Product Description
LIGHT LABS is proud to offer Mediatech DMEM — Dulbecco's Modification of Eagle's Medium (Cat # 10-017-CV) — the sodium pyruvate-free formulation of the world's most widely used basal mammalian cell culture medium, providing 4.5 g/L high-glucose DMEM with L-glutamine but without sodium pyruvate, in a convenient 6 x 500 mL case format. The 10-017-CV is the preferred choice for researchers who need to control sodium pyruvate concentration independently — either because their experimental protocol calls for pyruvate-free basal media, because they add pyruvate at a defined concentration separately, or because their cell type or assay is sensitive to the presence of exogenous pyruvate in the basal medium.
The absence of sodium pyruvate in the 10-017-CV formulation is an important experimental variable. Sodium pyruvate serves as an alternative energy substrate that can be metabolized by mitochondria — its presence in the basal medium can influence cell metabolism, oxidative phosphorylation, and cellular responses to metabolic stress. In studies of mitochondrial function, oxidative stress, metabolic flux, or any experiment where precise control of available carbon sources is required, starting with a pyruvate-free basal medium (10-017-CV) and adding pyruvate only where specified gives researchers full control over this variable. The 10-017-CV is also the preferred starting formulation for custom media supplementation protocols where sodium pyruvate is pre-mixed into a supplement rather than the basal medium.
Like all Mediatech DMEM formulations, the 10-017-CV contains 4.5 g/L glucose and L-glutamine, and incorporates Pyridoxine HCl instead of Pyridoxal for improved stability. Every production lot undergoes comprehensive quality testing — growth promotion and cytotoxicity analysis, endotoxin testing by LAL chromogenic assay (<0.25 EU/mL), sterility testing, and mycoplasma testing — with a lot-specific Certificate of Analysis available. Manufactured under ISO 13485 certified quality management system. LIGHT LABS is your trusted source for Mediatech cell culture media and reagents.
KEY FEATURES
- High-glucose DMEM WITHOUT sodium pyruvate — 4.5 g/L glucose, L-glutamine; no sodium pyruvate; preferred when pyruvate is controlled independently or not required
- Critical experimental flexibility — sodium pyruvate-free basal medium allows precise independent control of pyruvate concentration; essential for metabolic studies, mitochondrial function research, and oxidative stress assays
- L-glutamine included — essential energy source and nitrogen donor for mammalian cell growth and protein synthesis
- Pyridoxine HCl substituted for Pyridoxal — key formulation improvement ensuring significantly improved stability over the original DMEM formulation
- Comprehensive lot-specific quality control — growth promotion and cytotoxicity testing, endotoxin (<0.25 EU/mL by LAL chromogenic assay), sterility, and mycoplasma testing on every lot
- Complete Certificate of Analysis available for each production lot — full traceability and formulation documentation
- Manufactured under ISO 13485 certified quality management system
- Liquid, sterile, ready-to-use format — no reconstitution required
- pH: 7.0–7.4
- 18-month shelf life
- Storage: 2°C to 8°C
- 500 mL bottles — convenient volume for standard cell culture workflows
- 6 bottles per case
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | 10-017-CV |
| Brand | Mediatech |
| Product Name | DMEM — Dulbecco's Modification of Eagle's Medium |
| Formulation | High glucose (4.5 g/L), with L-glutamine, WITHOUT sodium pyruvate |
| Glucose Concentration | 4.5 g/L |
| L-Glutamine | Yes — included |
| Sodium Pyruvate | No — NOT included |
| Sodium Bicarbonate | Yes — included |
| Pyridoxine Source | Pyridoxine HCl (improved stability vs. Pyridoxal) |
| Format | Liquid, sterile, ready-to-use |
| pH | 7.0–7.4 |
| Endotoxin Level | <0.25 EU/mL (LAL chromogenic assay) |
| Sterility Testing | Yes — each lot tested |
| Mycoplasma Testing | Yes — each lot tested |
| Quality System | ISO 13485 certified |
| Certificate of Analysis | Yes — lot-specific CoA and full formulation available |
| Shelf Life | 18 months |
| Volume | 500 mL per bottle |
| Packaging | 6 bottles per case |
| Storage Temperature | 2°C to 8°C |
10-013-CV vs. 10-017-CV — CHOOSING THE RIGHT MEDIATECH DMEM
| Feature | 10-013-CV | 10-017-CV |
|---|---|---|
| Glucose | 4.5 g/L | 4.5 g/L |
| L-Glutamine | Yes | Yes |
| Sodium Pyruvate | Yes — included | No — NOT included |
| Best For | Most routine cell culture applications | Pyruvate-sensitive assays; independent pyruvate control |
DMEM FORMULATION VARIANTS — COMPLETE MEDIATECH DMEM LINEUP
| Cat # | Glucose | L-Glutamine | Sodium Pyruvate | Best For |
|---|---|---|---|---|
| 10-013-CV | 4.5 g/L (High) | Yes | Yes | Most mammalian cell lines; rapidly dividing cells |
| 10-017-CV | 4.5 g/L (High) | Yes | No | Pyruvate-sensitive assays; independent pyruvate control |
| 10-014-CV | 4.5 g/L (High) | No | Yes | Glutamine-free applications; glutamine added separately |
| 10-012-CV | 4.5 g/L (High) | Yes | No | Standard high-glucose without pyruvate |
| 10-027-CV | 4.5 g/L (High) + 25 mM HEPES | Yes | No | Buffered DMEM for non-CO₂ environments |
APPLICATIONS
- Routine mammalian cell culture where sodium pyruvate is not required or is added separately
- Metabolic studies and mitochondrial function research requiring precise control of pyruvate availability
- Oxidative stress assays sensitive to exogenous sodium pyruvate in the basal medium
- Custom media supplementation protocols where pyruvate is pre-mixed in a supplement
- Cell culture applications requiring high-glucose DMEM with L-glutamine but without pyruvate
- Any standard mammalian cell culture application where the researcher requires pyruvate-free basal medium
