Product Description
LIGHT LABS is proud to offer the CELLTREAT 30mm x 10mm Glass Bottom Tissue Culture Treated Dish (Cat # 229632) — a premium, sterile cell culture dish specifically engineered for high-magnification live cell microscopy, confocal imaging, fluorescence microscopy, and polarized light microscopy applications requiring optical-quality glass as the culture substrate. The 229632 combines a polystyrene dish body with a recessed 15mm diameter borosilicate glass bottom of #1.5 coverslip thickness — the optical thickness standard for high-numerical-aperture oil-immersion objectives — delivering outstanding optical clarity and resolution for advanced imaging workflows.
The recessed glass bottom creates a convenient micro-well within the dish, concentrating cells and media in the imaging region while providing a flat, optically consistent viewing surface. Unlike plastic-bottom dishes that introduce aberrations at high magnification, the borosilicate glass bottom of the 229632 is optically clear and provides the optical path consistency required for advanced imaging modalities including confocal laser scanning microscopy, TIRF (total internal reflection fluorescence), spinning disk confocal, and super-resolution microscopy. The tissue culture treated polystyrene and glass surfaces promote consistent adherent cell attachment across the culture area.
Non-cytotoxic medical-grade adhesive bonds the glass to the polystyrene body — ensuring the adhesive does not inhibit cell growth or introduce autofluorescence artifacts during imaging. The dish features an innovative offset bottom design that makes the bottom of the dish protrude slightly — allowing users to easily pick up the dish from the bottom rather than accidentally grasping the lid, a critical ergonomic advantage when handling many dishes simultaneously. The vented lid features molded spacers for gas exchange and a stacking ring for incubator stability. Packaged 10 dishes per resealable bag, 5 bags per case (50 dishes total). LIGHT LABS is your trusted source for CELLTREAT laboratory plasticware.
KEY FEATURES
- #1.5 borosilicate glass bottom (0.17mm thickness) — the optical standard for high-numerical-aperture oil-immersion microscopy objectives; essential for confocal, TIRF, spinning disk, and super-resolution imaging
- Recessed 15mm diameter glass bottom creates a convenient micro-well for cell culture and imaging within a defined optical region
- Tissue culture treated (TC-treated) surface on both glass and polystyrene — promotes consistent adherent cell attachment for live cell imaging applications
- Non-cytotoxic medical-grade adhesive bonds glass to polystyrene body — does not inhibit cell growth or introduce autofluorescence imaging artifacts
- Unique offset bottom design — bottom of dish protrudes for easy one-hand pickup; prevents accidental grasping of the lid
- Vented lid with molded spacers — allows consistent gas exchange with CO₂ incubator atmosphere during cell culture
- Stacking ring on lid — increased stability when stacking dishes in the incubator
- Working volume: 1.4–3.5 mL; cell growth area: 1.77 cm²
- Polystyrene dish body with glass bottom — combines polystyrene ease-of-use with glass optical quality
- Sterile — gamma irradiated
- Certified nonpyrogenic and RNase-/DNase-free
- Packaged 10 dishes per resealable bag; 5 bags per case (50 dishes/case)
- Fisher Scientific Catalog No. 50-114-9057
- Compatible with inverted microscopes — ideal for live cell imaging on standard inverted microscope stages
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | 229632 |
| Fisher Scientific Catalog No. | 50-114-9057 |
| Brand | CELLTREAT Scientific Products |
| Product Name | 30mm x 10mm Glass Bottom TC-Treated Dish |
| Dish Diameter | 30 mm |
| Dish Height | 10 mm |
| Glass Bottom Diameter | 15 mm |
| Glass Bottom Thickness | #1.5 (0.17 mm) borosilicate coverslip thickness |
| Glass Type | Borosilicate (optically clear) |
| Adhesive | Non-cytotoxic medical-grade adhesive |
| Working Volume | 1.4–3.5 mL |
| Cell Growth Area | 1.77 cm² |
| Surface Treatment | Tissue Culture Treated (TC-Treated) — glass and polystyrene |
| Bottom Design | Recessed micro-well; offset bottom for easy handling |
| Lid Type | Vented (molded spacers for gas exchange) |
| Lid Additional Feature | Stacking ring for stability |
| Primary Material | Polystyrene (dish body) |
| Secondary Material | Borosilicate glass (bottom) |
| Color | Clear |
| Sterility | Sterile (gamma irradiated) |
| Certifications | Nonpyrogenic, RNase-free, DNase-free |
| Packaging | 10 dishes/resealable bag; 5 bags/case |
| Quantity | 50 dishes/case |
APPLICATIONS
- Live cell microscopy and time-lapse imaging on inverted microscopes
- Confocal laser scanning microscopy (CLSM)
- TIRF (total internal reflection fluorescence) microscopy
- Spinning disk confocal microscopy
- Super-resolution microscopy (STORM, PALM, STED)
- Fluorescence microscopy of adherent cells
- Polarized light microscopy
- Immunofluorescence staining and imaging of fixed cells
- High-magnification oil-immersion objective imaging
- Single-molecule imaging and tracking
- Cell morphology and dynamics studies requiring high optical resolution
ORDERING INFORMATION — CELLTREAT GLASS BOTTOM CELL CULTURE DISHES
| Cat # | Description | Dish Size | Glass Bottom | Glass Thickness | Working Volume | Qty/Case |
|---|---|---|---|---|---|---|
| 229632 | 30mm x 10mm Glass Bottom TC-Treated Dish, Sterile | 30mm x 10mm | 15mm diameter | #1.5 borosilicate | 1.4–3.5 mL | 50 |
| 229634 | 35mm x 10mm Glass Bottom TC-Treated Dish, Sterile | 35mm x 10mm | 20mm diameter | #1.5 borosilicate | 2.0–4.0 mL | 50 |
| 229636 | 60mm x 15mm Glass Bottom TC-Treated Dish, Sterile | 60mm x 15mm | 30mm diameter | #1.5 borosilicate | 3.0–5.0 mL | 50 |
| 229638 | 35mm x 10mm Non-Treated Petri Dish, Sterile | 35mm x 10mm | No glass bottom | — | 5 mL | 960 |
| 229690 | 100mm x 15mm TC-Treated Dish w/Grip Ring, Sterile | 100mm x 15mm | No glass bottom | — | 15–16 mL | 500 |
