Product Description
Skipping a separate DNA extraction saves time and preserves material when samples are scarce. This bisulfite kit converts DNA straight from blood, tissue, or cultured cells, delivering complete conversion with enough sensitivity to support amplification from as few as 10 cells or 50 pg of DNA.
This listing is for catalog number D5020: EZ DNA Methylation-Direct Kit, 50 Reactions. Samples are first digested with proteinase K, then treated with CT Conversion Reagent, which turns unmethylated cytosine into uracil while methylated cytosine remains unchanged. Desulphonation and cleanup follow on a spin column, yielding converted DNA ready for PCR, restriction digestion, sequencing, and microarrays. Purified DNA can also be used as input. The kit is offered in 50- and 200-reaction sizes. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- No prior extraction – Converts DNA directly from blood, tissue, and cells.
- High sensitivity – Supports amplification of converted DNA from as few as 10 cells or 50 pg DNA.
- Complete conversion – Designed for reliable, thorough bisulfite conversion.
- Broad downstream use – Converted DNA suits PCR, restriction digestion, sequencing, and arrays.
- Two kit sizes – Available in 50- and 200-reaction formats.
Specifications
| Catalog Number | D5020 |
| Brand | Zymo Research |
| Description | EZ DNA Methylation-Direct Kit, 50 Reactions |
| Sample types | Blood, tissue, cells, purified DNA |
| Minimum input | 10 cells or 50 pg DNA |
| Kit sizes | 50 reactions (D5020), 200 reactions (D5021) |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- Bisulfite sequencing
- Methylation-specific PCR
- Methylation microarrays
- Low-input and limited-sample methylation studies
- Restriction-based methylation analysis
