Zymo Research DNA Clean & Concentrator™-5 D4014 | 200 Preps with Zymo-Spin™ IC Columns (Capped)

The Zymo Research DNA Clean & Concentrator™-5 D4014 is the 200-preparation, high-volume PCR purification and DNA cleanup kit with capped Zymo-Spin™ IC Columns — providing purification of up to 5µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, and other sources. Clean and concentrate up to 5µg DNA with ≥6µL elution in as little as 2 minutes with 0µL wash residue carryover. Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer. Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc. Supplied with capped Zymo-Spin™ IC Columns. 200 preparations. Catalog # D4014. The 50-prep capped version is D4013. The uncapped column versions are D4003 (50 preps) and D4004 (200 preps).

D4014 vs. D4004 — Capped vs. Uncapped at 200 Preps: The Only Difference The D4014 and D4004 are identical DNA Clean & Concentrator™-5 kits in every way — the column cap is the sole difference:

The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.

D4014 vs. D4013 — Choosing the Right Kit Size: The D4014 and D4013 are identical capped DCC-5 kits — the number of preparations and reagent volumes are the sole differences:

Complete DCC-5 Family — All Catalog Numbers:

Why Choose Capped Columns — The Advantages of the Zymo-Spin™ IC The capped Zymo-Spin™ IC Column of the D4014 provides several practical workflow advantages over uncapped columns — particularly valuable in high-throughput environments where 200 preps are consumed regularly:

• Easy labeling — the flat cap surface provides a dedicated writing area for sample identification directly on the column — especially useful when processing many columns simultaneously and needing to track individual samples
• Added security — the cap prevents accidental column tip contamination during handling, transport between centrifuge and biosafety cabinet, and incubation steps
• Rack stability — capped columns stand upright in standard tube racks without tipping — particularly useful when processing large batches of columns simultaneously
• Elution incubation — the cap allows the elution buffer-loaded column to be inverted briefly if needed to ensure complete matrix hydration before centrifugation

2-Minute DNA Cleanup — The Fastest Spin Column Kit at High Volume Clean and concentrate up to 5µg DNA with ≥6µL elution in as little as 2 minutes with 0µL wash residue carryover. The 2-minute protocol is maintained across all 200 preparations of the D4014 — allowing high-throughput labs to process large batches rapidly and consistently regardless of cap format preference.

≥6µL Minimum Elution — Maximum DNA Concentration The ≥6µL minimum elution volume of the DCC-5 produces the most highly concentrated DNA eluate available from a standard PCR cleanup kit — maintained across all 200 preparations of the D4014. For NGS library preparation, Sanger sequencing, transfection, and single-cell workflows where maximum DNA concentration in minimal volume is critical, the DCC-5 capped column format delivers equivalent concentration performance to the uncapped format.

Zero Buffer Retention — No Wash Residue Carryover Zymo-Spin™ columns are designed for complete elution with no buffer retention or carryover. The zero buffer retention property is maintained in the capped IC column format — eliminating residual wash buffer carryover and downstream enzymatic inhibition across all 200 preparations.

Up to 5µg DNA Per Prep — 70–95% Recovery from 50bp to 10kb ≤5µg total DNA can be recovered. For DNA 50bp to 10kb the recovery is 70–95%. For DNA 11kb to 23kb the recovery is 50–70%. Recovery performance is identical between capped and uncapped DCC-5 column formats — maintained consistently across all 200 preparations of the D4014.

High Purity DNA — A260/A280 ≥1.8 Across All 200 Preps High-quality DNA (A260/A280 ≥1.8) ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures — maintained consistently across all 200 preparations.

Broad Sample Compatibility The D4014 facilitates removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Compatible sources include PCR reactions, restriction digests, modification reactions, NGS library prep steps, bisulfite conversion cleanup, and isotope/fluorescence labeling reactions.

Room Temperature Storage — All Kit Components All kit components stored at room temperature — simplifying storage of the larger D4014 reagent volumes. Note: Ethanol must be added to DNA Wash Buffer concentrate prior to use as indicated on the label.

Complete Kit Contents — D4014 (200 Preps):

Compatible Replacement — Cross-Reference Guide

The Zymo Research DNA Clean & Concentrator™-5 D4014 is a comparable alternative to high-volume PCR purification kits from several major manufacturers:

• Qiagen QIAquick PCR Purification Kit (28106) — 250-prep PCR cleanup kit, 30µL minimum elution
• Thermo Fisher GeneJET PCR Purification Kit (K0702) — 250-prep PCR cleanup kit
• NEB Monarch PCR & DNA Cleanup Kit (T1030L) — large-format PCR cleanup kit
• Macherey-Nagel NucleoSpin Gel and PCR Clean-up (740609.250) — 250-prep PCR cleanup kit

The D4014 differentiates with ≥6µL minimum elution, 2-minute protocol, and zero buffer retention across all 200 preparations. These products may differ in protocol, yield, and performance characteristics.

Key Features

• Zymo Research DNA Clean & Concentrator™-5 — Catalog # D4014
• 200 preparations with Zymo-Spin™ IC Columns (Capped) — high-volume format
• Up to 5µg total DNA per prep
• ≥6µL minimum elution volume — maximum DNA concentration
• 2-minute cleanup procedure — fastest spin column DNA cleanup available
• Zero buffer retention — no wash residue carryover, no dry spin required
• DNA recovery: 70–95% for 50bp–10kb; 50–70% for 11kb–23kb
• DNA size range: ~50bp to 23kb
• A260/A280 ≥1.8 — high-purity DNA across all 200 preparations
• Capped columns — easy labeling, added security, rack stability
• Room temperature storage — all kit components
• Ethanol must be added to DNA Wash Buffer concentrate before use
• 1-year kit component integrity guarantee
• Lot-to-lot tested for performance and reliability
• Lowest per-prep cost in the capped DCC-5 line
• 50-prep capped version: D4013
• Uncapped versions: D4003 (50 preps), D4004 (200 preps)

Supported Applications High-volume PCR product cleanup and desalting, large-batch restriction endonuclease digest purification, high-throughput NGS library preparation cleanup, core facility DNA cleanup services, high-throughput Sanger sequencing template preparation, DNA modification reaction cleanup at scale, isotope and fluorescence label removal, bisulfite conversion cleanup, CRISPR donor DNA preparation at scale, transfection-grade PCR product preparation in large batches, and any high-throughput molecular biology workflow requiring rapid, highly concentrated, ultra-pure DNA in 2 minutes per prep with capped column format.

Technical Specifications