CELLTREAT® 229716 | 250mL Bottle Top Filter, 0.22µm PES, 75mm Diameter, Sterile — No Bottle Included

The CELLTREAT® 229716 is a 250mL bottle top filter with upgraded 0.22µm PES membrane — sterile, with upgraded membranes that filter up to 43% faster, a 75mm diameter membrane, and PES membrane designed for high flow rate with low binding. 0.22µm PES membrane is the first choice for sterile filtration of cell culture media. The 229716 is the standalone bottle top filter format — the sterile filter component only, designed for labs that already have CELLTREAT solution bottles or other standard 45mm threaded bottles and need only the filter. Bottle top filters fit on CELLTREAT® solution bottles or other standard 45mm threaded solution storage bottles. Bottle top filters do not include solution bottles. Sterile by gamma irradiation. RNase-free, DNase-free, and non-pyrogenic.

⚠️ Critical Distinction — Bottle Top Filter Only. Solution Bottle NOT Included. The 229716 is the filter component only — it does not include a solution bottle or cap. This is the key difference from the CELLTREAT 229706 which is a complete Filter System including bottle top filter, solution bottle, and cap. The 229716 is the correct choice for labs that:

• Already have CELLTREAT solution bottles or compatible 45mm threaded bottles
• Want to purchase filter tops and bottles separately for cost or inventory flexibility
• Are replacing only the filter component after reusing an existing bottle
• Prefer to use a specific bottle brand or size not included in the complete filter system packages

For a complete ready-to-use filtration system including solution bottle and cap, see the CELLTREAT 229706 (250mL, 0.22µm PES Filter System).

229716 vs. 229706 — Bottle Top Filter Only vs. Complete Filter System:

Upgraded 0.22µm PES Membrane — 43% Faster Sterile Filtration New! Upgraded 0.22µm PES filter membranes now filter up to 43% faster. The upgraded membrane formulation of the 229716 delivers meaningfully faster sterile filtration than previous-generation 0.22µm membranes — reducing the time required to filter a full 250mL volume of cell culture media, serum-containing solutions, or biological buffers while maintaining the complete bacterial and mycoplasma retention required for sterile filtration assurance.

0.22µm PES — Sterile Filtration Standard for Cell Culture Media 0.22µm PES membrane is the first choice for sterile filtration of cell culture media. The 0.22µm pore size removes bacteria, mycoplasma, and other microorganisms ≥0.22µm while passing proteins, growth factors, vitamins, and other critical media components. The hydrophilic PES membrane provides high flow rate with low protein binding — minimizing loss of serum proteins, cytokines, enzymes, and growth factors during sterile filtration of supplemented media.

75mm Membrane Diameter — Large Diameter Reduces Clogging Improved filter support provides faster flow rates. The 75mm membrane diameter of the 229716 provides significantly more filtration surface area than the 50mm membranes of the 150mL bottle top filters — distributing the 250mL particulate load across a wider area, reducing local fouling and clogging, and maintaining higher flow rates throughout the complete filtration volume.

Easy-to-Read Molded Graduations and Bold Pore Size Identification Easy-to-read molded graduations on bottle top filter. Housings feature boldly printed membrane material and pore size for easy identification. The 0.22µm pore size is boldly printed on the filter housing — critical in labs where both 229716 (0.22µm) and 229702-equivalent 0.45µm bottle top filters are stocked together.

Fits Standard 45mm Threaded Bottles — Broad Compatibility Bottle top filters fit on CELLTREAT® solution bottles or other standard 45mm threaded solution storage bottles. The 229716 is compatible with the full CELLTREAT solution bottle family as well as other standard 45mm threaded bottles — providing maximum flexibility for labs with existing bottle inventories from any major laboratory supplier.

Angled Hose Barb Adapter, Curved Design, and Improved Filter Support Angled hose barb adapter fits three size hose diameters and prevents the filter from tipping. Curved bottle design for easy handling. The angled barb accommodates 6mm, 9mm, and 12mm ID vacuum tubing without adapters, prevents tipping during filtration, and the improved support grid sustains high flow rates throughout the complete 250mL filtration volume.

Lot Number and Expiration Date on Every Bottle Top Printed lot number and expiration date on every bottle top. Full lot traceability for regulated cell culture environments and GMP documentation requirements.

Compatible Replacement — Cross-Reference Guide

The CELLTREAT® 229716 is a direct compatible replacement for 250mL 0.22µm PES standalone bottle top filters from several major manufacturers:

• Millipore® SCGVUSS02RE — Stericup 250mL 0.22µm PES bottle top filter only
• Thermo Fisher Nalgene™ 296-4520 — Nalgene 250mL 0.22µm PES bottle top filter
• VWR® 10040-458 — VWR 250mL 0.22µm PES bottle top filter, no bottle

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific bottles and protocol.

Complete CELLTREAT® Bottle Top Filter Family — Key Formats:

Key Features

• 250mL bottle top filter — filter component only, no solution bottle included
• Upgraded 0.22µm PES membrane — filters up to 43% faster than previous generation
• Sterile filtration — removes bacteria and mycoplasma ≥0.22µm
• Fits CELLTREAT solution bottles and all standard 45mm threaded solution storage bottles
• 75mm membrane diameter — large diameter reduces clogging vs. 50mm membranes
• Hydrophilic PES membrane — high flow rate with low protein binding
• Improved filter support — sustained flow rates under vacuum
• Easy-to-read molded graduations on bottle top filter
• Bold printed membrane material and 0.22µm pore size — easy identification
• Angled hose barb adapter — fits 6mm, 9mm, and 12mm ID tubing, prevents tipping
• Lot number and expiration date printed on every bottle top
• Sterile — gamma irradiation
• RNase-free and DNase-free — certified
• Non-pyrogenic

Supported Applications Sterile filtration of 250mL volumes of cell culture media, preparation of supplemented growth media in mid-size batches, sterile buffer preparation, sterile filtration of specialized cell culture reagents and supplements, enzyme and protein solution sterilization, and any 250mL 0.22µm sterile filtration application where a compatible solution bottle is already available.

Technical Specifications