Product Description
Genome-wide methylation profiling does not always require bisulfite chemistry. This immunoprecipitation kit pulls methylated fragments out of fragmented genomic DNA within a few hours, enriching 5-methylcytosine-containing DNA more than one hundred-fold over unmethylated DNA so it can be analyzed by sequencing, arrays, or PCR in genome-wide studies.
This listing is for catalog number D5101: Methylated-DNA IP Kit, 10 Reactions. Fragmented genomic DNA is denatured and incubated with a highly specific anti-5-methylcytosine monoclonal antibody, and the resulting complexes are captured and washed before methylated DNA is recovered. Included control DNA and primers let you track the success of the assay. Enriched DNA works with PCR, bisulfite treatment, whole-genome amplification, deep sequencing, and microarrays. The kit contains 10 reactions. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Strong enrichment – Achieves over one hundred-fold enrichment of methylated versus non-methylated DNA.
- Specific antibody – Uses an anti-5-methylcytosine monoclonal antibody.
- Fast protocol – Immunoprecipitation is completed in a few hours.
- Built-in controls – Control DNA and primers monitor assay performance.
- Flexible downstream use – Recovered DNA suits PCR, sequencing, arrays, and bisulfite treatment.
Specifications
| Catalog Number | D5101 |
| Brand | Zymo Research |
| Description | Methylated-DNA IP Kit, 10 Reactions |
| Enrichment | Over 100-fold, methylated vs. non-methylated DNA |
| Antibody | Anti-5-Methylcytosine monoclonal |
| Time | A few hours |
| Input | Fragmented genomic DNA |
| Included controls | Control DNA and primers |
| Reactions | 10 |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Applications
- MeDIP-seq
- MeDIP microarray analysis
- Methylated region enrichment
- Genome-wide methylation screening
