Product Description
LIGHT LABS is proud to offer the CELLTREAT 25cm² Tissue Culture Treated Flask with Vent Cap (Cat # 229331) — a premium, sterile, TC-treated tissue culture flask designed for small-scale adherent mammalian cell culture applications requiring a step up in growth area from the T-12.5 (229321) format. With 25cm² of TC-treated growth area and a 3–7 mL working volume, the 229331 is the classic T-25 flask — one of the most widely used flask formats in cell biology labs worldwide — ideal for maintaining cell stocks, establishing new cultures, performing small-scale drug screening, or as a starter flask before scaling up to larger formats.
The tissue culture treated surface delivers a consistent, uniform hydrophilic surface optimized for maximum adherent cell attachment and spreading across the full 25cm² growth area — manufactured under class 100,000 cleanroom conditions using USP Class VI virgin polystyrene for exceptional consistency and reliability. The large opening canted neck design with moderate angles provides easy access to the entire flask surface — particularly important when using pipettes or cell scrapers. The stable flat base allows the flask to stand upright during filling. The green HDPE vent cap with 0.22µm hydrophobic membrane maintains sterility while allowing consistent gas exchange with the CO₂ incubator atmosphere. Every flask is printed with lot number and expiration date for quality traceability, and flasks are packaged in convenient zip-closure resealable bags. LIGHT LABS is your trusted source for CELLTREAT laboratory plasticware.
KEY FEATURES
- 25cm² TC-treated growth area — classic T-25 flask format, one of the most widely used sizes in cell biology research worldwide
- 3–7 mL working volume; 50 mL maximum volume
- Tissue culture treated (TC-treated) surface — uniform hydrophilic surface for consistent, maximum adherent cell attachment; negatively charged hydrophilic surface with flat, striation-free growth area
- Manufactured under class 100,000 cleanroom conditions using USP Class VI virgin polystyrene
- 100% flask integrity tested during manufacturing for leak-free reliability
- Vent cap with 0.22µm hydrophobic membrane — allows CO₂/gas exchange with incubator atmosphere while maintaining sterility; green HDPE cap for easy vent cap identification
- Large opening, canted neck design with moderate angles — easy access to entire flask surface with pipettes and cell scrapers
- Stable flat base — flask stands upright during filling without a stand or holder
- Thick, durable polystyrene walls for structural integrity
- Every flask printed with lot number and expiration date for easy identification and quality traceability
- Convenient zip-closure resealable bag packaging
- Sterile — gamma irradiated
- Certified nonpyrogenic and RNase-/DNase-free
- Packaged 10 flasks per resealable bag; 20 bags per case (200 flasks/case)
- Fisher Scientific Catalog No. 50-202-078
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | 229331 |
| Fisher Scientific Catalog No. | 50-202-078 |
| Brand | CELLTREAT Scientific Products |
| Product Name | 25cm² TC-Treated Tissue Culture Flask with Vent Cap |
| Growth Area | 25 cm² |
| Maximum Volume | 50 mL |
| Working Volume | 3–7 mL |
| Surface Treatment | Tissue Culture Treated (TC-Treated) |
| Cap Type | Vent Cap (0.22µm hydrophobic membrane) |
| Cap Color | Green HDPE |
| Neck Design | Canted neck, large opening, moderate angle |
| Base | Stable flat base (stands upright) |
| Graduated | Yes |
| Material | USP Class VI virgin polystyrene (clear); Green HDPE (cap) |
| Manufacturing Environment | Class 100,000 cleanroom |
| Integrity Testing | 100% tested |
| Sterility | Sterile (gamma irradiated) |
| Certifications | Nonpyrogenic, RNase-free, DNase-free |
| Lot/Expiry Printing | Yes — lot number and expiration date printed on each flask |
| Packaging | 10 flasks/resealable zip-closure bag; 20 bags/case |
| Quantity | 200 flasks/case |
APPLICATIONS
- Small-scale mammalian cell line maintenance and routine passaging
- Initial culture establishment from primary cells or thawed cryovials
- Small-scale drug screening and dose-response studies
- Single-cell cloning and colony isolation
- Cell stock maintenance for banking and archiving
- Small-scale viral production in adherent cell lines
- Transfection optimization at small scale before scaling up
- Teaching and training labs running routine cell culture workflows
- Any adherent cell culture application requiring a TC-treated T-25 format flask
