MTC Bio OptiWell™ D3096-29 | 96-Well Deep Well Plate, Round Flat Bottom, 1.2mL, Non-Sterile — 50/Case (10 Bags of 5)

The MTC Bio OptiWell™ D3096-29 is a 96-well deep well plate with round flat-bottom wells and 1.2mL capacity per well — non-sterile, virgin medical-grade polypropylene, black printed alpha-numerics, minimal binding and liquid retention, compatible with all popular automated research platforms and robotic equipment. OptiWell plates come in 24, 48, 96, or 384 format for virtually every research lab, HTS application, and automated/robotic platform. These plates are useful for bacterial cultures, centrifugation (up to 4,000 × g) and general sample storage. Plates are produced and packaged in a Class 100,000 clean room and are ANSI/SLAS 1-2004 compliant. All plates are certified non-pyrogenic and RNase/DNase free. Case of 50 plates — 10 bags of 5 plates each. The sterile version is available as D3096-29S.

D3096-29 vs. D3096-29S — Non-Sterile vs. Sterile: Choosing the Right Format The D3096-29 and D3096-29S are identical OptiWell™ 96-well round flat-bottom 1.2mL deep well plates — sterility is the sole difference:

The D3096-29 non-sterile format is the correct choice for labs that do not require validated sterility — including compound management, sample storage, automated liquid handling reformatting, absorbance and OD reading workflows, and any application where the plate contents do not require a sterile vessel. For bacterial culture, protein expression, and cell-based assays where sterility is required, the D3096-29S sterile format is the appropriate choice.

Round Flat-Bottom Wells — Optimal for Optical Detection and Absorbance Measurement The round flat-bottom well geometry of the D3096-29 is the preferred format for 96-well deep well applications requiring optical reading of well contents — including absorbance measurement, turbidity monitoring, fluorescence reading from above or below, and any workflow where the flat well base provides a consistent optical path through the sample. The flat bottom of each well creates a uniform liquid depth across the full well base area — ensuring consistent optical path length for all wells on the plate and eliminating the depth variation at the well base that affects absorbance readings in V-bottom and U-bottom formats. For applications such as bacterial growth monitoring by OD600, enzymatic assay kinetics at 1.2mL volume, and colorimetric assays in deep well format — where a sterility requirement is absent — the D3096-29 provides the same flat-bottom optical performance as the sterile D3096-29S at a lower per-plate cost.

1.2mL Per Well — Mid-Range Deep Well Volume for Sample and Compound Processing The 1.2mL per-well capacity of the D3096-29 sits between the standard 1.0mL format (D3096-15) and the large 2.2mL format — providing meaningful additional volume over standard microplates and 1.0mL deep well plates while maintaining a compact plate height. For compound library management, sample storage, automated liquid handling workflows, and any non-sterile application requiring more volume than a standard 96-well microplate without the full depth of a 2.2mL deep well plate, the D3096-29 is the optimal specification.

D3096-29 in the Full OptiWell™ 96-Well Deep Well Family:

Automated Platform Compatibility — ANSI/SLAS 1-2004 Standard For automated research platforms and robotic equipment. The D3096-29 conforms to ANSI/SLAS 1-2004 standard 96-well microplate footprint dimensions — ensuring compatibility with all major automated liquid handling platforms including Hamilton, Tecan, Beckman Coulter, PerkinElmer, Agilent, and other standard HTS robotic systems.

4,000 × g Centrifugation Rating Centrifugation up to 4,000 × g. The robust polypropylene construction of the D3096-29 withstands centrifugal forces up to 4,000 × g — covering all standard plate centrifugation protocols for sample concentration, pelleting, and processing at 1.2mL scale.

Virgin Medical-Grade Polypropylene — Minimal Binding and Liquid Retention Minimal binding / liquid retention. Virgin, medical-grade polypropylene. Manufactured from virgin medical-grade polypropylene — free from recycled resin contaminants, processing aids, and extractables. Certified RNase/DNase free and non-pyrogenic despite being non-sterile — the non-sterile designation refers to the absence of gamma irradiation sterilization only, not to the cleanliness or purity of the polypropylene resin.

Black Printed Alpha-Numerics — Instant Well Identification Black printed alpha-numerics. Molded black alpha-numeric well identification (A through H rows, 1 through 12 columns) allows instant identification of specific wells during liquid handling setup, plate reading, and data recording.

Class 100,000 Cleanroom Production — Certified Purity Plates are produced and packaged in a Class 100,000 clean room. All plates are certified non-pyrogenic and RNase/DNase free — maintaining molecular biology grade purity standards despite the non-sterile designation.

Compatible Replacement — Cross-Reference Guide

The MTC Bio OptiWell™ D3096-29 is a direct compatible replacement for non-sterile 96-well round flat-bottom 1.2mL deep well plates from several major manufacturers:

• Thermo Fisher AB-0859 — Thermo Scientific 96-well round flat-bottom deep well plate, 1.2mL, non-sterile
• Greiner Bio-One 780201 — 96-well deep well plate, round flat-bottom, 1.2mL, non-sterile
• Eppendorf® 0030503390 — Eppendorf 96-well deepwell plate, round flat-bottom, 1.2mL, non-sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Key Features

• MTC Bio OptiWell™ — Catalog # D3096-29
• 96-well deep well plate — round flat-bottom wells
• 1.2mL per-well capacity
• Round flat-bottom — optimal for optical detection, absorbance measurement, and OD reading
• Non-sterile — lower per-plate cost vs. sterile D3096-29S
• Virgin medical-grade polypropylene — minimal binding and liquid retention
• Black printed alpha-numerics — instant well identification
• ANSI/SLAS 1-2004 compliant — compatible with all standard automated liquid handling platforms
• 4,000 × g centrifugation rating
• Compatible with silicone sealing mats and sealing film
• No lid, no skirt
• Autoclavable — can be sterilized before use if required
• Class 100,000 cleanroom production
• Certified RNase-free and DNase-free
• Certified non-pyrogenic
• Sterile version available: D3096-29S
• Case of 50 plates — 10 bags of 5 plates

Supported Applications Compound library storage and management at 1.2mL scale, sample storage and archiving, automated liquid handling sample reformatting, absorbance and OD reading workflows not requiring sterile plates, colorimetric and turbidity assays in deep well format, enzymatic assay kinetics at mid-volume scale, HPLC sample preparation, automated compound dilution series, centrifugation workflows up to 4,000 × g, and any 96-well application requiring round flat-bottom deep well format at 1.2mL per-well capacity without a sterility requirement.

Technical Specifications