Product Description
Start in-house cultures of a T7 expression host that lyses itself on cue. This Zymo Research XJa (DE3) autolysis glycerol stock delivers cells that release their contents after a single brief freeze-thaw, simplifying protein extraction across any number of samples. It suits labs that grow their own expression cultures.
This listing is for catalog number T5031: XJa (DE3) Autolysis Glycerol Stock, 1 Tube. During growth, cells mildly express a chromosomal bacteriophage lambda R gene encoding lambda endolysin. The cytoplasmic membrane shields the cell wall until harvest, after which a single brief freeze-thaw cycle triggers efficient lysis. This strain carries a DE3 lysogen encoding T7 RNA polymerase, so it supports recombinant protein expression from T7 promoter-driven vectors. Supplied as a single glycerol stock tube for establishing your own cultures and competent cell preparations. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Built-in autolysis – a chromosomal bacteriophage lambda R gene (lambda endolysin) is mildly expressed during growth.
- One freeze-thaw lysis – a single brief freeze-thaw cycle disrupts the membrane and releases contents in minutes.
- Scales easily – process any number of samples without added time or labor, unlike sonication or French press.
- Detergent-free – compatible with a wide range of buffers and avoids potentially interfering lytic additives.
- T7 expression ready – DE3 lysogen encodes T7 RNA polymerase for T7 promoter-driven protein expression.
Specifications
| Catalog Number | T5031 |
| Brand | Zymo Research |
| Description | XJa (DE3) Autolysis Glycerol Stock, 1 Tube |
| Strain | E. coli XJa (DE3) (DE3 lysogen, T7 RNA polymerase) |
| Lysis mechanism | Chromosomal lambda R gene (lambda endolysin) with freeze-thaw trigger |
| Lysis time | Minutes |
| Format | Glycerol stock, 1 tube |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Applications
- T7-driven recombinant protein expression
- Protein extraction without sonication
- High-throughput expression screening
- Nucleic acid purification from E. coli
