Product Description
Complete, reliable bisulfite conversion with minimal DNA loss is the foundation of any methylation study. This original Zymo Research conversion kit streamlines treatment and clean-up so you can move confidently from genomic DNA to methylation arrays or sequencing libraries. It is a trusted choice for many labs.
This listing is for catalog number D5001: EZ DNA Methylation Kit, 50 Reactions. Sodium bisulfite converts unmethylated cytosine to uracil through a three-step reaction, while methylated cytosines remain unchanged. In-column desulphonation replaces cumbersome precipitations, and the workflow is designed to limit template degradation while ensuring full conversion. Converted DNA is suitable for NGS library prep, PCR, endonuclease digestion, sequencing and microarrays, and is recommended for Illumina Infinium MethylationEPIC BeadChips. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Complete conversion – Designed to fully convert unmethylated cytosines.
- Minimal DNA loss – Reduces template degradation during treatment and clean-up.
- No precipitations – Desulphonation performed on the column.
- Array recommended – Suitable for Illumina Infinium MethylationEPIC BeadChips.
- Flexible downstream use – Ready for NGS, PCR, sequencing and arrays.
Specifications
| Catalog Number | D5001 |
| Brand | Zymo Research |
| Description | EZ DNA Methylation Kit, 50 Reactions |
| Chemistry | Sodium bisulfite conversion of cytosine to uracil |
| Desulphonation | On-column |
| Recommended for | Illumina Infinium MethylationEPIC BeadChip |
| Pack sizes | 50 or 200 reactions |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- Methylation arrays
- Bisulfite sequencing and NGS library prep
- Methylation-specific PCR
- Restriction-based methylation analysis
- Epigenetic biomarker research
