Product Description
LIGHT LABS is proud to offer Mediatech DMEM — Dulbecco's Modification of Eagle's Medium (Cat # 10-013-CV) — one of the most widely used and scientifically validated liquid cell culture media formulations in the world, available in a convenient 6 x 500 mL case format for routine mammalian cell culture applications. Dulbecco's Modification of Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was developed to culture embryonic mouse cells and has since been extensively modified and validated to support a broad range of normal and transformed mammalian cell types, including primary cultures of mouse, chicken, human, hamster, and monkey cells.
The 10-013-CV formulation contains 4.5 g/L glucose — the high-glucose formulation widely preferred for rapidly dividing cell lines that consume glucose at high rates — along with L-glutamine as an energy source and nitrogen donor, and sodium pyruvate as an additional energy substrate that supports mitochondrial metabolism and cell growth. A key technical advantage of the Mediatech DMEM formulation is the substitution of Pyridoxine HCl for Pyridoxal — a critical formulation refinement that ensures significantly improved stability over the original DMEM formulation, reducing medium degradation during storage and extending the functional shelf life of each bottle.
Every production lot of Mediatech DMEM undergoes a comprehensive battery of quality control testing including growth promotion and cytotoxicity analysis, endotoxin testing by the LAL chromogenic assay (<0.25 EU/mL release specification), sterility testing, and mycoplasma testing — with a complete Certificate of Analysis and full formulation information available for each production lot. Manufactured in a facility that complies with an ISO 13485 certified quality management system. LIGHT LABS is your trusted source for Mediatech cell culture media and reagents.
KEY FEATURES
- High-glucose DMEM formulation — 4.5 g/L glucose; preferred for rapidly dividing cell lines with high glucose consumption rates
- L-glutamine included — essential energy source and nitrogen donor for mammalian cell growth and protein synthesis
- Sodium pyruvate included — additional energy substrate supporting mitochondrial metabolism and cell growth
- Pyridoxine HCl substituted for Pyridoxal — key formulation improvement ensuring significantly improved stability over the original DMEM formulation; reduced degradation during storage
- Comprehensive lot-specific quality control — growth promotion and cytotoxicity testing, endotoxin (<0.25 EU/mL by LAL chromogenic assay), sterility, and mycoplasma testing on every lot
- Complete Certificate of Analysis available for each production lot — full traceability and formulation documentation
- Manufactured under ISO 13485 certified quality management system
- Liquid, sterile, ready-to-use format — no reconstitution required; open and use
- Storage: 2°C to 8°C
- 500 mL bottles — convenient single-use or multi-use volume for standard cell culture workflows
- 6 bottles per case — standard case format for routine cell culture labs
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | 10-013-CV |
| Brand | Mediatech |
| Product Name | DMEM — Dulbecco's Modification of Eagle's Medium |
| Formulation | High glucose (4.5 g/L), with L-glutamine, with sodium pyruvate |
| Glucose Concentration | 4.5 g/L |
| L-Glutamine | Yes — included |
| Sodium Pyruvate | Yes — included |
| Sodium Bicarbonate | Yes — included |
| Pyridoxine Source | Pyridoxine HCl (improved stability vs. Pyridoxal) |
| Format | Liquid, sterile, ready-to-use |
| Endotoxin Level | <0.25 EU/mL (LAL chromogenic assay) |
| Sterility Testing | Yes — each lot tested |
| Mycoplasma Testing | Yes — each lot tested |
| Quality System | ISO 13485 certified |
| Certificate of Analysis | Yes — lot-specific CoA and full formulation available |
| Volume | 500 mL per bottle |
| Packaging | 6 bottles per case |
| Storage Temperature | 2°C to 8°C |
DMEM FORMULATION VARIANTS — CHOOSING THE RIGHT MEDIATECH DMEM
| Cat # | Glucose | L-Glutamine | Sodium Pyruvate | Best For |
|---|---|---|---|---|
| 10-013-CV | 4.5 g/L (High) | Yes | Yes | Most mammalian cell lines; rapidly dividing cells |
| 10-014-CV | 4.5 g/L (High) | No | Yes | Cells requiring glutamine-free or glutamine-added separately |
| 10-017-CV | 1.0 g/L (Low) | Yes | No | Low-glucose applications; adipocyte differentiation |
| 10-012-CV | 4.5 g/L (High) | Yes | No | Standard high-glucose without pyruvate |
APPLICATIONS
- Routine mammalian cell line maintenance and passaging
- Primary cell culture of mouse, chicken, human, hamster, and monkey cells
- Transformation and transfection experiments
- Viral production in adherent cell lines
- Protein expression in mammalian cell systems
- Cell-based assays requiring high-glucose DMEM
- Any standard mammalian cell culture application requiring liquid, ready-to-use high-glucose DMEM with L-glutamine and sodium pyruvate
