Product Description
Shorten bisulfite incubations by combining denaturation and conversion into one step. This refinement of the EZ DNA Methylation Kit uses heat rather than chemical denaturation, giving methylation researchers a faster route to converted DNA. It is well suited to labs that prioritize turnaround and want fewer hands-on steps.
This listing is for catalog number D5005: EZ DNA Methylation-Gold Kit, 50 Reactions. Because input DNA is heat-denatured, denaturation and bisulfite conversion happen together in a single incubation, which greatly reduces total reaction time. Converted DNA is then purified and desulphonated on a Zymo-Spin column. The recovered DNA is well suited to PCR amplification for endonuclease digestion, sequencing, microarrays and other methylation analyses. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Heat denaturation – Replaces chemical denaturation of input DNA.
- Single-step reaction – Denaturation and conversion combined.
- Shorter incubation – Substantially reduced reaction time.
- PCR ready – Converted DNA suited to amplification-based analyses.
Specifications
| Catalog Number | D5005 |
| Brand | Zymo Research |
| Description | EZ DNA Methylation-Gold Kit, 50 Reactions |
| Denaturation | Heat |
| Chemistry | Combined denaturation and bisulfite conversion |
| Pack sizes | 50 or 200 reactions |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- Methylation-specific PCR
- Bisulfite sequencing
- Methylation arrays
- Restriction-based methylation analysis
