Zymo Research Quick-DNA™ Miniprep Plus Kit D4069 | 200 Preps with Zymo-Spin™ IIC-XLR Columns, Proteinase K Included

The Zymo Research Quick-DNA™ Miniprep Plus Kit D4069 is the 200-preparation universal genomic DNA extraction kit — the high-volume format in the Quick-DNA™ Miniprep Plus line, designed for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (>50 kb) to be eluted in as little as 35 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free, removing the need for RNase A treatments and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis. Supplied with Zymo-Spin™ IIC-XLR Columns, Proteinase K & Storage Buffer (4×20mg), BioFluid & Cell Buffer (Red, 45mL), and Solid Tissue Buffer (Blue, 22mL). 200 preparations. Catalog # D4069. The 50-preparation version is available as D4068.

D4069 vs. D4068 — Choosing the Right Kit Size: The D4069 and D4068 are identical Quick-DNA™ Miniprep Plus Kits in every way — the number of preparations and reagent volumes are the sole differences:

The D4069 is the preferred choice for high-throughput genomics labs, core DNA extraction facilities, and any environment where the Quick-DNA™ Miniprep Plus Kit is a routine, high-frequency workflow tool — providing the lowest per-prep reagent cost in the Quick-DNA™ Miniprep Plus line and minimizing kit changeover frequency.

Universal Sample Compatibility — From Solid Tissue to Biological Fluids at Scale The Quick-DNA™ Miniprep Plus Kit processes the widest possible range of biological sample types — maintained across all 200 preparations of the D4069:

• Whole blood (fresh or stored) — 3–7µg DNA per 100µL blood
• Buffy coat — concentrated leukocyte fraction
• Saliva and buccal cells — non-invasive sampling at high throughput
• Solid tissue — up to 25mg per prep using Proteinase K and Solid Tissue Buffer (Blue)
• FFPE samples — formalin-fixed paraffin-embedded tissue
• Cultured cells — mammalian, insect, HeLa, HEK-293, buccal cells
• E. coli and bacteria — prokaryotic genomic DNA
• Sputum, semen, and other biological fluids
• Samples on storage papers/cards — Guthrie, FTA®, other storage cards
• Avian blood — nucleated blood samples
• Viral DNA from serum or plasma

Proteinase K Included — 4×20mg for 200 Preparations The D4069 includes 4 individual 20mg vials of lyophilized Proteinase K — each reconstituted separately to maintain freshness and activity across the full 200-preparation kit lifetime. The 4-vial format ensures that Proteinase K activity is preserved throughout extended kit use — preventing the activity loss that would occur if a single large vial were reconstituted at kit opening and used across all 200 preparations.

Two Color-Coded Lysis Buffers — Visual Confirmation at High Throughput The color-coded BioFluid & Cell Buffer (Red) and Solid Tissue Buffer (Blue) system is maintained across the full 200-preparation D4069 kit — providing the same unambiguous visual quality control at high throughput. For core facilities and high-volume labs processing multiple sample types simultaneously, the color-coded buffer system is especially valuable for preventing cross-use of the wrong buffer for a given sample type during high-pressure batch processing sessions.

Zymo-Spin™ IIC-XLR Column — 25µg Capacity, No Buffer Retention Across 200 Preps The 25µg DNA binding capacity and zero buffer retention of the Zymo-Spin™ IIC-XLR Column are maintained consistently across all 200 preparations of the D4069 — ensuring reliable, reproducible high-yield DNA extraction from every sample type throughout the full kit lifetime.

>50kb High Molecular Weight DNA — 3x More DNA at High Volume Ultra-pure and concentrated genomic DNA (>50 kb) eluted in as little as 35 µl. Recover 3x more DNA. The high molecular weight DNA and concentrated elution performance of the Quick-DNA™ Miniprep Plus Kit are maintained across all 200 preparations — providing consistent, high-quality genomic DNA for long-read sequencing, NGS library preparation, and other demanding downstream applications at the throughput scale that the D4069 enables.

