Accuris SmartDye™ PR6000-25K Terminator v3.1 Cycle Sequencing Kit — 25,000 Reactions

The Accuris SmartDye™ PR6000-25K is a 25,000-reaction Terminator v3.1 Cycle Sequencing Kit — the largest and most cost-efficient pack size in the SmartDye line, designed for the highest-throughput sequencing operations including genome centers, large institutional core facilities, clinical sequencing laboratories, and contract research organizations running continuous production-scale Sanger sequencing. A direct, economical replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit, requiring no protocol changes, no new mobility shift files, and no recalibration. Supplied as 10 × 20mL SmartDye v3.1 Sequencing Mix and 12mL 5× optimized buffer — the ultimate bulk format for maximum reagent economy and uninterrupted large-scale sequencing production.

Direct Drop-In Replacement for BigDye™ Terminator v3.1 — No Protocol Changes Required SmartDye seamlessly replaces Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit with zero protocol adjustment. Simply substitute SmartDye for BigDye in your existing sequencing protocol — the same primer concentrations, template amounts, cycle parameters, and capillary electrophoresis analysis workflow all apply without modification. No new mobility shift files need to be loaded, and no instrument recalibration is required. At 25,000-reaction scale, this frictionless transition from BigDye to SmartDye delivers the greatest absolute cost savings of any pack size in the SmartDye line — with zero disruption to established workflows, instrumentation, or data analysis pipelines.

25,000-Reaction Scale — The Ultimate Bulk Format for Genome Centers and Production Labs The PR6000-25K is designed for the most demanding sequencing operations — genome sequencing centers, large academic core facilities, hospital clinical sequencing labs, and CROs with continuous high-volume Sanger sequencing workflows measured in the tens of thousands of reactions per month. Supplied as ten separate 20mL bottles of SmartDye v3.1 Sequencing Mix, each bottle providing approximately 2,500 reactions, the PR6000-25K allows active bottles to be rotated through while sealed bottles maintain full stability — ensuring uninterrupted reagent supply for continuous production environments without compromising reagent integrity.

Maximum Cost Efficiency — The Lowest Per-Reaction Cost in the SmartDye Line At 25,000 reactions, the PR6000-25K delivers the absolute lowest per-reaction reagent cost across the entire SmartDye product line. Combined with SmartDye's demonstrated ability to perform effectively in smaller reaction volumes — as little as 0.15µL in a 5µL total volume reaction — the PR6000-25K represents the most cost-efficient Sanger sequencing reagent investment available for production-scale laboratories. For genome centers, core facilities, and CROs where sequencing reagent spend is a major operational cost, the PR6000-25K provides the most substantial and sustainable cost reduction compared to BigDye v3.1 at equivalent volume.

Faster Reaction Times — As Little as 3 Minutes SmartDye supports faster cycle sequencing reaction times — as little as 3 minutes — without affecting the quality or read length of results. At 25,000-reaction scale, even modest improvements in cycle time translate to significant gains in daily throughput, instrument utilization, and operational efficiency — all critical metrics for production sequencing environments operating under customer turnaround time commitments and capacity constraints.

Superior Performance on Difficult Templates — Including GC-Rich Regions SmartDye is engineered for robust performance on challenging templates, including those with high GC content, repeat sequences, and other problematic secondary structures that cause premature termination, signal dropout, or noisy traces. The optimized dye chemistry yields a high signal-to-noise ratio for clear and accurate trace interpretation across the full diversity of template types encountered in high-throughput sequencing environments. At 25,000-reaction production scale, consistently superior results on difficult templates eliminates the compounding cost of failed runs, repeat sequencing, and per-sample troubleshooting that erodes productivity and profitability in large-volume operations.

Flexible — Supports Multiple Sequencing Workflows and Template Types SmartDye supports all major Sanger sequencing workflows including de novo sequencing, resequencing, and finishing. Compatible with a wide variety of template types:

• PCR products
• Plasmids
• BACs (Bacterial Artificial Chromosomes)
• Fosmids
• Other standard Sanger sequencing templates

What's Included — PR6000-25K Kit Contents

• 10 × 20mL SmartDye v3.1 Terminator Sequencing Mix (fluorescently labelled ddNTP terminators + DNA polymerase + optimized buffer components)
• 12mL 5× Sequencing Buffer

Complete SmartDye™ Terminator v3.1 Kit Family — All Pack Sizes:

Key Features

• Direct replacement for Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit
• No protocol adjustment, no new mobility shift files, no recalibration required
• 25,000-reaction pack — the largest and most economical SmartDye format
• Supplied in 10 × 20mL bottles for maximum stability and stock management flexibility
• Built on proven Sanger sequencing method
• Optimized for longer, high-quality reads with minimal noise
• High signal-to-noise ratio — clear, accurate trace interpretation
• Superior performance on GC-rich and difficult templates
• Faster cycle times — as little as 3 minutes
• Supports de novo sequencing, resequencing, and finishing workflows
• Compatible with PCR products, plasmids, BACs, fosmids, and standard templates
• Minimum reaction volume: 0.15µL in 5µL total — maximum reagent economy
• Lowest per-reaction cost across the entire SmartDye product line
• Ready-to-use premix — 5× buffer included

Supported Applications Production-scale and genome center Sanger cycle sequencing, large academic and institutional core facility sequencing services, clinical Sanger sequencing at high volume, CRO sequencing services, de novo DNA sequencing, resequencing and variant confirmation, sequencing finishing and gap closure, PCR product sequencing, plasmid sequencing, BAC and fosmid sequencing, GC-rich template sequencing, and any production-volume application currently using Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit at the largest possible scale.

Technical Specifications