CELLTREAT® 229717 | 500mL Bottle Top Filter, 0.22µm PES, 90mm Diameter, Sterile — No Bottle Included

The CELLTREAT® 229717 is a 500mL bottle top filter with upgraded 0.22µm PES membrane — sterile, with upgraded membranes that filter up to 43% faster, a 90mm diameter membrane for high flow rate with low binding, and 0.22µm PES membrane that is the first choice for sterile filtration of cell culture media. Bottle top filters fit on CELLTREAT® solution bottles or other standard 45mm threaded solution storage bottles. Bottle top filters do not include solution bottles. The 229717 is the standalone bottle top filter format — the sterile filter component only, designed for labs that already have CELLTREAT solution bottles or other standard 45mm threaded bottles and need only the filter. Sterile by gamma irradiation. RNase-free, DNase-free, and non-pyrogenic.

⚠️ Critical Distinction — Bottle Top Filter Only. Solution Bottle NOT Included. The 229717 is the filter component only — it does not include a solution bottle or cap. This is the key difference from the CELLTREAT 229707 which is the complete 500mL 0.22µm Filter System including bottle top filter, solution bottle, and cap. The 229717 is the correct choice for labs that:

• Already have CELLTREAT solution bottles or compatible 45mm threaded bottles
• Want to purchase filter tops and bottles separately for cost or inventory flexibility
• Are replacing only the filter component after reusing an existing bottle
• Prefer to use a specific bottle brand or size not included in the complete system

For a complete ready-to-use filtration system including solution bottle and cap, see the CELLTREAT 229707 (500mL, 0.22µm PES Filter System).

229717 vs. 229707 — Bottle Top Filter Only vs. Complete Filter System:

229717 vs. 229713 — Choosing the Right Pore Size for 500mL Bottle Top Filters:

Upgraded 0.22µm PES Membrane — 43% Faster Sterile Filtration New! Upgraded 0.22µm PES filter membranes now filter up to 43% faster. The upgraded membrane formulation delivers meaningfully faster sterile filtration than previous-generation 0.22µm membranes — reducing the time required to filter a full 500mL volume of cell culture media or biological solutions while maintaining complete bacterial and mycoplasma retention.

0.22µm PES — Sterile Filtration Standard for Cell Culture Media 0.22µm PES membrane is the first choice for sterile filtration of cell culture media. The 0.22µm pore size removes bacteria, mycoplasma, and other microorganisms ≥0.22µm while passing proteins, growth factors, vitamins, and other critical media components. The hydrophilic PES membrane provides high flow rate with low protein binding — minimizing loss of serum proteins, cytokines, enzymes, and growth factors during sterile filtration.

90mm Membrane Diameter — Maximum Flow Area for 500mL Scale The 90mm membrane diameter provides the maximum filtration surface area in the CELLTREAT 500mL bottle top filter lineup — distributing the 500mL particulate load across a wide membrane area to reduce local fouling and clogging. For challenging samples such as serum-containing media or high-protein solutions, the 90mm membrane maintains high flow rates throughout the full filtration volume.

Easy-to-Read Molded Graduations and Bold Pore Size Identification Easy-to-read molded graduations on bottle top filter. Housings feature boldly printed membrane material and pore size for easy identification. The 0.22µm pore size is boldly printed on the filter housing — critical in labs where both 229717 (0.22µm sterile) and 229713 (0.45µm clarification) 500mL bottle top filters are stocked together.

Fits Standard 45mm Threaded Bottles — Broad Compatibility Bottle top filters fit on CELLTREAT® solution bottles or other standard 45mm threaded solution storage bottles. The 229717 is compatible with the full CELLTREAT solution bottle family as well as other standard 45mm threaded bottles from major laboratory suppliers — providing maximum flexibility for labs with existing bottle inventories.

Angled Hose Barb Adapter, Curved Design, and Improved Filter Support Angled hose barb adapter fits three size hose diameters and prevents the filter from tipping. Improved filter support provides faster flow rates. The angled barb accommodates 6mm, 9mm, and 12mm ID vacuum tubing without adapters, prevents tipping, and the improved support grid sustains high flow rates throughout the complete 500mL filtration volume.

Lot Number and Expiration Date on Every Bottle Top Printed lot number and expiration date on every bottle top. Full lot traceability for regulated cell culture environments and GMP documentation requirements.

Compatible Replacement — Cross-Reference Guide

The CELLTREAT® 229717 is a direct compatible replacement for 500mL 0.22µm PES standalone bottle top filters from several major manufacturers:

• Corning® 431118 — Corning 500mL bottle top filter, 0.22µm PES, no bottle
• Millipore® SCGVUSS05RE — Stericup 500mL 0.22µm PES bottle top filter only
• Thermo Fisher Nalgene™ 296-4520 — Nalgene 500mL 0.22µm PES bottle top filter
• VWR® 10040-460 — VWR 500mL 0.22µm PES bottle top filter, no bottle

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific bottles and protocol.

Complete CELLTREAT® Bottle Top Filter Family — Key Formats:

Key Features

• 500mL bottle top filter — filter component only, no solution bottle included
• Upgraded 0.22µm PES membrane — filters up to 43% faster than previous generation
• Sterile filtration — removes bacteria and mycoplasma ≥0.22µm
• Fits CELLTREAT solution bottles and all standard 45mm threaded solution storage bottles
• 90mm membrane diameter — maximum flow area for 500mL scale
• Hydrophilic PES membrane — high flow rate with low protein binding
• Improved filter support — sustained flow rates under vacuum
• Easy-to-read molded graduations on bottle top filter
• Bold printed membrane material and 0.22µm pore size — easy identification
• Angled hose barb adapter — fits 6mm, 9mm, and 12mm ID tubing, prevents tipping
• Lot number and expiration date printed on every bottle top
• Sterile — gamma irradiation
• RNase-free and DNase-free — certified
• Non-pyrogenic

Supported Applications Sterile filtration of 500mL volumes of cell culture media, large-batch supplemented media preparation using existing bottles, sterile buffer preparation, sterile filtration of protein and enzyme solutions, and any 500mL 0.22µm sterile filtration application where a compatible solution bottle is already available.

Technical Specifications