Product Description
Maintain your own supply of self-lysing E. coli. This Zymo Research XJa autolysis glycerol stock lets you grow cells that break open with a single freeze-thaw, giving efficient, detergent-free lysis without sonication or French press equipment. It is a convenient starting point for in-house work.
This listing is for catalog number T5021: XJa Autolysis Glycerol Stock, 1 Tube. During growth, cells mildly express a chromosomal bacteriophage lambda R gene encoding lambda endolysin. The cytoplasmic membrane shields the cell wall until harvest, after which a single brief freeze-thaw cycle triggers efficient lysis. The strain is also well suited to nucleic acid purification, and a DE3 version is offered separately for T7-driven protein expression. Supplied as a single glycerol stock tube for establishing your own cultures and competent cell preparations. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Built-in autolysis – a chromosomal bacteriophage lambda R gene (lambda endolysin) is mildly expressed during growth.
- One freeze-thaw lysis – a single brief freeze-thaw cycle disrupts the membrane and releases contents in minutes.
- Scales easily – process any number of samples without added time or labor, unlike sonication or French press.
- Detergent-free – compatible with a wide range of buffers and avoids potentially interfering lytic additives.
- Nucleic acid prep compatible – gentle lysis also supports DNA and RNA purification.
Specifications
| Catalog Number | T5021 |
| Brand | Zymo Research |
| Description | XJa Autolysis Glycerol Stock, 1 Tube |
| Strain | E. coli XJa |
| Lysis mechanism | Chromosomal lambda R gene (lambda endolysin) with freeze-thaw trigger |
| Lysis time | Minutes |
| Format | Glycerol stock, 1 tube |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Applications
- Gentle E. coli lysis
- Recombinant protein extraction
- Plasmid and nucleic acid purification
- High-throughput lysis workflows
