Zymo Research DNA Clean & Concentrator™-5 D4004 | 200 Preps with Zymo-Spin™ I Columns (Uncapped)

The Zymo Research DNA Clean & Concentrator™-5 D4004 is the 200-preparation, high-volume PCR purification and DNA cleanup kit with uncapped Zymo-Spin™ I Columns — providing purification of up to 5µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, and other sources. Clean and concentrate up to 5µg DNA with ≥6µl elution in as little as 2 minutes with 0µl wash residue carryover. Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer. Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc. Supplied with uncapped Zymo-Spin™ I Columns. 200 preparations. Catalog # D4004. The 50-prep uncapped version is D4003. The capped column versions are D4013 (50 preps) and D4014 (200 preps).

D4004 vs. D4003 — Choosing the Right Kit Size: The D4004 and D4003 are identical DNA Clean & Concentrator™-5 kits in every way — the number of preparations and reagent volumes are the sole differences:

The D4004 is the preferred choice for high-throughput PCR labs, core genomics facilities, and any environment where DNA cleanup is a routine, high-frequency daily workflow step — reducing kit reordering frequency, providing the lowest per-prep reagent cost in the uncapped DCC-5 line, and minimizing workflow interruption from kit changeovers.

D4004 vs. D4014 — Uncapped vs. Capped at 200 Preps:

The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Some people prefer uncapped columns for faster sample processing while others prefer capped columns for easy labeling or added security.

Complete DCC-5 Family — All Catalog Numbers:

2-Minute DNA Cleanup — The Fastest Spin Column Kit at High Volume Clean and concentrate up to 5µg DNA with ≥6µl elution in as little as 2 minutes with 0µl wash residue carryover. The 2-minute protocol is maintained across all 200 preparations of the D4004 — allowing high-throughput labs and core facilities to process large batches of samples rapidly and consistently. For labs running 50, 100, or more DNA cleanup reactions per day, the 2-minute DCC-5 protocol delivers dramatically faster total batch processing time than 15-minute competing kits.

Up to 5µg DNA Per Prep — 70–95% Recovery from 50bp to 10kb ≤5µg total DNA can be recovered. For DNA 50bp to 10kb the recovery is 70–95%. For DNA 11kb to 23kb the recovery is 50–70%. Recovery is maintained consistently across all 200 preparations of the D4004 — ensuring reliable, reproducible cleanup performance throughout the full kit lifetime in high-throughput environments.

≥6µL Minimum Elution — Maximum DNA Concentration at High Throughput The ≥6µL minimum elution volume of the DCC-5 produces the most highly concentrated DNA eluate available from a standard PCR cleanup kit — maintained across all 200 preparations. For high-throughput sequencing facilities preparing hundreds of samples for NGS library preparation, the concentrated elution volume of the D4004 directly improves library preparation efficiency and downstream sequencing results.

Zero Buffer Retention — No Wash Residue Carryover Across 200 Preps Zymo-Spin™ columns are designed for complete elution with no buffer retention or carryover. The zero buffer retention property of the Zymo-Spin™ I column is maintained across all 200 preparations — ensuring clean eluates free from wash buffer inhibition in every prep throughout the kit lifetime. This is especially critical in high-throughput environments where downstream enzymatic reactions such as ligation, restriction digestion, and sequencing must perform consistently across large sample batches.

Broad Sample Compatibility The D4004 facilitates removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Compatible sample sources include:

• PCR reactions — standard, long-range, high-fidelity, hot-start
• Restriction endonuclease digests — single and double digest cleanup
• DNA modification reactions — end repair, A-tailing, ligation, dephosphorylation/phosphorylation
• Isotope and fluorescence labeling reactions — unincorporated label removal
• NGS library preparation — adapter ligation cleanup, end repair cleanup
• Bisulfite conversion — pre- and post-conversion cleanup
• Sequencing reactions — post-cycle sequencing dye terminator cleanup

High Purity DNA — A260/A280 ≥1.8 Across All 200 Preps High-quality DNA (A260/A280 ≥1.8) ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures — maintained consistently across all 200 preparations of the D4004.

Room Temperature Storage — All Kit Components All kit components stored at room temperature — simplifying storage of the larger D4004 reagent volumes in high-throughput lab environments. Note: Ethanol must be added to DNA Wash Buffer concentrate prior to use as indicated on the label.

Complete Kit Contents — D4004 (200 Preps):

Compatible Replacement — Cross-Reference Guide

The Zymo Research DNA Clean & Concentrator™-5 D4004 is a comparable alternative to high-volume PCR purification kits from several major manufacturers:

• Qiagen QIAquick PCR Purification Kit (28106) — 250-prep PCR cleanup kit, 30µL minimum elution
• Thermo Fisher GeneJET PCR Purification Kit (K0702) — 250-prep PCR cleanup kit
• NEB Monarch PCR & DNA Cleanup Kit (T1030L) — large-format PCR cleanup kit
• Macherey-Nagel NucleoSpin Gel and PCR Clean-up (740609.250) — 250-prep PCR cleanup kit

The D4004 differentiates with ≥6µL minimum elution (vs. 30µL for QIAquick), 2-minute protocol, and zero buffer retention across all 200 preparations. These products may differ in protocol, yield, and performance characteristics.

Key Features

• Zymo Research DNA Clean & Concentrator™-5 — Catalog # D4004
• 200 preparations with Zymo-Spin™ I Columns (Uncapped) — high-volume format
• Up to 5µg total DNA per prep
• ≥6µL minimum elution volume — maximum DNA concentration
• 2-minute cleanup procedure — fastest spin column DNA cleanup available
• Zero buffer retention — no wash residue carryover, no dry spin required
• DNA recovery: 70–95% for 50bp–10kb; 50–70% for 11kb–23kb
• DNA size range: ~50bp to 23kb
• A260/A280 ≥1.8 — high-purity DNA across all 200 preparations
• Removes DNA polymerases, restriction enzymes, ligases, kinases, nucleases, dNTPs, and labeled analogs
• Uncapped columns — faster processing (capped version: D4014)
• Room temperature storage — all kit components
• Ethanol must be added to DNA Wash Buffer concentrate before use
• 1-year kit component integrity guarantee
• Lot-to-lot tested for performance and reliability
• Lowest per-prep cost in the uncapped DCC-5 line
• 50-prep uncapped version: D4003
• Capped versions: D4013 (50 preps), D4014 (200 preps)

Supported Applications High-volume PCR product cleanup and desalting, large-batch restriction endonuclease digest purification, high-throughput NGS library preparation cleanup (adapter ligation, end repair, size selection), core facility DNA cleanup services, high-throughput Sanger sequencing template preparation, DNA modification reaction cleanup at scale, isotope and fluorescence label removal, bisulfite conversion cleanup, CRISPR donor DNA preparation at scale, transfection-grade PCR product preparation in large batches, and any high-throughput molecular biology workflow requiring rapid, highly concentrated, ultra-pure DNA in 2 minutes per prep.

Technical Specifications