Product Description
The Zymo Research Quick-DNA™ Miniprep Plus Kit D4069 is the 200-preparation universal genomic DNA extraction kit — the high-volume format in the Quick-DNA™ Miniprep Plus line, designed for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (>50 kb) to be eluted in as little as 35 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free, removing the need for RNase A treatments and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis. Supplied with Zymo-Spin™ IIC-XLR Columns, Proteinase K & Storage Buffer (4×20mg), BioFluid & Cell Buffer (Red, 45mL), and Solid Tissue Buffer (Blue, 22mL). 200 preparations. Catalog # D4069. The 50-preparation version is available as D4068.
D4069 vs. D4068 — Choosing the Right Kit Size: The D4069 and D4068 are identical Quick-DNA™ Miniprep Plus Kits in every way — the number of preparations and reagent volumes are the sole differences:
| D4069 | D4068 | |
|---|---|---|
| Preparations | 200 | 50 |
| Proteinase K | 4×20mg | 20mg |
| BioFluid & Cell Buffer (Red) | 45mL | 12mL |
| Solid Tissue Buffer (Blue) | 22mL | 6mL |
| Genomic Binding Buffer | 85mL | 25mL |
| DNA Pre-Wash Buffer | 2×50mL | 30mL |
| g-DNA Wash Buffer | 200mL | 50mL |
| DNA Elution Buffer | 50mL | 10mL |
| Zymo-Spin™ IIC-XLR Columns | 200 | 50 |
| Collection Tubes | 400 | 100 |
| Best For | High-volume labs, core facilities | Standard labs, evaluation |
The D4069 is the preferred choice for high-throughput genomics labs, core DNA extraction facilities, and any environment where the Quick-DNA™ Miniprep Plus Kit is a routine, high-frequency workflow tool — providing the lowest per-prep reagent cost in the Quick-DNA™ Miniprep Plus line and minimizing kit changeover frequency.
Universal Sample Compatibility — From Solid Tissue to Biological Fluids at Scale The Quick-DNA™ Miniprep Plus Kit processes the widest possible range of biological sample types — maintained across all 200 preparations of the D4069:
- Whole blood (fresh or stored) — 3–7µg DNA per 100µL blood
- Buffy coat — concentrated leukocyte fraction
- Saliva and buccal cells — non-invasive sampling at high throughput
- Solid tissue — up to 25mg per prep using Proteinase K and Solid Tissue Buffer (Blue)
- FFPE samples — formalin-fixed paraffin-embedded tissue
- Cultured cells — mammalian, insect, HeLa, HEK-293, buccal cells
- E. coli and bacteria — prokaryotic genomic DNA
- Sputum, semen, and other biological fluids
- Samples on storage papers/cards — Guthrie, FTA®, other storage cards
- Avian blood — nucleated blood samples
- Viral DNA from serum or plasma
Proteinase K Included — 4×20mg for 200 Preparations The D4069 includes 4 individual 20mg vials of lyophilized Proteinase K — each reconstituted separately to maintain freshness and activity across the full 200-preparation kit lifetime. The 4-vial format ensures that Proteinase K activity is preserved throughout extended kit use — preventing the activity loss that would occur if a single large vial were reconstituted at kit opening and used across all 200 preparations.
Two Color-Coded Lysis Buffers — Visual Confirmation at High Throughput The color-coded BioFluid & Cell Buffer (Red) and Solid Tissue Buffer (Blue) system is maintained across the full 200-preparation D4069 kit — providing the same unambiguous visual quality control at high throughput. For core facilities and high-volume labs processing multiple sample types simultaneously, the color-coded buffer system is especially valuable for preventing cross-use of the wrong buffer for a given sample type during high-pressure batch processing sessions.
Zymo-Spin™ IIC-XLR Column — 25µg Capacity, No Buffer Retention Across 200 Preps The 25µg DNA binding capacity and zero buffer retention of the Zymo-Spin™ IIC-XLR Column are maintained consistently across all 200 preparations of the D4069 — ensuring reliable, reproducible high-yield DNA extraction from every sample type throughout the full kit lifetime.
>50kb High Molecular Weight DNA — 3x More DNA at High Volume Ultra-pure and concentrated genomic DNA (>50 kb) eluted in as little as 35 µl. Recover 3x more DNA. The high molecular weight DNA and concentrated elution performance of the Quick-DNA™ Miniprep Plus Kit are maintained across all 200 preparations — providing consistent, high-quality genomic DNA for long-read sequencing, NGS library preparation, and other demanding downstream applications at the throughput scale that the D4069 enables.
RNA-Free DNA — No RNase A Treatment Across 200 Preps Purified DNA is RNA-free, removing the need for RNase A treatments and ensuring accurate quantification for applications like library preparations. The RNA-free property is maintained consistently across all 200 preparations of the D4069 — critical for high-throughput NGS library preparation workflows where RNA contamination would affect library quality metrics and sequencing results at scale.
