Falcon® 353025 | 150mm TC-Treated Cell Culture Dish with 20mm Molded Grid, Sterile — 10/Pack, 100/Case

The Falcon® 353025 is a 150mm TC-treated cell culture dish with a 20mm molded grid — the large-format, gridded cell culture dish for high-yield mammalian cell culture, large-scale protein expression, viral vector production, and any application requiring maximum growth surface area in a standard dish format. Falcon 150mm Cell Culture Dish with 20mm molded grid, TC-treated polystyrene, sterile. Actual Dimensions — 142.57mm O.D. × 24.77mm. Flat, optically clear polystyrene surfaces for distortion-free microscopic visualization of cells. Uniform surface chemistry created by vacuum-gas plasma treatment promotes cell attachment. Improved handling of small dishes with the unique Easy-Grip design. Capacity: 45mL. 10 dishes per pack, 100 dishes per case.

20mm Molded Grid — Precise Location Reference for Every Point on the Dish The defining feature of the 353025 is its 20mm molded Integrid™ grid — a permanent grid pattern molded directly into the base of the dish that divides the 150mm growth surface into a reference coordinate system. Unlike printed or ink-applied grids that can fade or wash off during media changes, the molded grid is a permanent structural feature of the polystyrene base — visible throughout the full life of the dish without degradation. The 20mm grid spacing is ideal for:

• Colony counting — counting and recording discrete colony positions in clonogenic assays, colony formation assays, and bacterial/yeast colony counting workflows
• Cell foci detection — mapping and tracking transformation foci, viral plaques, and other discrete colony or focus events across the dish surface
• Spatial tracking — recording the position of specific cells, colonies, or regions of interest for return visits to the same location across multiple imaging sessions
• Scratch/wound healing assays — creating reproducible scratch starting positions along grid lines and returning to the same field of view at each time point
• Limiting dilution cloning — confirming single-cell colony origin by recording colony position coordinates at early time points

Vacuum-Gas Plasma TC Treatment — Consistent, Uniform Cell Attachment Surface These dishes are vacuum-gas plasma treated for a consistent growth surface. The vacuum-gas plasma TC treatment creates a uniformly hydrophilic, negatively charged surface across the entire 150mm growth area — promoting consistent cell attachment, spreading, and proliferation across the full dish surface without the uneven attachment patterns that can result from inconsistent surface treatment. The uniformity of the plasma treatment is tightly controlled from dish to dish — ensuring consistent experimental results across the entire case.

Flat, Optically Clear Polystyrene — Distortion-Free Microscopic Visualization Features flat, optically clear polystyrene surfaces for distortion-free microscopic visualization of cells. The flat polystyrene base of the 353025 provides an optically clear, uniform focal plane across the entire 150mm growth surface — allowing direct inverted microscopic observation of cell morphology, confluency, grid-referenced colony positions, and dish contents without removing the dish from the incubator or disturbing the culture. The optical clarity is essential for colony counting and foci detection applications where precise visual resolution of individual colonies through the dish base is a workflow requirement.

Easy-Grip Design — Improved Handling of Large-Format Dishes Improved handling of small dishes with the unique Easy-Grip design. The Easy-Grip design provides an ergonomic grip feature on the dish body that improves the security of one-handed handling — particularly important for the 150mm format where the larger, heavier dish filled with 45mL of media requires more secure handling than smaller dish formats. The Easy-Grip reduces the risk of dropping dishes during transfer between incubator and biosafety cabinet.

Vented Lid — Consistent Gas Exchange During Incubation Special venting features associated with the lid facilitate consistent gas exchange during incubation. The vented lid of the 353025 allows controlled passive CO&sub2; and O&sub2; exchange between the dish interior and the incubator atmosphere — maintaining the correct gas balance for pH regulation and aerobic cell culture without requiring the lid to be cracked or partially removed. The vented design eliminates the contamination risk and inconsistent gas exchange associated with the cracked-lid method.

Stacking Rings — Stable, Space-Efficient Incubator Storage Stacking rings allow for easy handling and more stable stacking of dishes. The stacking rings molded into the lid and base of the 353025 create stable, self-aligning stacks of multiple dishes in the incubator — preventing sliding, tipping, and dish separation that could displace cell cultures from the growth surface and contaminate the incubator. For labs running multiple large-format dishes simultaneously, the stacking rings allow efficient vertical use of incubator shelf space.

Gamma Irradiation Sterile — Nonpyrogenic Sterilized by gamma irradiation, nonpyrogenic. Every 353025 is sterile by gamma irradiation and nonpyrogenic — qualifying it for sensitive mammalian cell culture, primary cell culture, viral vector production, and any application where endotoxin contamination from the dish material would affect cell viability, immune cell activation, or downstream assay results.

EcoChoice™ — Renewable Energy Manufacturing One or more facilities manufacturing this product is 100% covered by renewable energy through the purchase of unbundled energy attribute certificates (EACs).

Compatible Replacement — Cross-Reference Guide

The Falcon® 353025 is a direct compatible replacement for 150mm gridded TC-treated cell culture dishes from several major manufacturers:

• Thermo Fisher Nunc™ 168381 — Nunc 150mm dish with grid, TC-treated, sterile
• Greiner Bio-One 639161 — 150mm cell culture dish with grid, TC-treated, sterile
• Sarstedt 83.3903.300 — 150mm cell culture dish with grid, TC-treated, sterile

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific application and protocol.

Complete Falcon® Cell Culture Dish Family — Key Formats:

Key Features

• 150mm TC-treated cell culture dish with 20mm molded Integrid™ grid
• Molded grid — permanent, will not fade or wash off
• 20mm grid spacing — ideal for colony counting, foci detection, spatial tracking
• Vacuum-gas plasma TC treatment — uniform, consistent cell attachment surface
• Flat, optically clear polystyrene — distortion-free microscopic visualization
• Easy-Grip design — improved secure handling of large-format dish
• Vented lid — consistent CO&sub2;/O&sub2; exchange during incubation
• Stacking rings — stable multi-dish incubator stacking
• Actual dimensions: 142.57mm O.D. × 24.77mm
• Capacity: 45mL
• Growth area: approximately 148cm²
• Nonpyrogenic
• Sterile — gamma irradiation
• 10 dishes per pack / 100 dishes per case
• Corning EcoChoice™ — renewable energy manufacturing

Supported Applications Colony formation assays, clonogenic survival assays, cell transformation and foci assays, viral plaque assays, large-scale mammalian cell expansion, protein expression from adherent cell lines, viral vector production (adenoviral, lentiviral, adeno-associated virus), primary cell isolation and expansion, stem cell culture at scale, scratch/wound healing assays requiring grid-referenced field positions, limiting dilution cloning with spatial coordinate tracking, large-scale DNA and RNA harvest, and any mammalian cell culture application requiring a large-format 150mm TC-treated dish with a permanent reference grid.

Technical Specifications