Excel Scientific ThermalSeal® RTS™ TSS-RTQ-25 Optically Clear qPCR Sealing Film, Non-Sterile — 25/Pack

Product Description

The Excel Scientific ThermalSeal® RTS™ TSS-RTQ-25 is a non-sterile, optically clear microplate sealing film designed for real-time qPCR, sample storage, and protein crystallization applications — featuring the same proven 50µm inert encapsulated silicone adhesive technology as the full ThermalSeal® RTS™ line in a compact 25-film pack. The TSS-RTQ-25 is the ideal format for labs with lower sealing volume needs, evaluation purchases, and any workflow that does not require sterility — delivering the same exceptional optical clarity, chemical resistance, and seal strength as the larger pack sizes in the most accessible quantity available. Certified DNase-free, RNase-free, and nucleic acid-free. Dimensions: 76.2 × 133.4 mm.

Encapsulated Silicone Adhesive — The Strongest Available Seal, Inert Over Wells The defining technology of ThermalSeal® RTS™ is its encapsulated silicone adhesive system. The encapsulated silicone adhesive is non-tacky until pressed against the sealing surface, at which time adhesive is released only in sealing areas to form the strongest available heat-resistant seal around each well on the plate. Adhesive on non-sealing areas of the film, such as directly over sample wells, remains encapsulated and inert — eliminating any risk of adhesive-sample interaction, extractable contamination, or interference with fluorescence detection. This precision adhesive release mechanism is unique to ThermalSeal® RTS™ and is the primary reason the film forms a stronger, more consistent seal than standard pressure-sensitive adhesive films.

High Optical Clarity — Minimal Autofluorescence for Accurate qPCR Detection ThermalSeal® RTS™ films offer high optical clarity with minimal to no autofluorescence — critical for real-time qPCR and fluorescence-based detection where background signal from the sealing film directly affects assay sensitivity and result reproducibility. The TSS-RTQ-25 provides the same consistent, uniform optical clarity as the sterile TSS-RTQS-50 — making it fully suitable for qPCR, fluorescence imaging, and crystal detection applications that do not require sterility.

Non-Sterile Format — For Standard PCR, Storage, and Crystallization Workflows The TSS-RTQ-25 is the non-sterile format of the ThermalSeal® RTS™ line — appropriate for the majority of qPCR, sample storage, and protein crystallization workflows where biological sterility of the sealing film is not a requirement. For cell-based assays, viral work, and other workflows requiring sterile sealing materials, the TSS-RTQS-50 (sterile, 50/pack) is the appropriate choice.

25-Film Pack — Ideal for Evaluation and Lower-Volume Use The TSS-RTQ-25 is the smallest pack size in the ThermalSeal® RTS™ line — making it the natural choice for labs evaluating ThermalSeal® RTS™ for the first time, labs with low sealing volume needs, and any workflow where the 100-film pack (TSS-RTQ-100) would result in significant unused inventory. At 25 films, the TSS-RTQ-25 provides enough material to thoroughly evaluate the film across multiple plate formats and protocols before committing to a larger pack size.

Fits Within Raised Plate Rim — Prevents Film Lifting and Evaporation ThermalSeal® RTS™ films are sized to fit within the edges of raised-rim 96-well plates — preventing the film lifting and elevated evaporation rates that occur with films that overlap the plate rim. Two end tabs with perforated boundaries assist in precise film positioning on the plate, and clean tab removal after placement does not disturb the seal. This fit-within-rim design eliminates the most common cause of well-to-well evaporation variability in qPCR.

Non-Tacky Adhesive — Easy Handling, No Sticking to Gloves The non-tacky adhesive surface simplifies film handling before sealing — the film can be picked up, repositioned, and aligned on the plate without sticking to gloves or accumulating fingerprints that would compromise optical clarity. This is a significant advantage over tacky adhesive films that adhere immediately on contact and cannot be repositioned.

-80°C to +105°C Temperature Range — PCR, Cold Storage, and Everything Between The exceptional temperature range of -80°C to +105°C covers the full spectrum of microplate workflows — from ultra-low temperature sample storage at -80°C through qPCR thermal cycling at up to 105°C. A single film type covers every temperature stage of the workflow without requiring separate seal inventories.

Chemically Resistant — Including DMSO for HTS Applications The TSS-RTQ-25 is chemically inert with no extractables except at extreme pH, and is resistant to DMSO, acetonitrile, and methanol — suitable for compound library storage plates, high-throughput screening workflows, and any application involving chemically aggressive sample matrices.

Cross-Reference — Compatible Replacement For: The ThermalSeal® RTS™ film is widely used as a direct compatible replacement for the following products:

• Applied Biosystems® MicroAmp® Optical Adhesive Film (catalog # 4311971 / 4360954)
• Thermo Fisher Scientific MicroAmp® Optical Adhesive Film

TSS-RTQ-25 vs. TSS-RTQS-50 — Choosing the Right Format:

Key Features

• Optically clear 50µm polyolefin film
• 50µm inert encapsulated silicone adhesive — releases only at well-sealing zones
• Strongest available heat-resistant seal for evaporation prevention
• Adhesive remains inert over sample wells — no sample interference
• High optical clarity — minimal to no autofluorescence
• Fits within raised plate rim — prevents film lifting and evaporation
• Non-tacky adhesive — easy handling, no sticking to gloves
• Two end tabs with perforated boundaries for precise positioning
• Temperature range: -80°C to +105°C
• Chemically resistant — DMSO, acetonitrile, methanol
• Chemically inert — no extractables except at extreme pH
• Certified DNase-free, RNase-free, and nucleic acid-free
• Non-sterile
• Dimensions: 76.2 × 133.4 mm (with tabs); 76.2 × 113.0 mm (tabs removed)
• Shelf life: 36 months
• 25 films per pack
• Made in the USA

Supported Applications Real-time qPCR, endpoint PCR, sample storage at -80°C, protein crystallization, DMSO compound library storage (HTS), fluorescence-based microplate assays, and any 96-well plate workflow requiring optically clear, chemically resistant sealing with no autofluorescence interference where sterility is not required.

Technical Specifications