Product Description
Pair T7 protein expression with lysis that takes minutes rather than an hour at the sonicator. These Zymo Research XJb (DE3) autolysis competent cells break open after one quick freeze-thaw, simplifying protein recovery in buffers of your choice. That helps protein workflows scale up easily.
This listing is for catalog number T3051: Mix & Go! Competent Cells - XJb(DE3) Autolysis, 10 x 100 µl. During growth, cells mildly express a chromosomal bacteriophage lambda R gene encoding lambda endolysin. The cytoplasmic membrane shields the cell wall until harvest, after which a single brief freeze-thaw cycle triggers efficient lysis. This strain carries a DE3 lysogen encoding T7 RNA polymerase, so it supports recombinant protein expression from T7 promoter-driven vectors. Supplied as Mix & Go! competent cells (10 x 100 µl) for simple transformation. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Built-in autolysis – a chromosomal bacteriophage lambda R gene (lambda endolysin) is mildly expressed during growth.
- One freeze-thaw lysis – a single brief freeze-thaw cycle disrupts the membrane and releases contents in minutes.
- Scales easily – process any number of samples without added time or labor, unlike sonication or French press.
- Detergent-free – compatible with a wide range of buffers and avoids potentially interfering lytic additives.
- T7 expression ready – DE3 lysogen encodes T7 RNA polymerase for T7 promoter-driven protein expression.
Specifications
| Catalog Number | T3051 |
| Brand | Zymo Research |
| Description | Mix & Go! Competent Cells - XJb(DE3) Autolysis, 10 x 100 µl |
| Strain | E. coli XJb (DE3) (DE3 lysogen, T7 RNA polymerase) |
| Lysis mechanism | Chromosomal lambda R gene (lambda endolysin) with freeze-thaw trigger |
| Lysis time | Minutes |
| Format | Mix & Go! competent cells, 10 x 100 µl |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Applications
- T7-driven recombinant protein expression
- Protein extraction without sonication
- High-throughput expression screening
- Nucleic acid purification from E. coli
