Product Description
Calibrating an assay for cytosine modifications is far more reliable when the only variable is the modification itself. This set provides three DNA standards with identical sequence that differ solely in their cytosine form, giving a clean basis for comparing and quantifying unmodified, methylated, and hydroxymethylated cytosine.
This listing is for catalog number D5405: 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set. Each standard is an 897 bp linear double-stranded DNA in which every cytosine is either unmodified, 5-methylcytosine, or 5-hydroxymethylcytosine. Because sequence and modification content are fully defined, the standards can be used individually or combined to calibrate antibody-based, enzymatic, sequencing, or mass spectrometry methods for quantifying cytosine modifications. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Three standards – Unmodified cytosine, 5-mC, and 5-hmC versions in one set.
- Identical sequence – All standards share the same sequence, isolating the modification.
- Complete modification – Each standard contains 100% of one cytosine form.
- Defined fragment – Linear dsDNA of 897 bp.
- Calibration ready – Suited to quantitative assays for cytosine modifications.
Specifications
| Catalog Number | D5405 |
| Brand | Zymo Research |
| Description | 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set |
| Standards | Cytosine, 5-methylcytosine, 5-hydroxymethylcytosine |
| Format | Linear dsDNA, 897 bp |
| Modification | 100% of cytosines in the stated form |
| Shipping Conditions | Ships on blue ice |
| Storage | -20°C |
Applications
- 5-mC and 5-hmC assay calibration
- Antibody specificity testing
- ELISA standard curves
- Mass spectrometry method validation
