Product Description
Profiling open chromatin from a modest number of cells no longer has to take days. This ATAC-seq library kit takes mammalian cells or tissue to indexed, Illumina-ready libraries in as little as four hours, starting from as few as 50,000 cells and requiring minimal hands-on time at the bench.
This listing is for catalog number D5458: Zymo-Seq ATAC Library Kit, 12 Reactions. A gentle lysis releases nuclei for collection while mitochondrial DNA largely stays behind in the discarded cell material. A transposase preloaded with Illumina adapters then fragments and tags accessible chromatin in one quick reaction. Tagmented DNA is indexed and amplified with the UDI Tag Primer Set, producing libraries ready for any Illumina sequencer. The kit provides 12 reactions. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Fast workflow – Produces libraries in as little as 4 hours.
- Low cell input – Starts from as few as 50,000 cells.
- Reduced mitochondrial carryover – Gentle lysis leaves mitochondrial DNA in the discarded fraction.
- One-step tagmentation – Preloaded transposase fragments and tags open chromatin together.
- Unique dual indexing – UDI Tag Primer Set supports multiplexed Illumina sequencing.
Specifications
| Catalog Number | D5458 |
| Brand | Zymo Research |
| Description | Zymo-Seq ATAC Library Kit, 12 Reactions |
| Minimum input | 50,000 cells |
| Time | As little as 4 hours |
| Sample types | Mammalian cells and tissue |
| Indexing | UDI Tag Primer Set |
| Platform | Illumina |
| Reactions | 12 |
| Shipping Conditions | Ships on dry ice |
| Storage | -80C/-20C/Room Temperature |
| Note | Product contains multiple components shipped separately |
Applications
- Chromatin accessibility profiling
- Regulatory element discovery
- Transcription factor footprinting
- Cell-type epigenomic comparisons
