Accuris SmartDye™ PR6000-5K Terminator v3.1 Cycle Sequencing Kit — 5,000 Reactions

Product Description

The Accuris SmartDye™ PR6000-5K is a 5,000-reaction Terminator v3.1 Cycle Sequencing Kit — the enterprise-scale pack size of the SmartDye line, purpose-built for high-throughput sequencing core facilities, genomics centers, contract research organizations, and institutional labs running thousands of Sanger sequencing reactions per month. A direct, economical replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit, requiring no protocol changes, no new mobility shift files, and no recalibration. Supplied as 2 × 20mL SmartDye v3.1 Sequencing Mix and 12mL 5× optimized buffer — sized for sustained, uninterrupted large-scale sequencing production.

Direct Drop-In Replacement for BigDye™ Terminator v3.1 — No Protocol Changes Required SmartDye seamlessly replaces Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit with zero protocol adjustment. Simply substitute SmartDye for BigDye in your existing sequencing protocol — the same primer concentrations, template amounts, cycle parameters, and capillary electrophoresis analysis workflow all apply without modification. No new mobility shift files need to be loaded, and no instrument recalibration is required. At 5,000-reaction scale, this frictionless transition from BigDye to SmartDye delivers the largest per-unit cost savings in the SmartDye product line while completely eliminating the risk and disruption typically associated with switching sequencing chemistry.

5,000-Reaction Scale — Enterprise and Core Facility Production The PR6000-5K is designed for organizations that sequence at production scale — sequencing cores servicing large research institutions, genomics companies, clinical laboratories, and CROs running continuous high-volume Sanger sequencing workflows. Supplied as two separate 20mL bottles of SmartDye v3.1 Sequencing Mix, the PR6000-5K provides approximately 2,500 reactions per bottle — allowing one bottle to remain sealed and stable while the other is in active use, maximizing reagent shelf life and minimizing waste in high-consumption environments.

Maximum Cost Efficiency — The Most Economical Pack Size Per Reaction At 5,000 reactions, the PR6000-5K delivers the lowest per-reaction reagent cost in the SmartDye line. Combined with SmartDye's demonstrated ability to perform well in smaller reaction volumes — as little as 0.15µL in a 5µL total volume reaction — the PR6000-5K represents the most cost-efficient Sanger sequencing reagent option available for high-volume labs. For core facilities and production environments where reagent spend is a major budget line item, the PR6000-5K provides meaningful, sustainable cost reduction without any compromise in sequencing quality or throughput.

Faster Reaction Times — As Little as 3 Minutes SmartDye supports faster cycle sequencing reaction times — as little as 3 minutes — without affecting the quality or read length of results. At 5,000-reaction scale, faster cycle times translate directly to greater daily throughput and improved instrument utilization — critical performance metrics for core facilities and production environments operating under tight turnaround time commitments.

Superior Performance on Difficult Templates — Including GC-Rich Regions SmartDye is engineered for robust performance on challenging templates, including those with high GC content, repeat sequences, and other problematic secondary structures that cause premature termination, signal dropout, or noisy traces with other sequencing chemistries. The optimized dye chemistry yields a high signal-to-noise ratio, facilitating clear and accurate trace interpretation across diverse template types. At 5,000-reaction production scale, consistently superior results on difficult templates directly reduces the financial and operational burden of failed runs, repeat sequencing, and sample-specific troubleshooting.

Flexible — Supports Multiple Sequencing Workflows and Template Types SmartDye supports all major Sanger sequencing workflows including de novo sequencing, resequencing, and finishing. Compatible with a wide variety of template types:

• PCR products
• Plasmids
• BACs (Bacterial Artificial Chromosomes)
• Fosmids
• Other standard Sanger sequencing templates

What's Included — PR6000-5K Kit Contents

• 2 × 20mL SmartDye v3.1 Terminator Sequencing Mix (fluorescently labelled ddNTP terminators + DNA polymerase + optimized buffer components)
• 12mL 5× Sequencing Buffer

Complete SmartDye™ Terminator v3.1 Kit Family — All Pack Sizes:

Key Features

• Direct replacement for Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit
• No protocol adjustment, no new mobility shift files, no recalibration required
• 5,000-reaction pack — enterprise and core facility production scale
• Supplied in 2 × 20mL bottles for optimal reagent stability management
• Built on proven Sanger sequencing method
• Optimized for longer, high-quality reads with minimal noise
• High signal-to-noise ratio — clear, accurate trace interpretation
• Superior performance on GC-rich and difficult templates
• Faster cycle times — as little as 3 minutes
• Supports de novo sequencing, resequencing, and finishing workflows
• Compatible with PCR products, plasmids, BACs, fosmids, and standard templates
• Minimum reaction volume: 0.15µL in 5µL total — maximum reagent economy
• Lowest per-reaction cost in the SmartDye line
• Ready-to-use premix — 5× buffer included

Supported Applications High-throughput production-scale Sanger cycle sequencing, sequencing core facility services, genomics center workflows, clinical Sanger sequencing, CRO sequencing services, de novo DNA sequencing, resequencing and variant confirmation, sequencing finishing and gap closure, PCR product sequencing, plasmid sequencing, BAC and fosmid sequencing, GC-rich template sequencing, and any production-volume application currently using Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit.

Technical Specifications