RNA-Free DNA — No RNase A Treatment Across 200 Preps Purified DNA is RNA-free, removing the need for RNase A treatments and ensuring accurate quantification for applications like library preparations. The RNA-free property is maintained consistently across all 200 preparations of the D4069 — critical for high-throughput NGS library preparation workflows where RNA contamination would affect library quality metrics and sequencing results at scale.

20-Minute Procedure — Maintained at High Volume Simple 20-minute procedure. The streamlined protocol completes in approximately 20 minutes per prep — maintained across all 200 preparations of the D4069. For high-volume labs and core facilities processing many samples per day, the consistent 20-minute prep time allows accurate throughput scheduling and capacity planning across the full kit lifetime.

Room Temperature Buffer Storage Most kit components stored at room temperature — BioFluid & Cell Buffer (Red), Solid Tissue Buffer (Blue), Genomic Binding Buffer, DNA Pre-Wash Buffer, g-DNA Wash Buffer, DNA Elution Buffer, Zymo-Spin™ IIC-XLR Columns, and Collection Tubes. Proteinase K & Storage Buffer stored at −20°C after reconstitution.

Complete Kit Contents — D4069 (200 Preps):

Compatible Replacement — Cross-Reference Guide

The Zymo Research Quick-DNA™ Miniprep Plus Kit D4069 is a comparable alternative to high-volume universal genomic DNA extraction kits from several major manufacturers:

• Qiagen DNeasy Blood & Tissue Kit (69506) — 250-prep universal DNA extraction from blood, tissue, cells
• Thermo Fisher PureLink Genomic DNA Mini Kit (K182002) — genomic DNA from blood, tissue, cells
• Promega ReliaPrep gDNA Tissue Miniprep System (A2053) — high-volume tissue and cell DNA extraction
• NEB Monarch Genomic DNA Purification Kit (T3010L) — universal genomic DNA extraction

The D4069 provides 200 preparations with the broadest sample type compatibility including FFPE, storage cards, and avian blood. These products may differ in protocol, yield, and performance characteristics.

Key Features

• Zymo Research Quick-DNA™ Miniprep Plus Kit — Catalog # D4069
• 200 preparations with Zymo-Spin™ IIC-XLR Columns — high-volume format
• Universal sample compatibility — biological fluids, solid tissue, cells, FFPE, bacteria, blood cards
• Proteinase K included — 4×20mg for maintained activity across all 200 preparations
• Color-coded lysis buffers — BioFluid & Cell Buffer (Red), Solid Tissue Buffer (Blue)
• 25µg DNA binding capacity per column
• DNA yield from whole blood: 3–7µg per 100µL
• Tissue yields: 1–3µg per mg (skeletal, heart, lung, brain); 3–5µg per mg (liver, kidney)
• >50kb high molecular weight DNA
• Minimum elution volume: ≥35µL
• RNA-free — no RNase A treatment required
• No buffer retention — Zymo-Spin columns
• 20-minute procedure
• Recover 3× more DNA vs. standard kits
• Ready for qPCR, NGS, DNA-seq, arrays, methylation analysis
• Room temperature buffer storage (Proteinase K at −20°C after reconstitution)
• Lowest per-prep cost in the Quick-DNA™ Miniprep Plus line
• 1-year kit component integrity guarantee
• 50-prep version: D4068

Supported Applications High-throughput genomic DNA extraction from whole blood, buffy coat, solid tissue, FFPE samples, cultured mammalian cells, E. coli and bacteria, saliva, buccal cells, sputum, semen, blood storage cards (Guthrie, FTA®), avian blood, and viral DNA from serum and plasma — for use in PCR, qPCR, Sanger sequencing, next-generation sequencing (NGS) library preparation, long-read sequencing, restriction endonuclease digestion, Southern blotting, RFLP analysis, microsatellite genotyping, bisulfite conversion and DNA methylation analysis, CRISPR genome editing genotyping, pharmacogenomics, microarray analysis, and any high-throughput molecular biology workflow requiring high-yield, high molecular weight, RNA-free genomic DNA from the broadest possible range of sample types in 20 minutes per prep.

Technical Specifications