Product Description
Get substantially more plasmid from your yeast cultures while skipping the precipitation step. This Zymo Research miniprep recovers roughly five times more plasmid DNA than the Zymoprep I format and uses spin-column purification in place of isopropanol precipitation, saving time while improving yield from colonies, plate patches or liquid cultures.
This listing is for catalog number D2004: Zymoprep Yeast Plasmid Miniprep II, 50 Preps. The workflow combines Zymolyase digestion with alkaline lysis and neutralization, then binds plasmid DNA to a Zymo-Spin column for washing and elution. No glass beads or phenol are needed. The kit supports S. cerevisiae, C. albicans, S. pombe and other susceptible fungi, and the eluted DNA is ready for E. coli transformation, PCR or Southern blotting. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Higher recovery – Yields about five times more plasmid DNA than Zymoprep I.
- No precipitation step – Column purification eliminates isopropanol precipitation.
- Bead- and phenol-free – Enzymatic digestion replaces mechanical disruption and organic extraction.
- Ideal for difficult plasmids – Suited to low-copy and hard-to-isolate constructs.
- Flexible inputs – Works from colonies, patches or liquid cultures.
Specifications
| Catalog Number | D2004 |
| Brand | Zymo Research |
| Description | Zymoprep Yeast Plasmid Miniprep II, 50 Preps |
| Format | 50 preps |
| Relative yield | About 5-fold more plasmid DNA than Zymoprep I |
| Compatible organisms | S. cerevisiae, C. albicans, S. pombe and fungi susceptible to yeast lytic enzyme |
| Sample types | Colonies, plate patches, liquid cultures |
| Purification | Zymo-Spin column; no isopropanol precipitation |
| Shipping Conditions | Ships at room temperature |
| Storage | -20°C |
| Note | Product contains multiple components shipped separately. [Includes E1004 x 1: Zymolyase (1000 Units Lyophilized) w/ Storage Buffer (500µl)] |
Applications
- Yeast two-hybrid plasmid rescue
- Shuttle vector recovery for E. coli transformation
- PCR analysis of yeast plasmids
- Southern blot analysis
