Product Description
A truly unmodified reference sets the zero point for any cytosine modification assay. This DNA standard contains only unmodified cytosines, providing the baseline needed to calibrate methods that quantify 5-methylcytosine or 5-hydroxymethylcytosine and to confirm that background signal remains low. It pairs naturally with the 5-mC and 5-hmC standards in the same series.
This listing is for catalog number D5405-1: Cytosine DNA Standard, 2 µg. It is the unmodified member of the 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set and shares its sequence with the methylated and hydroxymethylated standards, so comparisons reflect modification alone. The standard is supplied as 2 µg and can be used on its own or alongside its modified counterparts. Use it as the zero-modification reference in your calibration series. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Unmodified reference – Contains 100% unmodified cytosines.
- Matched sequence – Shares its sequence with the 5-mC and 5-hmC standards.
- Calibration use – Defines the baseline for modification quantification.
- 2 µg supply – Provided as 2 µg of DNA.
Specifications
| Catalog Number | D5405-1 |
| Brand | Zymo Research |
| Description | Cytosine DNA Standard, 2 µg |
| Modification | 100% unmodified cytosine |
| Quantity | 2 µg |
| Set | Component of D5405 |
| Shipping Conditions | Ships on blue ice |
| Storage | -20°C |
| Note | Product contains multiple components shipped separately. Blue ice items and dry ice items must have separate ice boxes |
Applications
- Negative reference for 5-mC and 5-hmC assays
- Calibration curves
- Antibody specificity testing
