Accuris SmartDye™ PR6000-100 Terminator v3.1 Cycle Sequencing Kit — 100 Reactions

Product Description

The Accuris SmartDye™ PR6000-100 is a 100-reaction Terminator v3.1 Cycle Sequencing Kit — a direct, economical replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit, requiring no protocol changes, no new mobility shift files, and no recalibration. Built on the proven Sanger sequencing method and optimized for longer, high-quality reads with minimal noise, SmartDye delivers exceptional accuracy even in notoriously difficult GC-rich regions. Supplied as a ready-to-use premix containing 800µL SmartDye v3.1 Sequencing Mix and 1mL 5× optimized buffer.

Direct Drop-In Replacement for BigDye™ Terminator v3.1 — No Protocol Changes Required SmartDye seamlessly replaces Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit with zero protocol adjustment. Simply substitute SmartDye for BigDye in your existing sequencing protocol — the same primer concentrations, template amounts, cycle parameters, and capillary electrophoresis analysis workflow all apply without modification. No new mobility shift files need to be loaded into your sequencing instrument software, and no recalibration is required. For labs currently using BigDye v3.1, switching to SmartDye is the lowest-friction cost reduction available in the sequencing workflow.

Faster Reaction Times — As Little as 3 Minutes SmartDye supports faster cycle sequencing reaction times — as little as 3 minutes — without affecting the quality or read length of results. This is a meaningful throughput improvement for high-volume sequencing labs running many reactions per day, where cycle time directly impacts daily output and instrument utilization.

Superior Performance on Difficult Templates — Including GC-Rich Regions SmartDye is engineered for robust performance on challenging templates, including those with high GC content, repeat sequences, and other problematic secondary structures that can cause premature termination, signal dropout, or noisy traces with other sequencing chemistries. The optimized dye chemistry yields a high signal-to-noise ratio, facilitating clear and accurate interpretation of sequencing data across a broad range of template types — outperforming BigDye v3.1 on difficult templates in direct side-by-side comparisons.

Flexible — Supports Multiple Sequencing Workflows and Template Types SmartDye supports all major Sanger sequencing workflows including de novo sequencing, resequencing, and finishing. Compatible with a wide variety of template types:

• PCR products
• Plasmids
• BACs (Bacterial Artificial Chromosomes)
• Fosmids
• Other standard Sanger sequencing templates

Cost-Effective — Use as Little as 0.15µL Per Reaction Research has demonstrated that SmartDye performs well in smaller reaction volumes — as little as 0.15µL in a 5µL total volume reaction — making it significantly more budget-friendly than standard BigDye protocols that call for larger volumes. Combined with the economical per-reaction pricing of the PR6000-100, SmartDye delivers substantial reagent cost savings without compromising sequencing quality.

What's Included — PR6000-100 Kit Contents

• 800µL SmartDye v3.1 Terminator Sequencing Mix (fluorescently labelled ddNTP terminators + DNA polymerase + optimized buffer components)
• 1mL 5× Sequencing Buffer

Complete SmartDye™ Terminator v3.1 Kit Family — All Pack Sizes:

Key Features

• Direct replacement for Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit
• No protocol adjustment, no new mobility shift files, no recalibration required
• Built on proven Sanger sequencing method
• Optimized for longer, high-quality reads with minimal noise
• High signal-to-noise ratio — clear, accurate trace interpretation
• Superior performance on GC-rich and difficult templates
• Faster cycle times — as little as 3 minutes
• Supports de novo sequencing, resequencing, and finishing workflows
• Compatible with PCR products, plasmids, BACs, fosmids, and standard templates
• Minimum reaction volume: 0.15µL in 5µL total — highly economical
• Ready-to-use premix — 5× buffer included
• 100 reactions (800µL dye + 1mL 5× buffer)
• Available in 24, 100, 1,000, 5,000, and 25,000 reaction packs

Supported Applications Sanger cycle sequencing, de novo DNA sequencing, resequencing and variant confirmation, sequencing finishing and gap closure, PCR product sequencing, plasmid sequencing, BAC and fosmid sequencing, GC-rich template sequencing, and any application currently using Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit.

Technical Specifications