Product Description
When turnaround matters, a bisulfite workflow that finishes in under an hour and a half keeps methylation projects moving. This kit pairs a ready-made conversion reagent with spin-column cleanup, removing reagent preparation from the process and delivering concentrated converted DNA that is ready for PCR, sequencing, or array analysis.
This listing is for catalog number D5030: EZ DNA Methylation-Lightning Kit, 50 Reactions. Add the Lightning Conversion Reagent directly to DNA and incubate; there are no powders to dissolve or solutions to mix. Converted DNA is then desulphonated and purified on a spin column, with elution possible in as little as 10 µl. Unmodified cytosines are converted to uracil with high efficiency. Kits are available in 10-, 50-, and 200-reaction sizes. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Fast workflow – Converts and purifies DNA in less than 1.5 hours.
- Ready-to-use reagent – Conversion reagent is added directly to the sample with no preparation.
- Low elution volume – Elute converted DNA in as little as 10 µl.
- High conversion efficiency – Unmodified cytosines are efficiently converted to uracil.
- Multiple sizes – Offered in 10-, 50-, and 200-reaction kits.
Specifications
| Catalog Number | D5030 |
| Brand | Zymo Research |
| Description | EZ DNA Methylation-Lightning Kit, 50 Reactions |
| Processing time | Less than 1.5 hours |
| Elution volume | As low as 10 µl |
| Format | Spin column |
| Kit sizes | 10 (D5030T), 50 (D5030), 200 (D5031) reactions |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
- D5030T – EZ DNA Methylation-Lightning Kit, 10 Reactions
- D5031 – EZ DNA Methylation-Lightning Kit, 200 Reactions
Applications
- Bisulfite sequencing
- Methylation-specific PCR
- Methylation arrays
- Targeted methylation NGS
- Pyrosequencing
