Product Description
Lysing E. coli should not mean sonicators, French presses or detergent-laden buffers. These Zymo Research XJa autolysis competent cells are engineered to break open on demand with one quick freeze-thaw, giving gentle, efficient and repeatable lysis across any number of samples. It is a practical option for protein and nucleic acid work.
This listing is for catalog number T3021: Mix & Go! Competent Cells - XJa Autolysis, 10 x 100 µl. During growth, cells mildly express a chromosomal bacteriophage lambda R gene encoding lambda endolysin. The cytoplasmic membrane shields the cell wall until harvest, after which a single brief freeze-thaw cycle triggers efficient lysis. The strain is also well suited to nucleic acid purification, and a DE3 version is offered separately for T7-driven protein expression. Supplied as Mix & Go! competent cells (10 x 100 µl) for simple transformation. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Built-in autolysis – a chromosomal bacteriophage lambda R gene (lambda endolysin) is mildly expressed during growth.
- One freeze-thaw lysis – a single brief freeze-thaw cycle disrupts the membrane and releases contents in minutes.
- Scales easily – process any number of samples without added time or labor, unlike sonication or French press.
- Detergent-free – compatible with a wide range of buffers and avoids potentially interfering lytic additives.
- Nucleic acid prep compatible – gentle lysis also supports DNA and RNA purification.
Specifications
| Catalog Number | T3021 |
| Brand | Zymo Research |
| Description | Mix & Go! Competent Cells - XJa Autolysis, 10 x 100 µl |
| Strain | E. coli XJa |
| Lysis mechanism | Chromosomal lambda R gene (lambda endolysin) with freeze-thaw trigger |
| Lysis time | Minutes |
| Format | Mix & Go! competent cells, 10 x 100 µl |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Applications
- Gentle E. coli lysis
- Recombinant protein extraction
- Plasmid and nucleic acid purification
- High-throughput lysis workflows
