Zymo Research RNA Clean & Concentrator™-5 R1016 | 200 Preps, Without DNase I, Zymo-Spin™ IC Columns

The Zymo Research RNA Clean & Concentrator™-5 R1016 is a 200-preparation RNA cleanup and concentration kit — providing a simple and reliable method for the rapid preparation of high-quality, RT-PCR-ready RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (>17 nt), large RNAs (>200 nt), and/or small RNAs (17–200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations. DNase I Set is not included — for the DNase I-included version at 200 preps, see R1014. 200 preparations. Catalog # R1016. The 50-preparation version without DNase is available as R1015.

R1016 — The High-Volume No-DNase Format: Who Needs It The R1016 is the highest-throughput format in the RNA Clean & Concentrator™-5 family for labs that do not require in-column DNase treatment. At 200 preparations and without the DNase I Set, the R1016 is the preferred choice for:

• High-throughput IVT RNA cleanup — labs producing CRISPR guide RNAs, mRNA therapeutics, antisense oligonucleotides, or ribozymes by in vitro transcription at large scale, where DNase treatment is performed in a separate dedicated step
• High-volume cDNA cleanup — labs running large numbers of reverse transcription reactions that require cleanup and concentration of first-strand cDNA without DNase exposure
• Core facilities processing pre-DNased RNA — core genomics and transcriptomics facilities that receive RNA already confirmed DNA-free and need only cleanup and concentration at high throughput
• mRNA production facilities — facilities producing IVT mRNA for research or therapeutic applications where downstream processing includes a dedicated DNase step
• High-throughput small RNA workflows — labs processing large batches of miRNA, siRNA, or other small RNA species at scale

R1016 vs. R1015 — Choosing the Right Kit Size: The R1016 and R1015 are identical RNA Clean & Concentrator™-5 (no DNase) kits — the number of preparations and reagent volumes are the sole differences:

R1016 vs. R1014 — Without DNase vs. With DNase at 200 Preps:

Complete RNA Clean & Concentrator™-5 Family — Full Overview:

5-Minute RNA Cleanup — Ultra-Pure RNA at High Throughput Quick, 5-minute recovery of ultra-pure RNA (≥17 nt) from enzymatic reactions, aqueous phase (following TRIzol® extraction), and other sources. The 5-minute preparation time is preserved across all 200 preparations of the R1016 — allowing high-throughput labs and core facilities to process large batches of samples rapidly and consistently.

Selective RNA Recovery — Total RNA, Large RNA, or Small RNA Across 200 Preps The RNA Clean & Concentrator™-5 allows selective recovery of different RNA size classes from the same sample — available consistently across all 200 preparations of the R1016:

• Total RNA (>17 nt) — standard protocol recovers all RNA species
• Large RNA only (>200 nt) — modified ethanol ratio selectively retains mRNA and rRNA
• Small RNA only (17–200 nt) — save the flow-through to collect miRNA and siRNA

≥6µL Minimum Elution — Maximum Concentration at Scale The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥6 µl of RNase-free water — producing highly concentrated RNA from every one of the 200 preparations in the R1016 kit.

NGS-Ready RNA — A260/A280 and A260/A230 >1.8 Purity — A260/A280 & A260/A230 >1.8. RNA is ready for Next-Gen Sequencing, RT-qPCR, etc. — maintained consistently across all 200 preparations.

Broad Sample Compatibility Compatible with:

• TRIzol®/chloroform extraction aqueous phase — most common RCC cleanup input
• In vitro transcription (IVT) products — CRISPR guide RNA, mRNA, antisense RNA
• cDNA following reverse transcription — cleanup without DNase exposure
• Enzymatic reactions — removal of enzymes, primers, nucleotides, and salts
• Dilute RNA samples — concentration into small, analysis-ready volumes
• Compatible with ≤0.1% Sarkosyl, ≤0.1% SDS, ≤90% Sucrose, ≤90% Formamide, and ≤2% Formaldehyde

Room Temperature Storage — All Kit Components Store all kit components at room temperature — simplifying large-format kit storage in high-throughput lab environments.

Complete Kit Contents — R1016 (200 Preps):

Compatible Replacement — Cross-Reference Guide

The Zymo Research RNA Clean & Concentrator™-5 R1016 is a comparable alternative to high-volume RNA cleanup kits (without DNase) from several major manufacturers:

• Qiagen RNeasy MinElute Cleanup Kit (74204) — RNA cleanup and concentration kit
• New England Biolabs Monarch RNA Cleanup Kit (T2030L) — RNA cleanup without DNase
• Norgen Biotek RNA Cleanup and Concentration Kit (23600) — RNA cleanup kit
• Thermo Fisher RNA Clean XP (Beckman Coulter A63987) — SPRI bead-based RNA cleanup

These products may differ in protocol, yield, and performance characteristics. The R1016 is specifically cited in published literature and protocols by catalog number.

Key Features

• Zymo Research RNA Clean & Concentrator™-5 — Catalog # R1016
• 200 preparations with Zymo-Spin™ IC Columns — largest no-DNase format
• ⚠️ DNase I Set NOT included — use R1014 for in-column DNase treatment at 200 preps
• 5-minute cleanup procedure
• Single-buffer system — simple, rapid, reliable
• Selective recovery of total RNA (>17 nt), large RNA (>200 nt), or small RNA (17–200 nt)
• 10µg RNA binding capacity per column
• Minimum elution volume: ≥6µL
• A260/A280 and A260/A230 >1.8 — NGS-ready purity
• Ideal for high-volume IVT cleanup, cDNA cleanup, pre-DNased RNA processing at scale
• Compatible with TRIzol® aqueous phase, IVT products, cDNA, enzymatic reactions
• RT-PCR-ready, NGS-ready, hybridization-ready
• Room temperature storage — all kit components
• 1-year kit component integrity guarantee
• Lot-to-lot tested for performance and reliability
• Lowest per-prep cost in the no-DNase RNA Clean & Concentrator™-5 line
• Smaller pack size without DNase: R1015 (50 preps)
• With-DNase versions: R1013 (50 prep), R1014 (200 prep)

Supported Applications High-volume IVT product cleanup (CRISPR guide RNA, mRNA, antisense RNA, ribozymes), large-scale cDNA cleanup and concentration following reverse transcription, high-throughput RNA cleanup of pre-DNased samples, RNA cleanup and concentration following TRIzol®/phenol-chloroform extraction at scale, dilute RNA concentration, high-throughput small RNA and miRNA cleanup, RNA cleanup prior to NGS library preparation at scale, RT-qPCR sample preparation in large batches, mRNA therapeutic production cleanup, core facility RNA processing workflows, and any high-throughput molecular biology workflow requiring rapid, highly concentrated RNA with NGS-grade purity where DNase treatment is not required.

Technical Specifications