Zymo Research RNA Clean & Concentrator™-5 R1014 | 200 Preps with DNase I Set, Zymo-Spin™ IC Columns

The Zymo Research RNA Clean & Concentrator™-5 R1014 is a 200-preparation RNA cleanup and concentration kit — providing a simple and reliable method for the rapid preparation of high-quality, RT-PCR-ready, DNA-free RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (>17 nt), large RNAs (>200 nt), and/or small RNAs (17–200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations. DNase I Set included. 200 preparations. Catalog # R1014. The 50-preparation version is available as R1013.

R1014 vs. R1013 — Choosing the Right Kit Size: The R1014 and R1013 are identical RNA Clean & Concentrator™-5 kits in every way — the number of preparations and reagent volumes are the sole differences:

The R1014 is the preferred choice for high-throughput RNA biology labs, core genomics facilities, and any environment where RNA cleanup is a routine, high-frequency workflow step. At 200 preparations, the R1014 reduces kit reordering frequency, provides a lower per-prep reagent cost, and minimizes workflow interruption from kit changeovers.

R1014 vs. R1013 vs. R1015 vs. R1016 — Understanding the Complete Family:

The R1014 is the correct choice for high-volume labs that routinely require DNA-free RNA — the included DNase I Set allows in-column DNase digestion to eliminate genomic DNA contamination as an integrated single-workflow step across all 200 preparations.

5-Minute RNA Cleanup — Ultra-Pure RNA in Minutes at Scale Quick, 5-minute recovery of ultra-pure RNA (≥17 nt) from enzymatic reactions, aqueous phase (following TRIzol® extraction), and other sources. The 5-minute preparation time is preserved across the full 200-preparation R1014 kit — allowing high-throughput labs to process large batches of samples rapidly without sacrificing cleanup quality or RNA purity. For core facilities and labs running 10, 20, or more samples simultaneously, the consistent 5-minute protocol allows accurate throughput scheduling and capacity planning across the full kit lifetime.

DNase I Set Included — In-Column DNA Elimination Across All 200 Preps The R1014 is supplied with the DNase I Set (E1010) — enabling optional in-column DNase digestion to eliminate genomic DNA contamination directly on the column during the cleanup workflow across all 200 preparations. The in-column DNase treatment is performed directly on the spin column matrix between the RNA binding and wash steps — adding a single additional incubation step without requiring a separate tube, additional centrifugation steps, or heat inactivation that could affect RNA integrity.

Selective RNA Recovery — Total RNA, Large RNA, or Small RNA at High Throughput A distinctive feature of the RNA Clean & Concentrator™-5 is its ability to selectively recover different RNA size classes from the same sample — available across all 200 preparations of the R1014:

• Total RNA (>17 nt) — standard protocol recovers all RNA species including mRNA, rRNA, tRNA, and small RNAs
• Large RNA only (>200 nt) — modified protocol selectively retains mRNA and rRNA while small RNAs pass through
• Small RNA only (17–200 nt) — save the column flow-through from the large RNA protocol to collect miRNA, siRNA, and other small RNA species

≥6µL Minimum Elution — Highly Concentrated RNA in Minimal Volume The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥6 µl of RNase-free water — producing the most highly concentrated RNA eluate available from a spin column cleanup kit, maintained consistently across all 200 preparations of the R1014.

NGS-Ready RNA — A260/A280 and A260/A230 >1.8 Purity — A260/A280 & A260/A230 >1.8. RNA is ready for Next-Gen Sequencing, RT-qPCR, etc. The consistently high purity of RNA purified with the RNA Clean & Concentrator™-5 meets the purity standards required for next-generation sequencing library preparation — maintained across all 200 preparations in the R1014.

Broad Sample Compatibility — RNA from Any Source Compatible with:

• TRIzol®/chloroform extraction aqueous phase — the most common input for RCC cleanup
• In vitro transcription (IVT) reactions — CRISPR guide RNA, mRNA therapeutics, antisense RNA
• DNase I treated RNA — post-DNase cleanup
• Reverse transcription reactions — cDNA cleanup and concentration
• Enzymatic reactions — removal of enzymes, primers, nucleotides, and salts
• Dilute RNA samples — concentration into small, analysis-ready volumes
• Compatible with ≤0.1% Sarkosyl, ≤0.1% SDS, ≤90% Sucrose, ≤90% Formamide, and ≤2% Formaldehyde

Room Temperature Storage — Complete Kit at Bench Temperature Store all kit components at room temperature. All R1014 components are stored at room temperature — simplifying large-format kit storage in high-throughput lab environments. Reconstituted DNase I is stored as frozen aliquots after preparation.

Complete Kit Contents — R1014 (200 Preps):

Compatible Replacement — Cross-Reference Guide

The Zymo Research RNA Clean & Concentrator™-5 R1014 is a comparable alternative to high-volume RNA cleanup kits from several major manufacturers:

• Qiagen RNeasy MinElute Cleanup Kit (74204) — RNA cleanup and concentration kit
• Thermo Fisher RNA Clean XP (Beckman Coulter A63987) — SPRI bead-based RNA cleanup
• Norgen Biotek RNA Cleanup and Concentration Kit (23600) — RNA cleanup kit
• New England Biolabs Monarch RNA Cleanup Kit (T2030L) — RNA cleanup and concentration

These products may differ in protocol, yield, and performance characteristics. The R1014 is specifically cited in published literature and protocols by catalog number.

Key Features

• Zymo Research RNA Clean & Concentrator™-5 — Catalog # R1014
• 200 preparations with Zymo-Spin™ IC Columns — high-volume format
• DNase I Set (E1010) included — in-column DNA elimination across all 200 preps
• 5-minute cleanup procedure
• Single-buffer system — simple, rapid, reliable
• Selective recovery of total RNA (>17 nt), large RNA (>200 nt), or small RNA (17–200 nt)
• 10µg RNA binding capacity per column
• Minimum elution volume: ≥6µL
• A260/A280 and A260/A230 >1.8 — NGS-ready purity
• Compatible with TRIzol® aqueous phase, IVT products, DNase-treated RNA, enzymatic reactions
• RT-PCR-ready, NGS-ready, hybridization-ready
• Room temperature storage — all kit components
• 1-year kit component integrity guarantee
• Lot-to-lot tested for performance and reliability
• Lowest per-prep cost in the R1013/R1014 DNase-included line
• Smaller pack size available: R1013 (50 preps)
• Without DNase versions: R1015 (50 prep), R1016 (200 prep)

Supported Applications RNA cleanup and concentration following TRIzol®/phenol-chloroform extraction, in vitro transcription (IVT) product cleanup for CRISPR guide RNA, mRNA therapeutics and antisense RNA, post-DNase treatment RNA cleanup, cDNA cleanup following reverse transcription, dilute RNA concentration, small RNA and miRNA cleanup, RNA cleanup prior to NGS library preparation, RT-qPCR sample preparation, microarray hybridization, Northern blotting, RNA size fractionation (large vs. small RNA separation), high-throughput RNA processing in core genomics facilities, and any molecular biology workflow requiring rapid, highly concentrated, DNA-free RNA with NGS-grade purity at high volume.

Technical Specifications