Product Description
LIGHT LABS is proud to offer the Zymo Research Direct-zol™ RNA MiniPrep Plus Kit (Cat # R2073) — the highest-volume, most complete format in the Direct-zol™ RNA MiniPrep Plus line, providing 200 preps with 200 mL of TRI Reagent™ packaged separately alongside the kit. The R2073 is the all-in-one solution for labs running high volumes of RNA isolation from TRI Reagent® or TRIzol®-preserved samples — providing both the reagent and the purification chemistry in a single order with the best per-prep economy in the Direct-zol™ MiniPrep Plus lineup.
The Direct-zol™ RNA MiniPrep Plus kit represents a fundamental leap forward in RNA isolation workflow efficiency — eliminating the most time-consuming, technically demanding steps of conventional TRIzol® RNA extraction: phase separation, chloroform extraction, and isopropanol precipitation. Instead, the Direct-zol™ method allows researchers to apply a TRI Reagent®-preserved sample directly to the Zymo-Spin™ IIICG Capped Column, then simply bind, wash, and elute — completing the entire RNA purification procedure in approximately 7 minutes. The result is high-purity total RNA (A260/A280 >1.8, A260/A230 >1.8) ready for RT-PCR, transcriptomics, microarray, sequencing, and other sensitive downstream applications.
A critical scientific advantage of the Direct-zol™ method is its unbiased recovery of small RNAs including miRNA. Conventional phase separation during TRIzol® extraction has been shown to selectively enrich for certain miRNA species over others — introducing significant bias into small RNA and miRNA profiling experiments. Because the Direct-zol™ method bypasses phase separation entirely, all RNA species ≥17 nucleotides are recovered uniformly and non-selectively — eliminating this bias and making the Direct-zol™ the method of choice for accurate miRNA and small RNA analysis. The included Zymo-Spin™ IIICG Capped Columns also include DNase I for optional on-column DNA digestion to produce strictly DNA-free RNA. LIGHT LABS is your trusted source for Zymo Research products.
KEY FEATURES
- 7-minute total RNA purification directly from TRI Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™, or similar acid-guanidinium-phenol reagents — no phase separation, precipitation, or chloroform steps required
- Unbiased miRNA recovery — bypasses phase separation which selectively enriches certain miRNA species; critical for accurate miRNA profiling and small RNA-seq
- Total RNA including small RNAs ≥17 nt — complete recovery of mRNA, rRNA, miRNA, siRNA, snRNA, and other small RNA species
- 200-prep format with 200 mL TRI Reagent™ included — highest-volume, best-economy format; everything needed for 200 preps of RNA isolation
- Zymo-Spin™ IIICG Capped Columns — up to 100 µg RNA binding capacity per column
- TRI Reagent® (included) inhibits RNase activity and inactivates viruses and other infectious agents — safe handling of infectious samples
- Compatible with TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™, and all similar acid-guanidinium-phenol reagents
- Compatible with samples containing chloroform, 1-bromo-3-chloropropane (BCP), or 4-bromoanisole
- DNase I included (lyophilized, 4 x 1500U) — on-column DNA digestion for strictly DNA-free RNA
- Wide sample compatibility: animal cells, tissue, bacteria, yeast, biological fluids, serum, plasma, blood, in vitro RNA products
- NGS-ready: ultra-pure RNA without phenol carryover; no DNA contamination when DNase I used
- RNA purity: A260/A280 >1.8; A260/A230 >1.8
- Genesee Scientific Catalog No. 11-331PBT
- Made in the USA by Zymo Research
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | R2073 |
| Genesee Scientific Catalog No. | 11-331PBT |
| Brand | Zymo Research |
| Kit Name | Direct-zol™ RNA MiniPrep Plus |
| Number of Preps | 200 |
| TRI Reagent™ Included | Yes — 200 mL (packaged separately) |
| RNA Binding Capacity | Up to 100 µg per Zymo-Spin™ IIICG Column |
| RNA Size Range | ≥17 nucleotides (total RNA including small RNAs/miRNA) |
| RNA Purity | A260/A280 >1.8; A260/A230 >1.8 |
| Processing Time | ~7 minutes |
| Phase Separation Required | No |
| Precipitation Required | No |
| miRNA Recovery | Unbiased — no phase separation bias |
| DNase I | Included — lyophilized, 4 x 1500U |
| DNA Digestion Buffer | 16 mL |
| Column Type | Zymo-Spin™ IIICG Capped Columns — 200 pcs |
| Input Compatibility | TRI Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and similar |
| Sample Compatibility | Cells, tissue, bacteria, yeast, biological fluids, serum, plasma, blood, in vitro RNA |
| Sample Inactivation | TRI Reagent® inactivates RNases, viruses, and other infectious agents |
| Storage Temperature | Room temperature |
| Kit Components | TRI Reagent™ 200 mL (packaged separately); Direct-zol™ RNA PreWash (160 mL concentrate); RNA Wash Buffer (48 mL concentrate); DNase I lyophilized (4 x 1500U); DNA Digestion Buffer (16 mL); DNase/RNase-Free Water (30 mL); Zymo-Spin™ IIICG Capped Columns (200); Collection Tubes (400); Instruction Manual |
APPLICATIONS
- Total RNA isolation from cells, tissue, bacteria, yeast, blood, serum, and plasma
- miRNA and small RNA profiling — unbiased recovery critical for accurate miRNA-seq
- RNA-seq and transcriptomics library preparation
- RT-PCR and qRT-PCR from TRIzol®-preserved samples
- Microarray hybridization
- Northern blotting
- Gene expression analysis
- Viral RNA isolation from biological fluids and infected cell cultures
- In vitro transcription product purification and concentration
- Any RNA isolation workflow from TRI Reagent®- or TRIzol®-preserved samples requiring maximum throughput at best per-prep economy
ORDERING INFORMATION — ZYMO RESEARCH DIRECT-ZOL™ RNA MINIPREP PLUS
| Cat # | Description | Preps | TRI Reagent™ Included |
|---|---|---|---|
| R2073 | Direct-zol™ RNA MiniPrep Plus, Zymo-Spin™ IIICG Capped Columns | 200 | Yes — 200 mL |
| R2072 | Direct-zol™ RNA MiniPrep Plus, Zymo-Spin™ IIICG Capped Columns | 200 | No |
| R2071 | Direct-zol™ RNA MiniPrep Plus, Zymo-Spin™ IIICG Capped Columns | 50 | Yes — 50 mL |
| R2070 | Direct-zol™ RNA MiniPrep Plus, Zymo-Spin™ IIICG Capped Columns | 50 | No |
| R2070T | Direct-zol™ RNA MiniPrep Plus, Zymo-Spin™ IIICG Capped Columns (Trial) | 10 | No |
| R1055 | Quick-RNA™ MiniPrep Kit | 200 | — |
| R1054 | Quick-RNA™ MiniPrep Kit | 50 | — |
| D7003 | Quick-DNA/RNA™ Miniprep Plus Kit (simultaneous DNA + RNA) | 50 | — |
