Product Description
The Excel Scientific X-Pierce™ XPS-25 is the sterile version of the X-Pierce™ precut pierceable sealing film — an 89µm vinyl film with a precut "X" over every well that creates four self-resealing flaps for repeated robotic probe and pipette tip access without coring, adhesive fouling, or probe occlusion. Identical in construction and performance to the non-sterile XP-100, the XPS-25 adds sterility for workflows where biological contamination from the sealing film is a concern — including clinical sample processing, viral work, cell-based assays, and any aseptic multi-access workflow requiring sterile plate protection. Sterile product is packaged in bags of 50. 25 films per pack. Dimensions: 79.4 × 145.5 mm. Temperature range: -40°C to +90°C.
The Precut "X" — Self-Resealing Multi-Access Technology A precut "X" over each well creates four vinyl flaps that bend downward when pushed by a robotic probe or pipette tip — allowing sample access without coring or adhesive fouling. A thin adhesive layer and special hairline cuts separating the flaps prevent probes from contacting adhesive and becoming occluded. The flaps return to their original closed position after each probe withdrawal — maintaining contamination and evaporation protection between access events. This self-resealing behavior enables unlimited repeated well access without replacing the seal — the defining advantage of the X-Pierce™ design over standard pierceable seals.
Sterile Format — For Clinical, Viral, and Aseptic Multi-Access Workflows The XPS-25 is the sterile format of the X-Pierce™ line — the correct choice for any multi-access workflow where biological sterility of the sealing film is required. Applications that specifically require the sterile XPS-25 over the non-sterile XP-100 include clinical molecular diagnostic sample processing, COVID-19 and infectious disease sample testing, viral culture plate workflows, cell-based multi-step assays, and any regulated or aseptic environment where non-sterile consumables cannot be used in direct contact with plate contents.
XPS-25 vs. XP-100 — Choosing the Right X-Pierce™ Format:
| XPS-25 | XP-100 | |
|---|---|---|
| Sterility | ✅ Sterile | Non-sterile |
| Pack Size | 25 films | 100 films |
| Packaging | Bags of 25 (sterile) | Standard pack |
| Film Material | 89µm vinyl | 89µm vinyl |
| Adhesive | 18µm acrylic | 18µm acrylic |
| Precut X Per Well | ✅ Yes | ✅ Yes |
| Self-Resealing | ✅ Yes | ✅ Yes |
| Temp Range | -40°C to +90°C | -40°C to +90°C |
| Best For | Clinical, viral, aseptic workflows | General automation, HTS |
No Probe Coring — No Adhesive Fouling — No Probe Occlusion The thin 18µm adhesive layer and special hairline cuts separating the X-Pierce™ flaps combine to prevent probes from contacting adhesive and becoming occluded — eliminating the two most common and costly robotic liquid handling failures associated with standard pierceable seals. No adhesive transfer to probe surfaces, no adhesive carryover between wells, and no probe cleaning steps required between accesses.
⚠️ Temporary Short-Term Use Only — Apply a Second Seal for Long-Term Storage The X-Pierce™ XPS-25 is designed for short-term processing and temporary protection during active multi-access workflows. For long-term sealed storage after sampling is complete, apply a continuous sealing film (such as an AlumaSeal® or ThermalSeal® film) as a second layer over the X-Pierce™ film.
Printed Alignment Circles and Alphanumeric Well Labels Every X-Pierce™ film includes printed alignment circles for precise plate positioning and alphanumeric well labels (A1 through H12) — allowing easy visual identification of individual wells through the clear vinyl film during multi-access workflows.
Compatible Replacement — Cross-Reference Guide
The X-Pierce™ XPS-25 is a direct compatible replacement for sterile precut pierceable sealing films from several major manufacturers. Labs currently using any of the following products can switch directly to the XPS-25 without protocol changes:
- Thermo Fisher Scientific AB-0718 — Pre-Slit Sealing Film for robotic liquid handling, sterile format
- 4titude® 4ti-0540 — Pre-slit pierceable sealing film for automation, sterile
- Axygen® PCR-SX-100 — Pre-slit sealing film for robotic workstations, sterile format
- Brooks Life Sciences / FluidX — Sterile pre-slit vinyl sealing film for compound management
Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific plates and protocols.
Key Features
- Precut "X" over every well — four self-resealing flaps per well
- Sterile — for clinical, viral, and aseptic multi-access workflows
- No probe coring — no adhesive fouling — no probe occlusion
- Self-resealing after every access — continuous contamination and evaporation protection
- Designed for multiple robotic probe and pipette tip accesses per well
- 89µm clear vinyl film — visual sample monitoring
- 18µm thin adhesive layer — minimal adhesive contact during access
- Special hairline cuts prevent probe-to-adhesive contact
- Printed alignment circles for precise plate positioning
- Alphanumeric well labels (A1–H12) on film surface
- Temperature range: -40°C to +90°C
- ⚠️ For long-term storage after sampling — apply a continuous second seal
- Sterile — packaged in bags of 25
- Dimensions: 79.4 × 145.5 mm
- Film thickness: 89µm / Adhesive thickness: 18µm
- 25 films per pack
- Made in the USA
Supported Applications Sterile robotic liquid handling with repeated well access, clinical molecular diagnostic sample processing, COVID-19 and infectious disease sample testing in 96-well deep well plates, viral culture plate workflows, cell-based multi-step assays requiring sterile plate protection, aseptic automated compound management with multiple probe accesses, multi-step manual reagent addition in sterile workflows, and any 96-well plate workflow requiring sterile temporary contamination protection with repeated robotic or manual well access without seal removal.
Technical Specifications
| Specification | Value |
|---|---|
| SKU / Catalog # | XPS-25 |
| Manufacturer | Excel Scientific |
| Product Name | X-Pierce™ |
| Sterility | Sterile |
| Film Material | 89µm clear vinyl |
| Adhesive | 18µm acrylic (thin layer) |
| Well Access Design | Precut "X" per well — 4 self-resealing flaps |
| Self-Resealing | Yes — flaps return after probe withdrawal |
| Probe Coring | None |
| Probe Adhesive Contact | None |
| Temperature Range | -40°C to +90°C |
| Optical Properties | Clear |
| Well Labels | Alphanumeric (A1–H12) printed on film |
| Alignment Circles | Yes — printed on film |
| Plate Format | 96-well plates |
| Dimensions | 79.4 × 145.5 mm |
| Film Thickness | 89µm |
| Adhesive Thickness | 18µm |
| Long-Term Storage | Apply continuous second seal after sampling |
| Packaging | Bags of 25 — sterile |
| Quantity per Pack | 25 |
| Origin | Made in the USA |