Product Description
Express T7-driven proteins and release them without sonication. These Zymo Research XJa (DE3) autolysis competent cells combine a T7 expression host with built-in, freeze-thaw triggered lysis, making protein recovery fast, gentle and consistent from one sample to hundreds. It suits both single preps and screening.
This listing is for catalog number T3031: Mix & Go! Competent Cells - XJa(DE3) Autolysis, 10 x 100 µl. During growth, cells mildly express a chromosomal bacteriophage lambda R gene encoding lambda endolysin. The cytoplasmic membrane shields the cell wall until harvest, after which a single brief freeze-thaw cycle triggers efficient lysis. This strain carries a DE3 lysogen encoding T7 RNA polymerase, so it supports recombinant protein expression from T7 promoter-driven vectors. Supplied as Mix & Go! competent cells (10 x 100 µl) for simple transformation. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Built-in autolysis – a chromosomal bacteriophage lambda R gene (lambda endolysin) is mildly expressed during growth.
- One freeze-thaw lysis – a single brief freeze-thaw cycle disrupts the membrane and releases contents in minutes.
- Scales easily – process any number of samples without added time or labor, unlike sonication or French press.
- Detergent-free – compatible with a wide range of buffers and avoids potentially interfering lytic additives.
- T7 expression ready – DE3 lysogen encodes T7 RNA polymerase for T7 promoter-driven protein expression.
Specifications
| Catalog Number | T3031 |
| Brand | Zymo Research |
| Description | Mix & Go! Competent Cells - XJa(DE3) Autolysis, 10 x 100 µl |
| Strain | E. coli XJa (DE3) (DE3 lysogen, T7 RNA polymerase) |
| Lysis mechanism | Chromosomal lambda R gene (lambda endolysin) with freeze-thaw trigger |
| Lysis time | Minutes |
| Format | Mix & Go! competent cells, 10 x 100 µl |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Applications
- T7-driven recombinant protein expression
- Protein extraction without sonication
- High-throughput expression screening
- Nucleic acid purification from E. coli
