Product Description
Count gene expression across many samples without paying for full-length transcript coverage. This Zymo Research kit generates 3' mRNA sequencing libraries in roughly four hours from as little as 10 ng of total RNA, making large expression studies faster and more economical. It is well suited to studies with many samples.
This listing is for catalog number R3008: Zymo-Seq SwitchFree 3′ mRNA Library Kit, 12 Preps. SwitchFree technology ligates the P7 adapter directly to first-strand cDNA without template switching, simplifying reagent handling. Built-in unique molecular identifiers let you measure duplication and quantify expression accurately. Unique dual indexing primers and cleanup beads are included, and sample multiplexing scales for high-throughput gene counting. Offered in 12- and 96-prep sizes. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Fast workflow – complete libraries in about four hours.
- Low input – works with as little as 10 ng of total RNA.
- SwitchFree ligation – P7 adapter attaches directly to first-strand cDNA without template switching.
- Built-in UMIs – unique molecular identifiers support accurate duplicate assessment and quantification.
- All-in-one kit – includes unique dual indexing primers and magnetic cleanup beads.
Specifications
| Catalog Number | R3008 |
| Brand | Zymo Research |
| Description | Zymo-Seq SwitchFree 3′ mRNA Library Kit, 12 Preps |
| Library type | 3' mRNA-seq |
| Input | As little as 10 ng total RNA |
| Workflow time | About 4 hours |
| Molecular barcodes | Built-in UMIs |
| Indexing | Unique dual indexing primers included |
| Kit sizes | 12 or 96 preps |
| Shipping Conditions | Ships on dry ice |
| Storage | -80C, 4C |
| Note | Product contains multiple components shipped separately. (RT and Dry ice) |
Available Sizes & Components
Applications
- 3' mRNA-seq gene expression profiling
- High-throughput gene counting
- Drug and compound screening
- Differential expression studies
