 µm options
- Sterile and Tested – E-beam sterilized to SAL 10⁻⁶ and lot tested free of pyrogens and nucleases
Specifications
| Catalog Number | 347003 |
| Brand | NEST Scientific |
| Description | Vacuum Filter System with Receiving Bottle, 50 mL, 0.45 µm PES Membrane, Low Protein Binding, Sterile |
| Sold As | Case of 12 (12 x 1) |
| Volume | 50 mL |
| Pore Size | 0.45 µm |
| Sterile | Yes |
| Units per Pack | 1 |
| Units per Case | 12 |
| Membrane | PES (polyethersulfone) |
| Configuration | Filter funnel with receiving bottle |
| Housing | Virgin polystyrene cup with cap |
| Neck | 45 mm |
| Chemical Limits | Not for chloroform, esters, amides, strong acids or strong bases |
| Sterilization | E-beam, SAL 10⁻⁶ |
| Certified free of | Pyrogens (USP 85), cytotoxins (USP 87/88), DNase, RNase, DNA, BSE/TSE; animal-origin components |
Available Options
- 347002 – Vacuum Filter System with Receiving Bottle, 50 mL, 0.10 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 347001 – Vacuum Filter System with Receiving Bottle, 50 mL, 0.22 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 346002 – Vacuum Filter System with Receiving Bottle, 150 mL, 0.10 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 346001 – Vacuum Filter System with Receiving Bottle, 150 mL, 0.22 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 346003 – Vacuum Filter System with Receiving Bottle, 150 mL, 0.45 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 342002 – Vacuum Filter System with Receiving Bottle, 250 mL, 0.10 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 342001 – Vacuum Filter System with Receiving Bottle, 250 mL, 0.22 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 342003 – Vacuum Filter System with Receiving Bottle, 250 mL, 0.45 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 343002 – Vacuum Filter System with Receiving Bottle, 500 mL, 0.10 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 343001 – Vacuum Filter System with Receiving Bottle, 500 mL, 0.22 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 343003 – Vacuum Filter System with Receiving Bottle, 500 mL, 0.45 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 344002 – Vacuum Filter System with Receiving Bottle, 1000 mL, 0.10 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 344001 – Vacuum Filter System with Receiving Bottle, 1000 mL, 0.22 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
- 344003 – Vacuum Filter System with Receiving Bottle, 1000 mL, 0.45 µm PES Membrane, Low Protein Binding, Sterile, Case of 12 (12 x 1)
Applications
- Cell culture media sterilization
- Media clarification
- Buffer and reagent filtration
- Serum and supplement filtration
- Tissue culture prep
- Mycoplasma reduction (0.10 µm)
