Falcon® 353108 | 25cm² Rectangular Canted Neck Cell Culture Flask with Vented Cap — Case of 60

Product Description

The Falcon® 353108 is the industry-standard T-25 rectangular canted neck cell culture flask — the starting point for most mammalian cell culture workflows and the most commonly used flask for cell line establishment, maintenance, and small-scale expansion. Featuring a 25cm² growth area, 70mL total volume, vacuum-gas plasma TC treatment for consistent cell attachment, and a vented screw cap with an integrated 0.2µm hydrophobic membrane for optimal gas exchange, the 353108 delivers the reliability and performance that has made the Falcon flask line the benchmark for cell culture worldwide. Sterilized by gamma irradiation. Nonpyrogenic (<0.1 EU/mL). Sold as a case of 60 individually wrapped flasks.

Vacuum-Gas Plasma TC Treatment — Consistent Cell Attachment Surface The 353108 features vacuum-gas plasma tissue culture treatment — a surface modification process that renders the polystyrene growth surface hydrophilic and negatively charged, promoting consistent cell attachment, spreading, and growth for all common anchorage-dependent cell lines. The uniformity of the plasma treatment is tightly controlled, delivering consistent performance lot-to-lot and flask-to-flask — critical for reproducible experiments and reliable cell banking.

T-25 Format — Ideal for Cell Line Establishment and Maintenance The 25cm² T-25 format is the workhorse of day-to-day cell culture. It provides enough surface area for routine passaging and maintenance of established cell lines, initial establishment of new cell lines from thawed stocks, small-scale protein expression, toxicity and viability assays, and any application where larger flask formats would result in unnecessary media consumption or excessive cell numbers. The T-25 is the natural starting point before scaling up to T-75 (353136) or T-150 (353112) formats.

Canted Neck Design — Easy Access and Secure Stacking The canted (angled) neck design provides two key advantages: clear, ergonomic pipette access to the growth surface at a comfortable working angle, and secure interlocking stacking in the incubator — reducing the risk of accidental spills and cross-contamination from tipped flasks on shared shelving.

Vented Cap with 0.2µm Hydrophobic Membrane The integrated 0.2µm hydrophobic membrane in the vented screw cap allows passive CO&sub2; and O&sub2; exchange between the flask interior and the incubator atmosphere — maintaining the correct gas balance for pH regulation without requiring loosened caps. The hydrophobic membrane cannot be wetted by aqueous media, ensuring sterility is maintained even if the flask is inverted or roughly handled.

Optically Clear Polystyrene — Direct Microscopic Monitoring Manufactured from optically clear virgin polystyrene, the 353108 allows direct observation of cell morphology, confluency, and health under an inverted microscope without disturbing the culture — essential for routine quality monitoring and documentation.

Molded Volumetric Graduations and Writing Patch Molded graduations along both sides of the flask provide quick, accurate volume reference during media changes. A frosted writing patch provides a labeling surface for cell line, passage number, date, and operator initials — supporting good cell culture practice and traceability.

T-25 vs. T-75 — Choosing the Right Flask Size:

Related Falcon® Cell Culture Flask Sizes:

Key Features

• 25cm² growth area (T-25) — industry standard for cell maintenance and small-scale culture
• Rectangular canted neck — easy pipette access, secure incubator stacking
• Vented screw cap with 0.2µm hydrophobic membrane — passive gas exchange, sterility maintained
• Vacuum-gas plasma TC treatment — consistent, uniform cell attachment
• Optically clear virgin polystyrene — direct microscopic observation
• Molded volumetric graduations
• Frosted writing patch for labeling
• Total volume: 70mL
• Sterilized by gamma irradiation
• Nonpyrogenic — <0.1 EU/mL
• Stable from 4°C to 60°C
• Case of 60 flasks, individually packaged

Supported Applications Anchorage-dependent cell line maintenance and passaging, cell line establishment from frozen stocks, small-scale viral vector production, toxicity and viability assays, protein expression from adherent cell lines, primary cell culture, stem cell culture, mycoplasma testing, and any mammalian cell culture application requiring a 25cm² growth surface.

Technical Specifications