Product Description
LIGHT LABS is proud to offer the Zymo Research Zymoclean™ Gel RNA Recovery Kit (Cat # R1011) — a fast, highly efficient spin column kit for the purification and recovery of high-quality RNA fragments from agarose gels. Combining a unique single-step RAD (RNA Agarose Dissolving) Buffer that simultaneously dissolves the agarose gel slice and binds the RNA with Zymo-Spin™ IC capped column technology, the R1011 delivers ultra-pure, concentrated RNA in approximately 30 minutes without the need for phenol/chloroform, organic solvents, or messy precipitation steps.
The Zymoclean™ Gel RNA Recovery Kit recovers RNA fragments ≥200 nucleotides from agarose gels buffered with TAE, TBE, or MOPS — including formaldehyde denaturing gels up to 2.0% — making it compatible with the full range of RNA gel electrophoresis formats commonly used in research labs. Recovery rates of ≥80% are achieved for RNA fragments ≥500 nt. The patented Fast-Spin Zymo-Spin™ IC column technology allows RNA to be concentrated and eluted into minimal volumes (≥ 6 µl) of DNase/RNase-free water, yielding highly concentrated, ultra-pure RNA ready for all downstream RNA-based manipulations. LIGHT LABS is your trusted source for Zymo Research products.
KEY FEATURES
- Fast 30-minute RNA recovery from agarose gels — no phenol, chloroform, or precipitation required
- Unique single-step RAD Buffer simultaneously dissolves agarose and binds RNA — simplifying the workflow vs. competing kits
- ≥80% RNA recovery for fragments ≥500 nt
- Compatible with RNA fragments ≥200 nt resolved in TAE, TBE, and MOPS buffered agarose gels
- Compatible with formaldehyde denaturing agarose gels (up to 2.0% formaldehyde)
- Compatible with both single-stranded (ssRNA) and double-stranded RNA (dsRNA)
- Up to 10 µg RNA binding capacity per column
- Fast-Spin Zymo-Spin™ IC Capped columns — concentrated elution into minimal volumes (≥ 6 µl) for highly concentrated samples
- Eluted RNA is ultra-pure and ready for RT-PCR, Northern blotting, in vitro translation, RNA sequencing, microinjection, and other downstream applications
- Maximum gel slice input: up to 400 mg agarose per column
- Fisher Scientific Catalog No. NC1027310
- Genesee Scientific Catalog No. 11-351
- Made in the USA by Zymo Research
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | R1011 |
| Fisher Scientific Catalog No. | NC1027310 |
| Genesee Scientific Catalog No. | 11-351 |
| Brand | Zymo Research |
| Kit Name | Zymoclean™ Gel RNA Recovery Kit |
| Column Type | Zymo-Spin™ IC (Capped) |
| Number of Preps | 50 |
| RNA Binding Capacity | Up to 10 µg per column |
| RNA Fragment Size | ≥200 nt (≥80% recovery for fragments ≥500 nt) |
| RNA Type | Single-stranded (ssRNA) and double-stranded RNA (dsRNA) |
| Gel Compatibility | TAE, TBE, and MOPS buffered agarose gels; formaldehyde up to 2.0% |
| Maximum Gel Input | Up to 400 mg agarose per column |
| Minimum Elution Volume | ≥ 6 µl |
| Processing Time | ~30 minutes |
| Equipment Required | Microcentrifuge; 37–65°C heat source |
| Storage Temperature | Room temperature |
| Kit Components | RAD Buffer (RNA Agarose Dissolving Buffer) (10 mL), RNA Wash Buffer concentrate (6 mL), DNase/RNase-free Water (1 mL), Zymo-Spin™ IC Capped Columns (50), Collection Tubes (50), Instruction Manual |
APPLICATIONS
- RNA fragment isolation and purification from preparative agarose gels
- mRNA and rRNA gel band recovery
- RNA size selection via gel electrophoresis and recovery
- RNA fragment purification for in vitro translation
- RT-PCR and qRT-PCR template preparation from gel-purified RNA
- Northern blot probe preparation and RNA isolation
- RNA sequencing and transcriptomics sample preparation
- Microinjection-grade RNA preparation
- Any application requiring the recovery of specific RNA fragments from agarose gels
ORDERING INFORMATION
| Cat # | Description | Column Type | Capacity | Preps |
|---|---|---|---|---|
| R1011 | Zymoclean™ Gel RNA Recovery Kit | Capped (IC) | 10 µg | 50 |
| R1011-1-50 | RAD Buffer (RNA Agarose Dissolving Buffer) only | — | — | 50 mL |
| R1015 | RNA Clean & Concentrator™-5 | Capped (IC) | 5 µg | 50 |
| R1016 | RNA Clean & Concentrator™-5 | Capped (IC) | 5 µg | 200 |
| R1013 | RNA Clean & Concentrator™-5 with DNase I Set | Capped (IC) | 5 µg | 50 |
| R1014 | RNA Clean & Concentrator™-5 with DNase I Set | Capped (IC) | 5 µg | 200 |
| R1017 | RNA Clean & Concentrator™-25 | Capped (IIC) | 25 µg | 50 |
| R1018 | RNA Clean & Concentrator™-25 | Capped (IIC) | 25 µg | 100 |
