Product Description
Need methylated DNA on demand? This modified nucleotide from Zymo Research lets you incorporate 5-methylcytosine directly during amplification, giving you full control over how much of the cytosine pool is methylated. That flexibility is valuable for building positive controls, partially methylated standards and test templates for epigenetic workflows.
This listing is for catalog number D1035: 5-Methyl dCTP (10 mM), 100µl. Supplied as a 10 mM stock solution, 5-Methyl dCTP can replace some or all of the standard dCTP in a nucleotide mixture. It is compatible with ZymoTaq, QuestTaq and other DNA polymerases used for routine PCR. Combine it with dATP, dGTP and dTTP to the ratio your experiment requires. The product ships on dry ice and should be kept frozen until use. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Adjustable methylation – Mix with standard dCTP at any ratio to tune methylation levels.
- Ready stock – 10 mM concentration simplifies dilution into custom nucleotide blends.
- Polymerase compatible – Suitable for ZymoTaq, QuestTaq and other DNA polymerases.
- Epigenetic controls – Creates methylated amplicons for assay calibration and validation.
- Shipped frozen – Dry ice delivery preserves nucleotide quality.
Specifications
| Catalog Number | D1035 |
| Brand | Zymo Research |
| Description | 5-Methyl dCTP (10 mM), 100µl |
| Concentration | 10 mM |
| Volume | 100 µl |
| Compatible polymerases | ZymoTaq, QuestTaq and other DNA polymerases |
| Shipping | Dry ice |
| Shipping Conditions | Ships on dry ice |
| Storage | -20°C |
Applications
- Methylated control DNA synthesis
- Partially methylated standard preparation
- Bisulfite sequencing validation
- Methylation-specific PCR development
