Product Description
LIGHT LABS is proud to offer the Zymo Research Zyppy™-96 Plasmid MagBead Miniprep Kit (Cat # D4100) — the fastest and most innovative high-throughput 96-well plasmid DNA purification kit available, combining Zymo Research's proven Zyppy™ alkaline lysis chemistry with cutting-edge magnetic bead technology to deliver the highest-quality endotoxin-free plasmid DNA from 96 samples simultaneously — without a centrifuge. The Zyppy™-96 MagBead Miniprep completely eliminates the most time-consuming steps of conventional 96-well plasmid purification: cell pelleting, resuspension, and centrifugation — dramatically reducing hands-on time and enabling fully automated high-throughput plasmid DNA workflows.
The pellet-free procedure begins by adding Zymo Research's uniquely formulated Deep Blue Lysis Buffer directly to bacterial cultures in a 96-well block — bypassing the cell-pelleting and resuspension steps entirely. The patented Deep Blue Lysis Buffer technology provides instant visual confirmation of complete bacterial cell lysis and neutralization through a distinctive color change — eliminating the guesswork from one of the most critical steps of the plasmid miniprep procedure. Magnetic beads then capture the plasmid DNA from the clarified lysate, a magnetic stand pellets the beads for wash steps, and pure plasmid DNA is eluted — all without centrifugation. The resulting plasmid DNA is endotoxin-free and ready for PCR, sequencing, cloning, transfection, and other demanding downstream applications. Compatible with both automated liquid handling platforms and manual processing. Genesee Scientific catalog number is 11-310M. LIGHT LABS is your trusted source for Zymo Research products.
KEY FEATURES
- Magnetic bead technology — innovative MagBead purification eliminates centrifugation entirely; all wash and elution steps performed on a magnetic stand
- Pellet-Free procedure — Deep Blue Lysis Buffer added directly to bacterial cultures in 96-well block; no cell pelleting, no resuspension, no centrifuge required
- Fastest high-throughput 96-well plasmid purification available — dramatically reduces hands-on time vs. conventional spin-column 96-well miniprep methods
- Patented Deep Blue Lysis Buffer — distinctive color change provides instant visual confirmation of complete bacterial cell lysis and neutralization
- Patented colored buffer technology — eliminates guesswork from lysis verification for consistent, reproducible results across all 96 wells
- Endotoxin-free plasmid DNA — suitable for transfection, viral vector production, and other endotoxin-sensitive applications
- High-quality plasmid DNA ready for PCR, sequencing, cloning, transfection, and other downstream applications
- Compatible with both automated liquid handling systems and manual processing — ideal for core facilities and production environments
- 2 x 96 prep format — process up to 192 samples in two 96-well runs
- Genesee Scientific Catalog No. 11-310M
- Made in the USA by Zymo Research
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | D4100 |
| Genesee Scientific Catalog No. | 11-310M |
| Brand | Zymo Research |
| Kit Name | Zyppy™-96 Plasmid MagBead Miniprep Kit |
| Number of Preps | 2 x 96 (192 total) |
| Format | 96-Well, Magnetic Bead-Based |
| Lysis Method | Modified alkaline lysis — Deep Blue Lysis Buffer (pellet-free, direct lysis) |
| Lysis Verification | Patented color change (Deep Blue Lysis Buffer) |
| Purification Method | Magnetic bead binding — no centrifuge required |
| Centrifuge Required | No |
| Cell Pelleting Required | No (pellet-free) |
| Endotoxin Level | Endotoxin-free |
| Processing Method | Manual (magnetic stand) or automated liquid handling |
| Downstream Applications | PCR, sequencing, cloning, transfection, viral vector production |
| Storage Temperature | Room temperature |
APPLICATIONS
- High-throughput 96-well plasmid DNA miniprep from E. coli
- Automated plasmid DNA purification for synthetic biology and genome engineering workflows
- Core facility high-throughput plasmid preparation
- Plasmid DNA preparation for transfection requiring endotoxin-free quality
- CRISPR construct plasmid DNA preparation at high throughput
- Plasmid library preparation and screening
- Colony picking and plasmid verification at 96-well scale
- Any high-throughput plasmid miniprep application requiring no centrifugation and maximum speed
ORDERING INFORMATION — ZYMO RESEARCH ZYPPY™-96 PLASMID MAGBEAD MINIPREP KIT
| Cat # | Description | Preps |
|---|---|---|
| D4100 | Zyppy™-96 Plasmid MagBead Miniprep Kit | 2 x 96 |
| D4101 | Zyppy™-96 Plasmid MagBead Miniprep Kit | 4 x 96 |
| D4102 | Zyppy™-96 Plasmid MagBead Miniprep Kit | 8 x 96 |
| D4041 | Zyppy™-96 Plasmid Miniprep Kit (spin column) | 2 x 96 |
| D4043 | Zyppy™-96 Plasmid Miniprep Kit (spin column) | 8 x 96 |
| D4201 | ZymoPURE™ II Plasmid Midiprep Kit | 50 preps |
| D4203 | ZymoPURE™ II Plasmid Maxiprep Kit | 20 preps |
| D4027 | Zyppy™ Plasmid Maxiprep Kit | 10 preps |
