Product Description
Simplify protein recovery from T7 expression cultures you grow yourself. This Zymo Research XJb (DE3) autolysis glycerol stock gives you cells that lyse in minutes after a single freeze-thaw, with no detergents and no added labor as sample numbers grow. It is a practical starting point for in-house expression work.
This listing is for catalog number T5051: XJb (DE3) Autolysis Glycerol Stock, 1 Tube. During growth, cells mildly express a chromosomal bacteriophage lambda R gene encoding lambda endolysin. The cytoplasmic membrane shields the cell wall until harvest, after which a single brief freeze-thaw cycle triggers efficient lysis. This strain carries a DE3 lysogen encoding T7 RNA polymerase, so it supports recombinant protein expression from T7 promoter-driven vectors. Supplied as a single glycerol stock tube for establishing your own cultures and competent cell preparations. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Built-in autolysis – a chromosomal bacteriophage lambda R gene (lambda endolysin) is mildly expressed during growth.
- One freeze-thaw lysis – a single brief freeze-thaw cycle disrupts the membrane and releases contents in minutes.
- Scales easily – process any number of samples without added time or labor, unlike sonication or French press.
- Detergent-free – compatible with a wide range of buffers and avoids potentially interfering lytic additives.
- T7 expression ready – DE3 lysogen encodes T7 RNA polymerase for T7 promoter-driven protein expression.
Specifications
| Catalog Number | T5051 |
| Brand | Zymo Research |
| Description | XJb (DE3) Autolysis Glycerol Stock, 1 Tube |
| Strain | E. coli XJb (DE3) (DE3 lysogen, T7 RNA polymerase) |
| Lysis mechanism | Chromosomal lambda R gene (lambda endolysin) with freeze-thaw trigger |
| Lysis time | Minutes |
| Format | Glycerol stock, 1 tube |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Applications
- T7-driven recombinant protein expression
- Protein extraction without sonication
- High-throughput expression screening
- Nucleic acid purification from E. coli
