Zymo Research Quick-DNA™ MicroPrep Kit D3021 | 200 Preps with Zymo-Spin™ IC Columns (Capped)

Product Description

The Zymo Research Quick-DNA™ MicroPrep Kit D3021 is a 200-preparation spin column DNA purification kit — ideal for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications. Supplied with Zymo-Spin™ IC Columns (Capped). 200 preparations. Catalog # D3021. The 50-preparation version is available as D3020. 

D3021 vs. D3020 — 200 Preps vs. 50 Preps:

Broad Sample Compatibility — From Whole Blood to Cultured Cells Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. The Quick-DNA™ MicroPrep Kit is designed for biological fluid and cell-based samples — including: 

• Whole blood (fresh or stored) — typically yields 1.5–3.5µg DNA per 50µL blood
• Buffy coat — concentrated leukocyte fraction from blood
• Buccal cells — non-invasive swab-based sampling
• Cells from culture — mammalian cell lines and primary cells
• Saliva — direct processing without pretreatment
• Plasma and serum — for circulating DNA
• Other biological liquids — urine, CSF, and compatible biological fluids

For solid tissue samples requiring Proteinase K digestion, the Quick-DNA™ MicroPrep Plus Kit (D4074) is the recommended upgrade.

Up to 5µg DNA Per Prep — High Yield in Minimal Elution Volume Up to 5µg total DNA is eluted into ≥10µl (6µl minimum) DNA Elution Buffer or water. Human whole blood will typically yield 1.5–3.5µg DNA per 50µl blood sampled. Mammalian tissues already homogenized yield 1–3µg DNA per mg skeletal, heart, and brain tissues. The minimal elution volume of ≥10µL (6µL minimum) produces a highly concentrated DNA eluate — ideal for applications requiring maximum DNA concentration in a small volume, including single-cell genotyping, limited-input sequencing library preparation, and sensitive PCR assays. 

Zymo-Spin™ IC Columns — High-Quality DNA in Minutes Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. The Zymo-Spin™ IC Column silica membrane selectively binds double-stranded DNA while allowing RNA to flow through — eliminating the need for RNase A treatment. The result is RNA-free genomic DNA eluted in a small, concentrated volume, ready for immediate use in downstream molecular biology applications without further cleanup or concentration. 

RNA-Free Genomic DNA — No RNase A Treatment Required The selective chemistry of the Zymo-Spin™ IC Column allows binding of double-stranded DNA while RNA passes through the column — producing RNA-free genomic DNA without RNase A treatment. This simplifies the purification workflow, reduces reagent costs, and eliminates the potential for RNase A carryover into the eluate that could affect RNA-containing downstream applications such as combined DNA/RNA analysis.

PCR Inhibitor Removal — Eluate Ready for Sensitive Downstream Applications PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications. The Genomic Lysis Buffer and wash buffer system of the Quick-DNA™ MicroPrep Kit effectively removes heme, IgG, and other PCR inhibitors commonly found in blood and biological fluid samples — producing a clean eluate that performs reliably in even the most sensitive PCR, qPCR, and sequencing applications without requiring additional cleanup steps. 

DNA Size — High Molecular Weight Fragments Up to and >40kb Quick purification of high quality DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, swabs or cultured cells in less than 15 mins using Zymo-Spin column technology. DNA size: up to and >40kb. The high molecular weight DNA recovered by the Quick-DNA™ MicroPrep Kit is suitable for applications requiring intact genomic DNA — including restriction endonuclease digestion, Southern blotting, RFLP analysis, microsatellite analysis, and next-generation sequencing library preparation. 

Room Temperature Storage — No Cold Chain Required for Buffers All kit components including Genomic Lysis Buffer, DNA Pre-Wash Buffer, g-DNA Wash Buffer, and DNA Elution Buffer are stored at room temperature — simplifying kit storage, eliminating cold chain requirements for the reagent components, and making the kit practical for field-based or resource-limited settings. Purified DNA is stored at ≤-20°C.

Extensively Referenced in Published Research The Zymo Research D3021 Quick-DNA™ MicroPrep Kit is one of the most widely cited DNA extraction kits in published molecular biology research — referenced in thousands of peer-reviewed publications across genomics, epigenetics, infectious disease, cancer biology, and clinical research. Genomic DNA was extracted from about 1 million cells using Quick-DNA Microprep Kit (ZYMO RESEARCH, D3021). 1µL of the genomic DNA was used as PCR template for genotyping using Phusion High-Fidelity DNA Polymerase. When a published protocol specifies D3021 or Quick-DNA MicroPrep Kit, this is the correct product. 

Complete Kit Contents — D3021 (200 Preps):

Compatible Replacement — Cross-Reference Guide

The Zymo Research Quick-DNA™ MicroPrep Kit D3021 is a reference standard in the genomic DNA extraction kit category. Researchers evaluating alternatives may also consider:

• Qiagen QIAamp DNA Blood Mini Kit (51106) — 50-prep blood DNA extraction kit
• Thermo Fisher PureLink Genomic DNA Mini Kit (K182001) — genomic DNA purification from blood and cells
• Promega ReliaPrep Blood gDNA Miniprep System (A5081) — blood and cell gDNA extraction

These products may differ in protocol, yield, and performance characteristics. The D3021 is specifically cited in published literature and protocols by catalog number.

Key Features

• Zymo Research Quick-DNA™ MicroPrep Kit — Catalog # D3021
• 200 preparations with Zymo-Spin™ IC Columns (Capped)
• Rapid isolation of total DNA — genomic, mitochondrial, and viral
• Broad sample compatibility — whole blood, buffy coat, buccal cells, cultured cells, saliva, plasma, serum, and other biological fluids
• Up to 5µg total DNA per preparation
• DNA yield from whole blood: 1.5–3.5µg per 50µL blood
• Minimum elution volume: ≥10µL (6µL minimum)
• High molecular weight DNA — up to and >40kb
• RNA-free — no RNase A treatment required
• PCR inhibitors effectively removed
• Purification complete in minutes
• Eluted DNA ideal for PCR, qPCR, DNA sequencing, restriction digestion, bisulfite conversion/methylation analysis, and nucleotide blotting
• Room temperature buffer storage — no cold chain for reagents
• Extensively cited in published research literature
• Smaller pack size available: D3020 (50 preps)
• Fisher Scientific catalog # 50-444-148

Supported Applications Genomic DNA extraction from whole blood, buffy coat, buccal swabs, cultured mammalian cells, saliva, plasma, serum, and biological fluids — for use in PCR, quantitative PCR (qPCR), Sanger sequencing, next-generation sequencing (NGS) library preparation, restriction endonuclease digestion, Southern blotting, RFLP analysis, microsatellite genotyping, bisulfite conversion and DNA methylation analysis, CRISPR genome editing genotyping, pharmacogenomics, infectious disease DNA detection, forensic applications, and any molecular biology workflow requiring rapid, high-quality genomic DNA purification from biological fluid or cell-based samples.

Technical Specifications