Product Description
LIGHT LABS is proud to offer the Zymo Research Zyppy™-96 Plasmid Miniprep Kit (Cat # D4043) — the highest-volume 8 x 96 prep format of the fastest and simplest high-throughput plasmid DNA purification kit available. Purpose-built for core facilities, contract research organizations (CROs), high-throughput screening labs, and any research group running large numbers of 96-well plasmid preparations on a routine basis, the D4043 delivers the same outstanding pellet-free performance as the smaller D4041 and D4042 formats with maximum economy and capacity.
The Zyppy™-96 kit features an innovative pellet-free, direct lysis procedure that completely bypasses the conventional bacterial culture centrifugation, pelleting, and resuspension steps. The uniquely formulated Deep Blue Lysis Buffer is added directly to bacterial cultures growing in the supplied 96-well block — no pre-centrifugation of cultures required. The patented yellow Neutralization/Clearing Buffer then assists with lysate neutralization and clearing. Combined with Zymo-Spin™ I-96 plate technology, the result is endotoxin-free, high-quality plasmid DNA (A260/280 ≥ 1.8, up to 10 µg/well) in approximately 45 minutes — immediately ready for restriction endonuclease digestion, ligation, PCR, Sanger sequencing, NGS library preparation, transfection, and other sensitive downstream applications. LIGHT LABS is your trusted source for Zymo Research products.
KEY FEATURES
- Fastest and simplest high-throughput 96-well plasmid miniprep workflow available
- 8 x 96 prep maximum-volume format — ideal for core facilities, CROs, and high-throughput screening labs
- Pellet-free direct lysis procedure — Deep Blue Lysis Buffer added directly to bacterial cultures in the 96-well block; no pre-centrifugation, no pelleting, no resuspension
- Patented color-coded buffer system (Deep Blue Lysis Buffer + yellow Neutralization/Clearing Buffer) for easy visual confirmation of complete bacterial lysis and neutralization
- Endotoxin-free plasmid DNA — suitable for transfection, viral vector production, and other sensitive applications
- High DNA purity — typical A260/280 ≥ 1.8
- Up to 10 µg plasmid DNA yield per well (depending on plasmid copy number, culture conditions, and E. coli strain)
- Plasmid size range: up to 25 kb
- Minimum elution volume: ≥ 30 µl
- Processing time: approximately 45 minutes for 96 samples
- Requires centrifuge with microplate carriers
- Eluted DNA immediately ready for restriction digestion, ligation, PCR, sequencing, transfection, and in vitro transcription
- Genesee Scientific Catalog No. 11-311
- Made in the USA by Zymo Research
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | D4043 |
| Genesee Scientific Catalog No. | 11-311 |
| Brand | Zymo Research |
| Kit Name | Zyppy™-96 Plasmid Miniprep Kit |
| Format | 96-Well Plate (Zymo-Spin™ I-96) |
| Number of Preps | 8 x 96 (768 total) |
| DNA Yield | Up to 10 µg per well |
| DNA Purity | A260/280 ≥ 1.8 |
| Plasmid Size Range | Up to 25 kb |
| Minimum Elution Volume | ≥ 30 µl |
| Processing Time | ~45 minutes |
| Lysis Method | Pellet-free direct lysis (Deep Blue Lysis Buffer added directly to culture) |
| Buffer System | Patented color-coded: Deep Blue Lysis Buffer, Yellow Neutralization/Clearing Buffer |
| Equipment Required | Centrifuge with microplate carriers |
| Endotoxin-Free | Yes |
| Storage Temperature | Room temp (Neutralization/Clearing Buffer: 4–8°C) |
| Kit Components | Deep Blue Lysis Buffer (4 x 30 mL), Neutralization/Clearing Buffer/Yellow (4 x 100 mL), Endo-Wash Buffer (4 x 60 mL), Zyppy™ Wash Buffer concentrate (4 x 24 mL), Zyppy™ Elution Buffer (4 x 30 mL), 96-Well Blocks (8), Collection Plates (8), Zymo-Spin™ I-96 Plates (8), Elution Plates (8), Air-Permeable Sealing Covers (8) |
APPLICATIONS
- High-throughput plasmid DNA isolation from E. coli at maximum scale
- Core facility and CRO high-throughput plasmid DNA production
- Colony screening and clone verification by restriction digest or PCR
- Sanger DNA sequencing
- NGS library preparation and plasmid sequencing
- DNA ligation and cloning workflows
- PCR template preparation
- Transfection-grade plasmid DNA preparation
- Viral vector (lentiviral, AAV, adenoviral) plasmid production
- In vitro transcription
- High-throughput drug discovery and screening workflows requiring plasmid DNA
ORDERING INFORMATION
| Cat # | Description | Format | Yield/Well | Preps |
|---|---|---|---|---|
| D4043 | Zyppy™-96 Plasmid Miniprep Kit | 96-Well Spin Plate | Up to 10 µg | 8 x 96 |
| D4042 | Zyppy™-96 Plasmid Miniprep Kit | 96-Well Spin Plate | Up to 10 µg | 4 x 96 |
| D4041 | Zyppy™-96 Plasmid Miniprep Kit | 96-Well Spin Plate | Up to 10 µg | 2 x 96 |
| D4102 | Zyppy™-96 Plasmid MagBead Miniprep Kit | 96-Well Magnetic Bead | Up to 10 µg | 8 x 96 |
| D4101 | Zyppy™-96 Plasmid MagBead Miniprep Kit | 96-Well Magnetic Bead | Up to 10 µg | 4 x 96 |
| D4100 | Zyppy™-96 Plasmid MagBead Miniprep Kit | 96-Well Magnetic Bead | Up to 10 µg | 2 x 96 |
| D4020 | Zyppy™ Plasmid Miniprep Kit (spin column) | Spin Column | Up to 25 µg | 400 |
| D4037 | Zyppy™ Plasmid Miniprep Kit (spin column) | Spin Column | Up to 25 µg | 800 |