20-Minute Procedure — Maintained at High Volume Simple 20-minute procedure. The streamlined protocol completes in approximately 20 minutes per prep — maintained across all 200 preparations of the D4069. For high-volume labs and core facilities processing many samples per day, the consistent 20-minute prep time allows accurate throughput scheduling and capacity planning across the full kit lifetime.
Room Temperature Buffer Storage Most kit components stored at room temperature — BioFluid & Cell Buffer (Red), Solid Tissue Buffer (Blue), Genomic Binding Buffer, DNA Pre-Wash Buffer, g-DNA Wash Buffer, DNA Elution Buffer, Zymo-Spin™ IIC-XLR Columns, and Collection Tubes. Proteinase K & Storage Buffer stored at −20°C after reconstitution.
Complete Kit Contents — D4069 (200 Preps):
| Component | D4069 Quantity | Storage |
|---|---|---|
| Proteinase K & Storage Buffer | 4×20mg | −20°C after mixing |
| BioFluid & Cell Buffer (Red) | 45mL | Room temperature |
| Solid Tissue Buffer (Blue) | 22mL | Room temperature |
| Genomic Binding Buffer | 85mL | Room temperature |
| DNA Pre-Wash Buffer | 2×50mL | Room temperature |
| g-DNA Wash Buffer | 200mL | Room temperature |
| DNA Elution Buffer | 50mL | Room temperature |
| Zymo-Spin™ IIC-XLR Columns | 200 | Room temperature |
| Collection Tubes | 400 | Room temperature |
| Instruction Manual | 1 | — |
Key Features
- Zymo Research Quick-DNA™ Miniprep Plus Kit — Catalog # D4069
- 200 preparations with Zymo-Spin™ IIC-XLR Columns — high-volume format
- Universal sample compatibility — biological fluids, solid tissue, cells, FFPE, bacteria, blood cards
- Proteinase K included — 4×20mg for maintained activity across all 200 preparations
- Color-coded lysis buffers — BioFluid & Cell Buffer (Red), Solid Tissue Buffer (Blue)
- 25µg DNA binding capacity per column
- DNA yield from whole blood: 3–7µg per 100µL
- Tissue yields: 1–3µg per mg (skeletal, heart, lung, brain); 3–5µg per mg (liver, kidney)
- >50kb high molecular weight DNA
- Minimum elution volume: ≥35µL
- RNA-free — no RNase A treatment required
- No buffer retention — Zymo-Spin columns
- 20-minute procedure
- Recover 3× more DNA vs. standard kits
- Ready for qPCR, NGS, DNA-seq, arrays, methylation analysis
- Room temperature buffer storage (Proteinase K at −20°C after reconstitution)
- Lowest per-prep cost in the Quick-DNA™ Miniprep Plus line
- 1-year kit component integrity guarantee
- 50-prep version: D4068
Supported Applications High-throughput genomic DNA extraction from whole blood, buffy coat, solid tissue, FFPE samples, cultured mammalian cells, E. coli and bacteria, saliva, buccal cells, sputum, semen, blood storage cards (Guthrie, FTA®), avian blood, and viral DNA from serum and plasma — for use in PCR, qPCR, Sanger sequencing, next-generation sequencing (NGS) library preparation, long-read sequencing, restriction endonuclease digestion, Southern blotting, RFLP analysis, microsatellite genotyping, bisulfite conversion and DNA methylation analysis, CRISPR genome editing genotyping, pharmacogenomics, microarray analysis, and any high-throughput molecular biology workflow requiring high-yield, high molecular weight, RNA-free genomic DNA from the broadest possible range of sample types in 20 minutes per prep.
Technical Specifications
| Specification | Value |
|---|---|
| Catalog # | D4069 |
| Manufacturer | Zymo Research |
| Kit Name | Quick-DNA™ Miniprep Plus Kit |
| Preparations | 200 |
| Column Type | Zymo-Spin™ IIC-XLR |
| DNA Binding Capacity | 25µg per column |
| DNA Yield (Whole Blood) | 3–7µg per 100µL |
| DNA Yield (Skeletal/Heart/Lung/Brain) | 1–3µg per mg tissue |
| DNA Yield (Liver/Kidney) | 3–5µg per mg tissue |
| DNA Size | >50kb |
| Minimum Elution Volume | ≥35µL |
| Sample Types | Blood, tissue, cells, FFPE, bacteria, saliva, sputum, semen, blood cards, avian blood, viral |
| Proteinase K | Included — 4×20mg |
| Color-Coded Buffers | Yes — Red (BioFluid/Cell), Blue (Solid Tissue) |
| RNA-Free | Yes — selective binding, no RNase A required |
| Buffer Retention | Zero — Zymo-Spin columns |
| Preparation Time | ~20 minutes |
| Proteinase K Storage | −20°C after reconstitution |
| Buffer Storage | Room temperature (most components) |
| Kit Integrity Guarantee | 1 year from date of purchase |
| Smaller Pack Size | D4068 (50 preps) |
