Product Description
LIGHT LABS is proud to offer Mediatech DMEM — Dulbecco's Modification of Eagle's Medium (Cat # 15-017-CV) — the most flexible basal DMEM formulation in the Mediatech lineup, providing 4.5 g/L high-glucose DMEM without L-glutamine and without sodium pyruvate in a convenient 6 x 500 mL case format. The 15-017-CV is the preferred choice for researchers who require maximum independent control over both glutamine and pyruvate supplementation — either because their experimental protocol requires adding these components at precisely defined concentrations, because they use stable glutamine substitutes such as GlutaMAX™ or Corning® GlutaGRO™ in place of standard L-glutamine, because their cell type is sensitive to glutamine-derived ammonia accumulation, or because both components must be strictly excluded from the basal medium for specific assay conditions.
The absence of both L-glutamine and sodium pyruvate in the 15-017-CV makes it the basal formulation of choice for several important experimental scenarios. Glutamine-free DMEM is essential for experiments studying glutamine metabolism, glutamine-dependent signaling pathways, or cell responses to glutamine deprivation — where the presence of glutamine in the basal medium would confound results. It is also the preferred starting point for researchers using GlutaMAX™ or Corning® GlutaGRO™ dipeptide glutamine substitutes, which provide the metabolic benefits of glutamine without the instability and ammonia generation of free L-glutamine. Combined with the absence of sodium pyruvate, the 15-017-CV gives researchers complete, independent control over all carbon and nitrogen source supplementation in their cell culture system.
Like all Mediatech DMEM formulations, the 15-017-CV incorporates Pyridoxine HCl instead of Pyridoxal for improved stability, and every production lot undergoes comprehensive quality testing with a lot-specific Certificate of Analysis. Manufactured under ISO 13485 certified quality management system. LIGHT LABS is your trusted source for Mediatech cell culture media and reagents.
KEY FEATURES
- High-glucose DMEM WITHOUT L-glutamine AND without sodium pyruvate — maximum experimental flexibility; complete independent control over both nitrogen source and carbon source supplementation
- Glutamine-free formulation — essential for glutamine metabolism studies, glutamine deprivation experiments, and glutamine-sensitive signaling assays
- Ideal starting medium for GlutaMAX™ or Corning® GlutaGRO™ users — add stable dipeptide glutamine substitute at defined concentration without glutamine present in the basal medium
- No ammonia generation from unstable free L-glutamine — particularly important for long-duration cultures and sensitive cell types
- Sodium pyruvate-free — provides complete independent control of pyruvate availability alongside glutamine-free formulation
- 4.5 g/L high-glucose formulation — standard high-glucose concentration for rapidly dividing mammalian cell lines
- Pyridoxine HCl substituted for Pyridoxal — improved stability over the original DMEM formulation
- Comprehensive lot-specific quality control — growth promotion and cytotoxicity testing, endotoxin (<0.25 EU/mL by LAL chromogenic assay), sterility, and mycoplasma testing on every lot
- Complete Certificate of Analysis available for each production lot
- Manufactured under ISO 13485 certified quality management system
- Liquid, sterile, ready-to-use format
- pH: 7.0–7.4
- 18-month shelf life
- Storage: 2°C to 8°C
- 500 mL bottles; 6 bottles per case
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | 15-017-CV |
| Brand | Mediatech |
| Product Name | DMEM — Dulbecco's Modification of Eagle's Medium |
| Formulation | High glucose (4.5 g/L); WITHOUT L-glutamine; WITHOUT sodium pyruvate |
| Glucose Concentration | 4.5 g/L |
| L-Glutamine | No — NOT included |
| Sodium Pyruvate | No — NOT included |
| Sodium Bicarbonate | Yes — included |
| Pyridoxine Source | Pyridoxine HCl (improved stability vs. Pyridoxal) |
| Format | Liquid, sterile, ready-to-use |
| pH | 7.0–7.4 |
| Endotoxin Level | <0.25 EU/mL (LAL chromogenic assay) |
| Sterility Testing | Yes — each lot tested |
| Mycoplasma Testing | Yes — each lot tested |
| Quality System | ISO 13485 certified |
| Certificate of Analysis | Yes — lot-specific CoA and full formulation available |
| Shelf Life | 18 months |
| Volume | 500 mL per bottle |
| Packaging | 6 bottles per case |
| Storage Temperature | 2°C to 8°C |
CHOOSING THE RIGHT MEDIATECH DMEM — GLUTAMINE & PYRUVATE OPTIONS
| Cat # | L-Glutamine | Sodium Pyruvate | Best For |
|---|---|---|---|
| 10-013-CV | Yes | Yes | Most routine mammalian cell culture |
| 10-017-CV | Yes | No | Pyruvate control; add pyruvate separately |
| 15-017-CV | No | No | Maximum flexibility; GlutaMAX™/GlutaGRO™; glutamine studies |
| 10-014-CV | No | Yes | Glutamine-free with pyruvate included |
APPLICATIONS
- Cell culture requiring maximum independent control of both L-glutamine and sodium pyruvate supplementation
- Glutamine metabolism studies and glutamine deprivation experiments
- Glutamine-dependent signaling pathway research
- Culture with GlutaMAX™ or Corning® GlutaGRO™ dipeptide glutamine substitutes
- Long-duration cultures sensitive to ammonia accumulation from L-glutamine degradation
- Custom media formulation and supplementation development
- Serum-free or chemically defined media formulation requiring precise nitrogen and carbon source control
- Any application where both L-glutamine and sodium pyruvate must be excluded from the basal DMEM formulation
ORDERING INFORMATION — MEDIATECH DMEM FORMULATIONS
| Cat # | Description | L-Gln | Pyruvate | Volume | Pkg |
|---|---|---|---|---|---|
| 15-017-CV | DMEM, High Glucose (4.5 g/L), No L-Glutamine, No Sodium Pyruvate | No | No | 500 mL | 6/case |
| 15-017-CM | DMEM, High Glucose (4.5 g/L), No L-Glutamine, No Sodium Pyruvate | No | No | 1 L | 6/case |
| 10-013-CV | DMEM, High Glucose (4.5 g/L), L-Glutamine, Sodium Pyruvate | Yes | Yes | 500 mL | 6/case |
| 10-017-CV | DMEM, High Glucose (4.5 g/L), L-Glutamine, No Sodium Pyruvate | Yes | No | 500 mL | 6/case |
| 10-014-CV | DMEM, High Glucose (4.5 g/L), No L-Glutamine, Sodium Pyruvate | No | Yes | 500 mL | 6/case |
| 10-092-CV | DMEM/Ham's F-12 50/50 Mix, L-Glutamine, 15 mM HEPES | Yes | No | 500 mL | 6/case |
