Accuris SmartDye™ PR6000-1K Terminator v3.1 Cycle Sequencing Kit — 1,000 Reactions

Product Description

The Accuris SmartDye™ PR6000-1K is a 1,000-reaction Terminator v3.1 Cycle Sequencing Kit — the high-throughput pack size of the SmartDye line, ideal for core sequencing facilities, high-volume research labs, and any laboratory running large numbers of Sanger sequencing reactions regularly. A direct, economical replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit, requiring no protocol changes, no new mobility shift files, and no recalibration. Supplied as 4 × 2mL SmartDye v3.1 Sequencing Mix and 12mL 5× optimized buffer — designed for maximum reagent stability and convenient large-volume workflow management.

Direct Drop-In Replacement for BigDye™ Terminator v3.1 — No Protocol Changes Required SmartDye seamlessly replaces Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit with zero protocol adjustment. Simply substitute SmartDye for BigDye in your existing sequencing protocol — the same primer concentrations, template amounts, cycle parameters, and capillary electrophoresis analysis workflow all apply without modification. No new mobility shift files need to be loaded, and no instrument recalibration is required. For core facilities and high-throughput labs currently running BigDye v3.1, switching to SmartDye PR6000-1K delivers immediate, meaningful reagent cost savings at scale without disrupting established protocols or instrument settings.

1,000-Reaction Scale — Designed for High-Volume and Core Facility Use The PR6000-1K is the pack size of choice for sequencing core facilities, genomics cores, contract research organizations, and high-volume research labs that run dozens to hundreds of sequencing reactions per day. Supplied in four separate 2mL aliquots of SmartDye v3.1 Sequencing Mix — each aliquot provides approximately 250 reactions — allowing labs to open only what they need while keeping the remaining stock sealed and stable. The 12mL 5× buffer supply is sized to match the 1,000-reaction volume, providing everything needed for a complete workflow without the need for multiple orders.

Faster Reaction Times — As Little as 3 Minutes SmartDye supports faster cycle sequencing reaction times — as little as 3 minutes — without affecting the quality or read length of results. At 1,000-reaction scale, this throughput improvement translates to significantly greater daily output and improved instrument utilization — a meaningful operational advantage for busy core facilities where cycle time directly determines how many samples can be processed per run and per day.

Superior Performance on Difficult Templates — Including GC-Rich Regions SmartDye is engineered for robust performance on challenging templates, including those with high GC content, repeat sequences, and other problematic secondary structures that can cause premature termination, signal dropout, or noisy traces with other sequencing chemistries. The optimized dye chemistry yields a high signal-to-noise ratio, facilitating clear and accurate trace interpretation — outperforming BigDye v3.1 on difficult templates in direct side-by-side comparisons. At 1,000-reaction scale, consistently superior results on difficult templates directly reduces the cost and time burden of failed or repeat sequencing runs.

Cost-Effective — Use as Little as 0.15µL Per Reaction Research has demonstrated that SmartDye performs well in smaller reaction volumes — as little as 0.15µL in a 5µL total volume reaction. At 1,000-reaction scale, the combination of economical per-reaction pricing and the ability to use smaller volumes than standard BigDye protocols delivers substantial reagent cost savings — making the PR6000-1K one of the most cost-efficient options available for high-volume Sanger sequencing.

Flexible — Supports Multiple Sequencing Workflows and Template Types SmartDye supports all major Sanger sequencing workflows including de novo sequencing, resequencing, and finishing. Compatible with a wide variety of template types:

• PCR products
• Plasmids
• BACs (Bacterial Artificial Chromosomes)
• Fosmids
• Other standard Sanger sequencing templates

What's Included — PR6000-1K Kit Contents

• 4 × 2mL SmartDye v3.1 Terminator Sequencing Mix (fluorescently labelled ddNTP terminators + DNA polymerase + optimized buffer components)
• 12mL 5× Sequencing Buffer

Complete SmartDye™ Terminator v3.1 Kit Family — All Pack Sizes:

Key Features

• Direct replacement for Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit
• No protocol adjustment, no new mobility shift files, no recalibration required
• 1,000-reaction pack — ideal for core facilities and high-throughput labs
• Supplied in 4 × 2mL aliquots for convenient stock management
• Built on proven Sanger sequencing method
• Optimized for longer, high-quality reads with minimal noise
• High signal-to-noise ratio — clear, accurate trace interpretation
• Superior performance on GC-rich and difficult templates
• Faster cycle times — as little as 3 minutes
• Supports de novo sequencing, resequencing, and finishing workflows
• Compatible with PCR products, plasmids, BACs, fosmids, and standard templates
• Minimum reaction volume: 0.15µL in 5µL total — maximizes reagent economy
• Ready-to-use premix — 5× buffer included

Supported Applications High-throughput Sanger cycle sequencing, core facility sequencing services, de novo DNA sequencing, resequencing and variant confirmation, sequencing finishing and gap closure, PCR product sequencing, plasmid sequencing, BAC and fosmid sequencing, GC-rich template sequencing, and any high-volume application currently using Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit.

Technical Specifications