Product Description
Confirming bisulfite conversion efficiency and pipeline accuracy inside every library removes a major source of uncertainty in methylation sequencing. This spike-in control travels with your sample through library preparation and sequencing, providing an internal, unbiased readout of how well both the conversion and the analysis performed.
This listing is for catalog number D5500: Zymo-Seq Methyl Spike-in Control. The control consists of six double-stranded synthetic amplicons, 180 to 200 bp long, with distinct sequences derived from the E. coli K12 genome. Each carries a different CpG methylation level between 0% and 100%. Comparing observed with expected methylation for each amplicon reveals conversion efficiency and verifies that the bioinformatics pipeline is properly calibrated. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- In situ control – Spiked directly into NGS library preparations.
- Six methylation levels – Amplicons span 0% to 100% CpG methylation.
- Distinct sequences – Derived from the E. coli K12 genome for easy identification.
- Conversion readout – Provides an unbiased measure of bisulfite conversion efficiency.
- Pipeline calibration – Confirms correlation between observed and expected methylation.
Specifications
| Catalog Number | D5500 |
| Brand | Zymo Research |
| Description | Zymo-Seq Methyl Spike-in Control |
| Composition | Six synthetic dsDNA amplicons |
| Length | 180–200 bp |
| Sequence origin | E. coli K12 genome |
| Methylation levels | 0% to 100% CpG methylation |
| Shipping Conditions | Ships on dry ice |
| Storage | -20°C |
Applications
- WGBS and RRBS quality control
- Bisulfite conversion efficiency measurement
- Bioinformatics pipeline validation
- Targeted methyl-seq controls
