Product Description
Measuring 5-hydroxymethylcytosine accurately requires a reference that carries the mark at a known level. This standard supplies DNA in which cytosines are present as 5-hydroxymethylcytosine, giving you a reliable positive reference for calibrating and validating assays that quantify this modification. It is useful for both calibration and specificity testing.
This listing is for catalog number D5405-3: 5-Hydroxymethylcytosine DNA Standard, 2 µg. It is the hydroxymethylated member of the 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set and shares its sequence with the unmodified and methylated standards, which makes it useful for checking whether a method can tell 5-hmC apart from 5-mC. The standard is supplied as 2 µg of DNA. Use it alongside its companion standards for full calibration. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Hydroxymethylated reference – Cytosines are present as 5-hydroxymethylcytosine.
- Matched sequence – Shares its sequence with the cytosine and 5-mC standards.
- Specificity checks – Helps confirm discrimination between 5-hmC and 5-mC.
- 2 µg supply – Provided as 2 µg of DNA.
Specifications
| Catalog Number | D5405-3 |
| Brand | Zymo Research |
| Description | 5-Hydroxymethylcytosine DNA Standard, 2 µg |
| Modification | 5-hydroxymethylcytosine |
| Quantity | 2 µg |
| Set | Component of D5405 |
| Shipping Conditions | Ships on blue ice |
| Storage | -20°C |
Applications
- 5-hmC assay calibration
- Quest 5-hmC ELISA positive reference
- Antibody specificity testing
- Hydroxymethylation method validation
